向日葵Trihelix转录因子家族鉴定及表达分析

张曼, 董海潇, 田娟, 冯博, 于学鹏, 王祉诺, 张雷, 巴桑平措, 李雪英, 李慧英

Identification and Expression Analysis of Trihelix Transcription Factor Family in Sunflower

Zhang Man, Dong Haixiao, Tian Juan, Feng Bo, Yu Xuepeng, Wang Zhinuo, Zhang Lei, Basangpingcuo , Li Xueying, Li Huiying

表1 qRT-PCR所需引物

Table 1 Primers required for qRT-PCR

基因名称Gene name 正向引物（5’-3’）Forward primer (5’-3’) 反向引物（5’-3’）Reverse primer (5’-3’) HaTrihelix6 GCCGTGGCAGAGGATGAA GCCGTATCGGAAACTTGAT HaTrihelix12 ACGTCGTCTCAGCTCCATC TCACCGTCATCAGTAAATCCA HaTrihelix15 TTGAAGCGATTGTGAAAGAG TGCCGAGACAGACGATAA HaTrihelix16 ATGACTGAACCTCCACCG CAAATCCCTCGTCTCCTG HaTrihelix19 ACAATGCGGGAAGTAGAG GCGAAGAGCTTAGCGATC HaTrihelix20 CCACCACAACCGACACCA CGGATACCGATACAGATACAGGA HaTrihelix26 ACCCGAACCCTGATGTGA CCCTCGCCAACTCCTTAT HaTrihelix27 TGAATGAAACCGCTCTGC ACCGTTGTCATCATCCCT HaTrihelix28 GCCCGCAATGCAGTTATT GTCGGTTGTGCTGGTGGT HaTrihelix31 CCACCAAGTCAGCCTCATG CAGCACTCAGACTCGCAGTT 18S rRNA CTACCACATCCAAGGAAGGCAG CGACAGAAGGGACGAGTAAACC