利用CRISPR/Cas9技术编辑Badh2基因改良水稻优质恢复系QR79的香味

吴钱蓉, 吴娴, 姜雪, 王倩, 宫彦龙, 王忠妮, 朱速松, 宋莉

CRISPR/Cas9-Mediated Editing of the Badh2 Gene Improves Aroma in Elite Restorer Line QR79

Wu Qianrong, Wu Xian, Jiang Xue, Wang Qian, Gong Yanlong, Wang Zhongni, Zhu Susong, Song Li

图2 靶位点在Badh2基因上的位置及载体构建图 (a) 优质恢复系QR79香味基因Badh2全长序列对比图，NIP：日本晴，CON：共识序列，下同；(b) 2个靶位点在Badh2基因上的位置，黑色区域为外显子区域，左一为第1外显子，左二为第2外显子，红色序列为靶点序列，蓝色序列为PAM序列；(c) U6a驱动的T1和U6b驱动的T2 2个表达盒在pEGCas9Pubi-H-Badh2载体上的连接顺序。

Fig.2 The position of the target site on the Badh2 gene and the vector construction diagram (a) full-length sequence alignment of fragrance gene Badh2 in elite restorer line QR79, NIP: Nipponbare, CON: consensus, the same below; (b) positions of two target sites on the Badh2 gene. Black regions represent exons (the leftmost one is Exon 1, the second from the left is Exon 2); red sequences indicate target sequences, and blue sequences denote PAM sequences; (c) assembly order of two expression cassettes (T1 driven by U6a and T2 driven by U6b) on vector pEGCas9Pubi-H-Badh2.