苎麻ABC转运蛋白基因家族鉴定及其镉胁迫表达模式分析
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顾一舟, 颜世伟, 朱爱国, 陈坤梅, 陈佳, 陈继康, 高钢, 王晓飞, 吴独清, 杨桢, 陈平
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Identification of the ABC Transporter Protein Gene Family and Expression Pattern Analysis under Cadmium Stress in Ramie (Boehmeria nivea)
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Gu Yizhou, Yan Shiwei, Zhu Aiguo, Chen Kunmei, Chen Jia, Chen Jikang, Gao Gang, Wang Xiaofei, Wu Duqing, Yang Zhen, Chen Ping
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表2 苎麻ABC转运蛋白家族成员理化性质及亚细胞定位预测
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Table 2 Physicochemical properties and subcellular localization prediction of ABC transporter family members in B. nivea
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基因名称 Gene name | 氨基酸数目 Number of amino acids | 分子量 Molecular weight (Da) | 理论等电点 Theoretical pI | 不稳定指数 Instability index | 脂肪族指数 Aliphatic index | 总平均亲水范围 Grand average of hydropathicity | 亚细胞定位 Subcellular localization | | BnABC1 | 329 | 35 786.23 | 5.85 | 57.57 | 102.55 | -0.005 | 叶绿体 | | BnABC10 | 1067 | 119 303.23 | 9.05 | 43.89 | 87.47 | -0.183 | 细胞膜 | | BnABC100 | 829 | 93 102.28 | 9.23 | 38.57 | 88.77 | -0.115 | 细胞膜 | | BnABC101 | 1471 | 165 685.83 | 7.53 | 39.45 | 95.03 | 0.068 | 细胞膜 | | BnABC102 | 1443 | 163 270.31 | 7.88 | 40.00 | 96.80 | 0.052 | 细胞膜 | | BnABC103 | 2121 | 237 315.39 | 8.52 | 36.70 | 89.50 | -0.025 | 细胞膜 | | BnABC104 | 954 | 105 228.38 | 8.05 | 37.90 | 92.65 | 0.077 | 细胞膜 | | BnABC105 | 698 | 78 573.22 | 9.29 | 36.90 | 92.11 | -0.027 | 叶绿体 | | BnABC106 | 966 | 108 062.89 | 8.49 | 36.90 | 95.51 | 0.088 | 细胞膜 | | BnABC107 | 630 | 68 252.87 | 7.71 | 31.98 | 109.97 | 0.142 | 细胞膜 | | BnABC108 | 1279 | 138 484.91 | 8.31 | 33.28 | 101.16 | 0.153 | 细胞膜 | | BnABC109 | 229 | 25 909.59 | 9.62 | 42.20 | 108.17 | -0.089 | 叶绿体 | | BnABC11 | 761 | 83 637.71 | 9.57 | 47.15 | 97.56 | 0.148 | 细胞膜 | | BnABC110 | 266 | 29 190.96 | 6.61 | 28.42 | 114.29 | -0.006 | 细胞质 | | BnABC111 | 1262 | 137 845.95 | 9.08 | 39.08 | 96.33 | 0.056 | 细胞膜 | | BnABC112 | 723 | 79 490.08 | 9.48 | 36.79 | 107.21 | 0.296 | 细胞膜 | | BnABC113 | 717 | 81 714.16 | 8.87 | 