Crops ›› 2024, Vol. 40 ›› Issue (1): 233-238.doi: 10.16035/j.issn.1001-7283.2024.01.031

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Molecular Detection and Identification of Maize Yellow Mosaic Virus on Sorghum in Huai’an, Jiangsu

Wang Haitao(), Ren Chunmei, Dong Yan, Li Shuo, Cheng Zhaobang, Ji Yinghua()   

  1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences / Key Laboratory of Food Quality and Safety of Jiangsu Province/State Key Laboratory Breeding Base, Nanjing 210014, Jiangsu, China
  • Received:2022-12-16 Revised:2023-09-22 Online:2024-02-15 Published:2024-02-20
  • Contact: Ji Yinghua E-mail:wanghaitao9105@163.com;jiyinghua@jaas.ac.cn

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Abstract:

In order to understand the incidence of sorghum virus disease in Jiangsu province, the 20 suspected sorghum virus disease samples were collected from Huai’an city, and molecular detection and identification were carried out. The results showed that the bands consistent with the target fragment could be amplified by using the maize yellow mosaic virus (MaYMV) primer, and the detection rate was 80%. We used MaYMV coat protein (CP) gene primers to perform amplification, cloning, enzyme digestion and sequencing analysis using a positive RNA as template. The phylogenetic tree analysis showed that CP genes of Huai’an isolate and Jiangsu isolate had a close evolutionary relationship. These results indicated that this is the first report of MaYMV infection in China sorghum.

Key words: Maize yellow mosaic virus, Sorghum virus disease, Molecular detection, Phylogenetic tree analysis

Table 1

Specific primers used in this research"

病毒名称Virus name 引物名称Primer name 引物序列Primer sequence (5’-3’)
玉米黄花叶病毒Maize yellow mosaic virus MaYMV（280bp F） CAAAGGACTCTCTCACGGGC
MaYMV（280bp R） CCTCCGATTTGATTGGACGC
玉米相关单份体病毒1 Maize-associated totivirus 1 MATV（567bp F） AGTGTTAAATATGAGTGGGC
MATV（567bp R） AGCCATCCGATGATGTATCTT
玉米条纹病毒Maize stripe tenuivirus MSpV（303bp F） CGAATGATGCGAGCAAGGC
MSpV（303bp R） CACTTGACAGCTCATCTGCG
玉米黄矮病毒Maize yellow dwarf virus RMV MYDV（RMVCP-F） ATGAATTTGGGAGGCCG
MYDV（RMVCP-R） CTATTTCGGATTTTGGGTATGAA
玉米矮花叶病毒Maize dwarf mosaic virus MDMV（454bp F） TATCGAACACCCGAGCAACC
MDMV（454bp R） CGTGTGTTTGAACCACGGAC
高粱花叶病毒Sorghum mosaic virus SrMV（430bp F） AAGCTCAACGAGACGCAGAA
SrMV（430bp R） ATGAGCCCACTTGCGACTAC
高粱绿斑病毒Sorghum chlorotic spot virus SrCMV（276bp F） GCAGTTCGCCATGCGTTTAT
SrCMV（276bp R） CGCTTTCAACCCTTCTCCCT
甘蔗条纹花叶病毒Sugarcane streak mosaic virus SCSMV（575bp F） CGGTCGATGGACAAGGAACA
SCSMV（575bp R） ACGAACCGAGAACTGAACCC
甘蔗花叶病毒Sugarcane mosaic virus SCMV（433bp F） CAGGGGGCCAAAGAGACAAA
SCMV（433bp R） TACGCTTCAGCTGCATCACT
约翰草花叶病毒Johnsongrass mosaic virus JGMV（703bp F） CACCCGCTGCAAATCAAACA
JGMV（703bp R） GGTGTGCGCGAATTGATCTC
狐尾草花叶病毒Foxtail mosaic virus FoMV（338bp F） GACGAGGAGTTGGTACGCAT
FoMV（338bp R） ACGTATGGGTCGAGAAGTGC
甘蔗黄叶病毒Sugarcane yellow leaf virus SCYLV（317bp F） ACGTAGAGCACGTGTTGGAG
SCYLV（317bp R） TCCTCTTGACGGGGAAGCTA
大麦黄矮病毒Barley yellow dwarf virus BYDV（426bp F） CAGGCGGGACTGAGGTATTC
BYDV（426bp R） TGGCCGTCATCAAACTGACA
水稻黑条矮缩病毒Rice black streaked dwarf virus RBSDV（733bp F） CATTGTCAAATCGCCCCACG
RBSDV（733bp R） TACCGCGCTCCAAGTTTGTT
南方水稻黑条矮缩病毒Southern rice black streaked dwarf virus SRBSDV（562bp F） CCCACATCGCGTCATCTCAA
SRBSDV（562bp R） TGGTCGTAACCGCCATAGTG

Fig.1

Symptoms of the virus infected sorghum in field"

Fig.2

RT-PCR detection of MaYMV in suspected diseased sorghum samples M: DNA marker; (a) Detection of sorghum samples with different virus primers; (b) Detection of suspected disease samples of sorghum."

