Crops ›› 2024, Vol. 40 ›› Issue (1): 233-238.doi: 10.16035/j.issn.1001-7283.2024.01.031

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Molecular Detection and Identification of Maize Yellow Mosaic Virus on Sorghum in Huai’an, Jiangsu

Wang Haitao(), Ren Chunmei, Dong Yan, Li Shuo, Cheng Zhaobang, Ji Yinghua()   

  1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences / Key Laboratory of Food Quality and Safety of Jiangsu Province/State Key Laboratory Breeding Base, Nanjing 210014, Jiangsu, China
  • Received:2022-12-16 Revised:2023-09-22 Online:2024-02-15 Published:2024-02-20
  • Contact: Ji Yinghua E-mail:wanghaitao9105@163.com;jiyinghua@jaas.ac.cn

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Abstract:

In order to understand the incidence of sorghum virus disease in Jiangsu province, the 20 suspected sorghum virus disease samples were collected from Huai’an city, and molecular detection and identification were carried out. The results showed that the bands consistent with the target fragment could be amplified by using the maize yellow mosaic virus (MaYMV) primer, and the detection rate was 80%. We used MaYMV coat protein (CP) gene primers to perform amplification, cloning, enzyme digestion and sequencing analysis using a positive RNA as template. The phylogenetic tree analysis showed that CP genes of Huai’an isolate and Jiangsu isolate had a close evolutionary relationship. These results indicated that this is the first report of MaYMV infection in China sorghum.

Key words: Maize yellow mosaic virus, Sorghum virus disease, Molecular detection, Phylogenetic tree analysis

Table 1

Specific primers used in this research"

病毒名称Virus name 引物名称Primer name 引物序列Primer sequence (5’-3’)
玉米黄花叶病毒Maize yellow mosaic virus MaYMV（280bp F） CAAAGGACTCTCTCACGGGC
MaYMV（280bp R） CCTCCGATTTGATTGGACGC
玉米相关单份体病毒1 Maize-associated totivirus 1 MATV（567bp F） AGTGTTAAATATGAGTGGGC
MATV（567bp R） AGCCATCCGATGATGTATCTT
玉米条纹病毒Maize stripe tenuivirus MSpV（303bp F） CGAATGATGCGAGCAAGGC
MSpV（303bp R） CACTTGACAGCTCATCTGCG
玉米黄矮病毒Maize yellow dwarf virus RMV MYDV（RMVCP-F） ATGAATTTGGGAGGCCG
MYDV（RMVCP-R） CTATTTCGGATTTTGGGTATGAA
玉米矮花叶病毒Maize dwarf mosaic virus MDMV（454bp F） TATCGAACACCCGAGCAACC
MDMV（454bp R） CGTGTGTTTGAACCACGGAC
高粱花叶病毒Sorghum mosaic virus SrMV（430bp F） AAGCTCAACGAGACGCAGAA
SrMV（430bp R） ATGAGCCCACTTGCGACTAC
高粱绿斑病毒Sorghum chlorotic spot virus SrCMV（276bp F） GCAGTTCGCCATGCGTTTAT
SrCMV（276bp R） CGCTTTCAACCCTTCTCCCT
甘蔗条纹花叶病毒Sugarcane streak mosaic virus SCSMV（575bp F） CGGTCGATGGACAAGGAACA
SCSMV（575bp R） ACGAACCGAGAACTGAACCC
甘蔗花叶病毒Sugarcane mosaic virus SCMV（433bp F） CAGGGGGCCAAAGAGACAAA
SCMV（433bp R） TACGCTTCAGCTGCATCACT
约翰草花叶病毒Johnsongrass mosaic virus JGMV（703bp F） CACCCGCTGCAAATCAAACA
JGMV（703bp R） GGTGTGCGCGAATTGATCTC
狐尾草花叶病毒Foxtail mosaic virus FoMV（338bp F） GACGAGGAGTTGGTACGCAT
FoMV（338bp R） ACGTATGGGTCGAGAAGTGC
甘蔗黄叶病毒Sugarcane yellow leaf virus SCYLV（317bp F） ACGTAGAGCACGTGTTGGAG
SCYLV（317bp R） TCCTCTTGACGGGGAAGCTA
大麦黄矮病毒Barley yellow dwarf virus BYDV（426bp F） CAGGCGGGACTGAGGTATTC
BYDV（426bp R） TGGCCGTCATCAAACTGACA
水稻黑条矮缩病毒Rice black streaked dwarf virus RBSDV（733bp F） CATTGTCAAATCGCCCCACG
RBSDV（733bp R） TACCGCGCTCCAAGTTTGTT
南方水稻黑条矮缩病毒Southern rice black streaked dwarf virus SRBSDV（562bp F） CCCACATCGCGTCATCTCAA
SRBSDV（562bp R） TGGTCGTAACCGCCATAGTG

Fig.1

Symptoms of the virus infected sorghum in field"

Fig.2

RT-PCR detection of MaYMV in suspected diseased sorghum samples M: DNA marker; (a) Detection of sorghum samples with different virus primers; (b) Detection of suspected disease samples of sorghum."

Fig.3

The amplification, clone and verification of double digestion of Huai’an MaYMV CP M: DNA marker; (a) 1 indicates amplification of MaYMV CP gene, 2 indicates negative; (b) Verification of double enzyme digestion of CP gene cloning vector."

Fig.4

The phylogenetic tree analysis based on nucleotide sequences of Huai’an MaYMV CP"

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