利用CRISPR/Cas9技术编辑Badh2基因改良水稻优质恢复系QR79的香味

吴钱蓉, 吴娴, 姜雪, 王倩, 宫彦龙, 王忠妮, 朱速松, 宋莉

CRISPR/Cas9-Mediated Editing of the Badh2 Gene Improves Aroma in Elite Restorer Line QR79

Wu Qianrong, Wu Xian, Jiang Xue, Wang Qian, Gong Yanlong, Wang Zhongni, Zhu Susong, Song Li

图3 T0代转基因阳性植株鉴定及其突变类型分析 (a) Cas9-F/R引物对T0代阳性转基因植株PCR检测；编号1~12对应材料J-18、J-19、J-22、J-23、J-25、J-26、J-27、J-29、J-32、J-阴-1、J-阴-2、J-阴-3；“+”代表阳性对照，“-”代表阴性对照，M代表2000 bp DNA marker，下同。(b)和(c)分别为基因编辑株系T0代单株靶点1和靶点2突变类型分析；红色字体表示靶点序列，蓝色字体表示PAM序列，“-”表示碱基缺失，黑色加粗表示碱基突变。

Fig.3 Identification of T0 generation transgenic positive plants and analysis of their mutation types (a) PCR detection of T? positive transgenic plants using Cas9-F/R primer pairs; Numbers 1-12 correspond to materials J-18, J-19, J-22, J-23, J-25, J-26, J-27, J-29, J-32, J-Neg-1, J-Neg-2, J-Neg-3;“+”denotes positive control,“-”denotes negative control, and M represents 2000 bp DNA marker, the same below. (b) and (c) Mutation pattern analysis at Target 1 and Target 2 of individual T? gene-edited lines, respectively; red fonts indicate target sequences, blue fonts indicate PAM sequences,“-”represents base deletions, and bold black fonts represent base substitutions.