Fig.3

The amplification, clone and verification of double digestion of Huai’an MaYMV CP M: DNA marker; (a) 1 indicates amplification of MaYMV CP gene, 2 indicates negative; (b) Verification of double enzyme digestion of CP gene cloning vector."

Fig.4

The phylogenetic tree analysis based on nucleotide sequences of Huai’an MaYMV CP"

[1] Chen S, Jiang G Z, Wu J X, et al. Characterization of a novel Polerovirus infecting maize in China. Viruses, 2016, 8(5):120.
doi: 10.3390/v8050120
[2] 陈莎.深度测序鉴定玉米病毒及感病玉米组织中小RNA分析. 杭州:浙江大学, 2015.
[3] Gonçalves M C, Godinho M, Alves-Freitas D M T, et al. First report of maize yellow mosaic virus infecting maize in Brazil. Plant Disease, 2017, 101(12):2156-2157.
[4] Bernreiter A, Teijeiro R G, Jarrin D, et al. First report of maize yellow mosaic virus infecting maize in Ecuador. New Disease Reports, 2017, 36:11.
[5] Lim S, Yoon Y, Jang Y W, et al. First report of maize yellow mosaic virus on Zea mays in South Korea. Plant Disease, 2018, 102(9):1864.
[6] Palanga E, Longue R D S, Koala M, et al. First report of maize yellow mosaic virus infecting maize in Burkina Faso. New Disease Reports, 2017, 35:26.
[7] Massawe D P, Stewart L R, Kamatenesi J, et al. Complete sequence and diversity of a maize-associated Polerovirus in East Africa. Virus Genes, 2018, 54(3):432-437.
doi: 10.1007/s11262-018-1560-5
[8] Guadie D, Abraham A, Tesfaye K, et al. First report of maize yellow mosaic virus infecting maize (Zea mays) in Ethiopia. Plant Disease, 2018, 102(5):1044.
[9] Yahaya A, Dangora D B, Alabi O J, et al. Detection and diversity of maize yellow mosaic virus infecting maize in Nigeria. Virus Disease, 2019, 30(4):538-544.
[10] Read D A, Featherstone J, Rees D J G, et al. First report of maize yellow mosaic virus (MaYMV) on maize (Zea mays) in Tanzania. Journal of Plant Pathology, 2019, 101:203.
doi: 10.1007/s42161-018-0152-5
[11] Stewart L R, Todd J, Willie K, et al. A recently discovered maize polerovirus causes leaf reddening symptoms in several maize genotypes and is transmitted by both the corn leaf aphid (Rhopalosiphum maidis) and the bird cherry-oat aphid (Rhopalosiphum padi). Plant Disease, 2020, 104(6):1589-1592.
doi: 10.1094/PDIS-09-19-2054-SC pmid: 32320337
[12] Wang F, Zhou B G, Gao Z L, et al. A new species of the genus Polerovirus causing symptoms similar to maize yellow dwarf virus-RMV of maize in China. Plant Disease, 2016, 100(7):1508.
doi: 10.1094/PDIS-11-15-1259-PDN
[13] Shi Y J, Han X Y, Li Q L, et al. First report of maize yellow mosaic virus causing maize reddening in Henan, China. Plant Disease, 2022, 106(12):3220.
[14] Sun S R, Chen J S, Yang J, et al. First report of maize yellow mosaic virus infecting sugarcane in China. Plant Disease, 2019, 103(9):2482-2483.
[15] Lim S, Yoon Y, Jang Y W, et al. First report of maize yellow mosaic virus infecting Panicum miliaceum and Sorghum bicolor in South Korea. Plant Disease, 2018, 102(3):689.
[16] Yahaya A, Rwahnih M A, Dangora D B, et al. First report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria. Plant Disease, 2017, 101(7):1335.
[17] Nithya K, VishnuVardhan J, Balasaravanan S, et al. First report of maize yellow mosaic virus (MaYMV) infecting sugarcane in India and its molecular characterization. Australasian Plant Pathology, 2021, 50:633-638.
[18] Guo M Y, Yuan X Y, Song Y L, et al. First report of maize yellow mosaic virus (MaYMV) naturally infecting wheat in China. Plant Disease, 2022, 106(10):2763.
[19] Sun S R, Chen J S, He E Q, et al. Genetic variability and molecular evolution of maize yellow mosaic virus populations from different geographic origins. Plant Disease, 2021, 105(4):896-903.
doi: 10.1094/PDIS-05-20-1013-RE
[20] Mlotshwa S, Khatri N, Todd J, et al. First report of cDNA clone- launched infection of maize plants with the polerovirus maize yellow mosaic virus (MaYMV). Virus Research, 2021, 295:198297.
[21] Gray S, Gildow F E. Luteovirus-aphid interactions. Annual Review of Phytopathology, 2003, 41:539-566.
pmid: 12730400
[22] Li X Q, Hu W L, Li Y, et al. Development of an RT-LAMP assay for the detection of maize yellow mosaic virus in maize. Journal of Virological Methods, 2022, 300:114384.
doi: 10.1016/j.jviromet.2021.114384
[23] Patton M F, Bak A, Sayre J M, et al. A polerovirus, potato leafroll virus, alters plant-vector interactions using three viral proteins. Plant Cell and Environment, 2020, 43(2);387-399.
doi: 10.1111/pce.v43.2
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