利用CRISPR/Cas9技术编辑Badh2基因改良水稻优质恢复系QR79的香味

吴钱蓉, 吴娴, 姜雪, 王倩, 宫彦龙, 王忠妮, 朱速松, 宋莉

CRISPR/Cas9-Mediated Editing of the Badh2 Gene Improves Aroma in Elite Restorer Line QR79

Wu Qianrong, Wu Xian, Jiang Xue, Wang Qian, Gong Yanlong, Wang Zhongni, Zhu Susong, Song Li

图4 Cas9-F/R引物对T1代基因编辑株系中Cas9基因PCR检测图 (a) 编号1~8对应J-18株系；(b) 编号1~8对应J-29株系；(c) T1代纯合突变植株序列对比图。

Fig.4 The PCR detection diagram of the Cas9 gene in the T1 generation gene-edited lines using the Cas9-F/R primer pair (a) Number 1-8 correspond to line J-18; (b) Number 1-8 correspond to line J-29; (c) Sequence alignment of homozygous mutant plants in the T? generation.