45.76 | 92.83 | 0.011 | 细胞膜 | | BnABC114 | 166 | 17 960.95 | 9.94 | 21.57 | 89.88 | -0.040 | 叶绿体 | | BnABC115 | 299 | 33 831.50 | 9.58 | 33.39 | 96.82 | 0.419 | 细胞膜 | | BnABC116 | 718 | 79 999.54 | 5.72 | 36.10 | 82.47 | -0.607 | 细胞质 | | BnABC117 | 677 | 75 129.64 | 8.52 | 42.43 | 89.07 | 0.020 | 细胞膜 | | BnABC118 | 748 | 82 933.77 | 8.81 | 41.33 | 91.32 | 0.054 | 细胞膜 | | BnABC119 | 118 | 12 434.19 | 9.24 | 35.33 | 72.54 | -0.464 | 细胞膜 | | BnABC12 | 707 | 76 741.94 | 6.11 | 34.37 | 100.01 | 0.176 | 细胞膜 | | BnABC120 | 507 | 57 192.68 | 8.86 | 42.30 | 92.64 | 0.180 | 细胞膜 | | BnABC121 | 150 | 15 627.12 | 4.77 | 46.07 | 64.87 | -0.413 | 细胞质 | | BnABC122 | 724 | 80 566.04 | 8.64 | 42.66 | 90.43 | 0.044 | 细胞膜 | | BnABC123 | 605 | 68 253.71 | 8.03 | 32.14 | 95.65 | -0.224 | 细胞质 | | BnABC124 | 456 | 50 255.76 | 9.76 | 51.31 | 85.13 | 0.071 | 细胞膜 | | BnABC125 | 173 | 18 659.63 | 9.65 | 24.18 | 90.75 | 0.025 | 叶绿体 | | BnABC126 | 1427 | 160 469.18 | 8.02 | 40.79 | 100.37 | 0.124 | 细胞膜 | | BnABC127 | 1399 | 157 363.60 | 6.89 | 40.44 | 99.35 | 0.136 | 细胞膜 | | BnABC128 | 1437 | 162 922.61 | 7.80 | 40.48 | 91.60 | 0.036 | 细胞膜 | | BnABC129 | 700 | 76 959.98 | 9.41 | 40.54 | 92.23 | 0.041 | 细胞膜 | | BnABC13 | 764 | 83 866.95 | 9.57 | 47.00 | 97.43 | 0.151 | 细胞膜 | | BnABC130 | 700 | 78 410.17 | 9.16 | 45.41 | 92.87 | 0.057 | 细胞膜 | | BnABC131 | 1838 | 205 293.03 | 7.08 | 36.32 | 93.85 | 0.040 | 细胞膜 | | BnABC132 | 96 | 10 344.68 | 9.21 | 62.96 | 76.15 | -0.417 | 细胞质 | | BnABC133 | 633 | 71 347.54 | 9.08 | 44.09 | 99.30 | 0.221 | 细胞膜 | | BnABC134 | 1931 | 215 756.59 | 6.80 | 34.86 | 95.94 | 0.090 | 细胞膜 | | BnABC135 | 2192 | 243 148.60 | 5.98 | 42.72 | 83.95 | -0.238 | 细胞质 | | BnABC136 | 639 | 70 662.25 | 9.25 | 37.76 | 110.23 | 0.164 | 细胞膜 | | BnABC137 | 1359 | 153 858.44 | 6.01 | 36.70 | 96.40 | 0.120 | 细胞膜 | | BnABC138 | 1361 | 153 484.27 | 8.47 | 38.00 | 93.09 | 0.084 | 细胞膜 | | BnABC139 | 790 | 89 381.72 | 6.74 | 35.00 | 96.03 | 0.196 | 细胞膜 | | BnABC14 | 1107 | 123 621.15 | 9.08 | 43.53 | 88.63 | -0.182 | 细胞膜 | | BnABC140 | 1325 | 144 689.71 | 8.17 | 35.93 | 94.44 | 0.034 | 细胞膜 | | BnABC141 | 1063 | 118 402.84 | 9.71 | 56.10 | 80.15 | -0.283 | 细胞膜 | | BnABC142 | 693 | 75 861.19 | 8.29 | 41.79 | 97.37 | 0.047 | 细胞膜 | | BnABC143 | 956 | 107 912.18 | 8.17 | 43.32 | 104.93 | 0.076 | 细胞膜 | | BnABC15 | 1985 | 216 409.71 | 9.22 | 46.55 | 85.36 | -0.291 | 细胞膜 | | BnABC16 | 654 | 73 534.47 | 8.47 | 39.94 | 105.63 | 0.140 | 细胞膜 | | BnABC17 | 1499 | 169 483.22 | 8.69 | 39.44 | 88.01 | 0.000 | 细胞膜 | | BnABC18 | 1456 | 161 252.28 | 7.68 | 40.35 | 109.08 | 0.235 | 细胞膜 | | BnABC19 | 1374 | 149 492.74 | 9.02 | 42.61 | 92.08 | -0.031 | 细胞膜 | | BnABC2 | 329 | 35 802.29 | 5.85 | 56.98 | 102.55 | 0.005 | 叶绿体 | | BnABC20 | 1410 | 157 000.54 | 6.22 | 43.16 | 105.17 | 0.221 | 细胞膜 | | BnABC21 | 1427 | 161 808.11 | 8.82 | 42.61 | 91.87 | 0.006 | 细胞膜 | | BnABC22 | 1414 | 159 175.66 | 6.68 | 35.91 | 92.18 | 0.043 | 细胞膜 | | BnABC23 | 661 | 71 375.06 | 8.86 | 38.29 | 99.03 | 0.096 | 细胞膜 | | BnABC24 | 1647 | 184 320.56 | 6.16 | 36.09 | 102.40 | 0.070 | 液泡 | | BnABC25 | 1623 | 182 766.32 | 7.78 | 41.35 | 104.74 | 0.043 | 液泡 | | BnABC26 | 1504 | 168 461.41 | 8.94 | 39.25 | 106.43 | 0.113 | 液泡 | | BnABC27 | 585 | 65 618.21 | 6.50 | 31.46 | 77.68 | -0.394 | 叶绿体 | | BnABC28 | 1249 | 134 406.97 | 8.84 | 40.52 | 108.29 | 0.245 | 细胞膜 | | BnABC29 | 346 | 37 862.32 | 6.47 | 42.50 | 90.38 | -0.158 | 叶绿体 | | BnABC3 | 691 | 77 745.35 | 5.87 | 42.27 | 97.50 | -0.122 | 细胞膜 | | BnABC30 | 598 | 67 041.82 | 6.11 | 35.72 | 90.90 | -0.332 | 叶绿体 | | BnABC31 | 687 | 76 115.42 | 9.03 | 45.07 | 100.17 | 0.207 | 细胞膜 | | BnABC32 | 1113 | 122 855.57 | 9.03 | 37.63 | 87.01 | -0.105 | 细胞膜 | | BnABC33 | 597 | 66 855.55 | 6.03 | 35.26 | 91.39 | -0.326 | 细胞质 | | BnABC34 | 695 | 76 990.44 | 8.94 | 44.15 | 100.00 | 0.204 | 细胞膜 | | BnABC35 | 1171 | 129 630.69 | 9.06 | 36.60 | 88.11 | -0.091 | 细胞膜 | | BnABC36 | 1325 | 149 128.21 | 9.31 | 47.67 | 98.91 | -0.075 | 细胞膜 | | BnABC37 | 702 | 78 293.93 | 9.11 | 40.28 | 88.63 | 0.055 | 细胞膜 | | BnABC38 | 678 | 75 331.68 | 9.15 | 44.01 | 97.05 | 0.168 | 细胞膜 | | BnABC39 | 1521 | 171 581.56 | 8.99 | 39.39 | 93.57 | 0.024 | 细胞膜 | | BnABC4 | 704 | 78 327.37 | 8.98 | 39.53 | 95.28 | 0.110 | 细胞膜 | | BnABC40 | 750 | 82 406.44 | 9.47 | 33.27 | 95.08 | -0.015 | 细胞膜 | | BnABC41 | 1365 | 153 898.34 | 7.35 | 42.98 | 96.29 | -0.038 | 细胞膜 | | BnABC42 | 1454 | 164 236.73 | 7.17 | 43.02 | 98.25 | 0.016 | 细胞膜 | | BnABC43 | 331 | 36 795.42 | 8.29 | 46.73 | 93.99 | -0.220 | 细胞质 | | BnABC44 | 680 | 75 770.76 | 7.96 | 35.20 | 95.32 | 0.167 | 细胞膜 | | BnABC45 | 707 | 78 368.68 | 8.53 | 33.30 | 94.47 | 0.130 | 细胞膜 | | BnABC46 | 663 | 74 173.97 | 8.97 | 39.05 | 98.07 | 0.108 | 细胞膜 | | BnABC47 | 715 | 77 839.38 | 6.23 | 34.38 | 100.66 | 0.189 | 细胞膜 | | BnABC48 | 1411 | 155 935.63 | 6.14 | 48.48 | 101.51 | 0.065 | 细胞膜 | | BnABC49 | 663 | 74 157.97 | 8.97 | 39.70 | 98.07 | 0.106 | 细胞膜 | | BnABC5 | 715 | 77 465.97 | 9.42 | 35.64 | 102.81 | 0.154 | 细胞膜 | | BnABC50 | 1409 | 158 669.39 | 8.86 | 37.04 | 94.46 | 0.095 | 细胞膜 | | BnABC51 | 1440 | 162 701.85 | 7.06 | 33.05 | 90.81 | 0.034 | 细胞膜 | | BnABC52 | 1399 | 157 653.09 | 8.78 | 35.94 | 94.44 | 0.099 | 细胞膜 | | BnABC53 | 1440 | 162 826.11 | 7.06 | 32.81 | 90.40 | 0.033 | 细胞膜 | | BnABC54 | 1443 | 163 131.34 | 7.27 | 33.61 | 90.46 | 0.018 | 细胞膜 | | BnABC55 | 1526 | 169 520.35 | 7.30 | 36.66 | 103.86 | 0.122 | 细胞膜 | | BnABC56 | 1451 | 160 926.16 | 7.26 | 34.36 | 102.72 | 0.100 | 细胞膜 | | BnABC57 | 611 | 68 341.51 | 9.09 | 50.23 | 104.84 | 0.195 | 细胞膜 | | BnABC58 | 604 | 64 942.14 | 8.18 | 31.59 | 109.35 | 0.241 | 细胞膜 | | BnABC59 | 1253 | 136 569.98 | 8.43 | 36.26 | 99.73 | 0.122 | 细胞膜 | | BnABC6 | 618 | 68 677.07 | 9.00 | 47.24 | 116.13 | 0.378 | 细胞膜 | | BnABC60 | 1416 | 161 136.58 | 8.77 | 39.93 | 93.06 | -0.027 | 细胞膜 | | BnABC61 | 1214 | 138 282.66 | 8.14 | 39.33 | 96.43 | 0.037 | 细胞膜 | | BnABC62 | 275 | 29 914.70 | 8.97 | 36.90 | 96.80 | 0.304 | 细胞膜 | | BnABC63 | 401 | 44 158.42 | 9.41 | 32.76 | 106.48 | 0.143 | 细胞膜 | | BnABC64 | 138 | 15 331.65 | 8.84 | 32.18 | 89.06 | -0.234 | 细胞膜 | | BnABC65 | 1240 | 133 857.61 | 7.05 | 41.09 | 97.89 | 0.089 | 细胞膜 | | BnABC66 | 1294 | 141 492.68 | 8.52 | 45.09 | 94.91 | 0.006 | 细胞膜 | | BnABC67 | 472 | 51 962.45 | 6.44 | 41.60 | 95.21 | -0.062 | 细胞膜 | | BnABC68 | 658 | 73 509.27 | 9.44 | 43.50 | 109.53 | 0.086 | 细胞膜 | | BnABC69 | 160 | 18 189.44 | 5.41 | 43.29 | 120.62 | 0.080 | 细胞膜 | | BnABC7 | 399 | 44 315.80 | 5.56 | 43.81 | 96.04 | -0.049 | 细胞膜 | | BnABC70 | 265 | 29 216.26 | 9.04 | 52.27 | 98.72 | 0.325 | 细胞膜 | | BnABC71 | 1318 | 143 829.35 | 8.67 | 43.37 | 93.56 | -0.005 | 细胞膜 | | BnABC72 | 1498 | 167 470.87 | 6.63 | 32.82 | 108.15 | 0.256 | 细胞膜 | | BnABC73 | 1140 | 128 564.11 | 7.57 | 39.56 | 105.86 | 0.117 | 细胞膜 | | BnABC74 | 1485 | 166 786.94 | 7.69 | 40.34 | 106.67 | 0.201 | 细胞膜 | | BnABC75 | 1228 | 134 490.45 | 8.61 | 35.05 | 103.88 | 0.114 | 细胞膜 | | BnABC76 | 478 | 51 808.60 | 6.03 | 50.00 | 85.46 | -0.260 | 叶绿体 | | BnABC77 | 709 | 79 001.04 | 5.70 | 37.13 | 89.79 | -0.263 | 叶绿体 | | BnABC78 | 1518 | 168 062.72 | 8.07 | 37.84 | 105.61 | 0.204 | 细胞膜 | | BnABC79 | 108 | 12 443.61 | 5.00 | 41.89 | 112.04 | -0.203 | 细胞质 | | BnABC8 | 1473 | 164 206.31 | 8.23 | 36.14 | 103.61 | 0.136 | 细胞膜 | | BnABC80 | 284 | 31 495.17 | 6.08 | 47.56 | 91.62 | -0.132 | 细胞质 | | BnABC81 | 1505 | 168 501.65 | 8.39 | 37.23 | 103.36 | 0.163 | 细胞膜 | | BnABC82 | 675 | 74 654.83 | 7.98 | 37.69 | 92.92 | 0.036 | 细胞膜 | | BnABC83 | 150 | 16 509.76 | 4.87 | 42.84 | 94.93 | -0.099 | 细胞质 | | BnABC84 | 731 | 79 316.15 | 8.76 | 39.43 | 92.38 | -0.029 | 细胞膜 | | BnABC85 | 264 | 29 407.77 | 5.34 | 37.81 | 93.11 | -0.035 | 细胞膜 | | BnABC86 | 605 | 68 280.74 | 7.80 | 36.37 | 95.34 | -0.230 | 细胞质 | | BnABC87 | 304 | 34 736.06 | 9.20 | 37.45 | 107.11 | 0.373 | 高尔基体 | | BnABC88 | 716 | 78 153.08 | 9.07 | 36.99 | 93.80 | 0.087 | 细胞膜 | | BnABC89 | 461 | 51 398.71 | 8.97 | 39.00 | 105.75 | 0.258 | 细胞膜 | | BnABC9 | 1263 | 139 196.94 | 8.78 | 43.68 | 101.41 | 0.045 | 细胞膜 | | BnABC90 | 1252 | 137 490.25 | 9.36 | 37.03 | 97.84 | 0.042 | 细胞膜 | | BnABC91 | 1426 | 159 285.37 | 8.40 | 35.73 | 104.87 | 0.102 | 细胞膜 | | BnABC92 | 1208 | 131 757.85 | 9.15 | 38.42 | 96.67 | 0.061 | 细胞膜 | | BnABC93 | 1422 | 161 910.16 | 8.18 | 39.01 | 93.65 | 0.067 | 细胞膜 | | BnABC94 | 602 | 67 293.34 | 8.97 | 42.36 | 103.90 | 0.228 | 细胞膜 | | BnABC95 | 1422 | 161 910.20 | 8.30 | 38.77 | 93.52 | 0.065 | 细胞膜 | | BnABC96 | 1281 | 137 921.08 | 8.71 | 34.63 | 99.32 | 0.135 | 细胞膜 | | BnABC97 | 1281 | 138 054.31 | 8.65 | 35.15 | 100.01 | 0.146 | 细胞膜 | | BnABC98 | 723 | 80 728.35 | 6.40 | 39.43 | 86.13 | -0.451 | 细胞质 | | BnABC99 | 1248 | 135 387.58 | 8.60 | 33.95 | 107.35 | 0.208 | 细胞膜 |
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