Crops ›› 2025, Vol. 41 ›› Issue (5): 221-232.doi: 10.16035/j.issn.1001-7283.2025.05.029

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The Effects of Different Rotation Patterns on the Structure and Function of Fungal Communities in Potato Rhizosphere Soil in High-Altitude Black Soil Areas

Wang Ziyi1(), Han Wei2, Jin Hongbiao3, Huang Xiumei1(), Zhang Fenglan4, Yang Zhongren4   

  1. 1 Vocational and Technical College of Inner Mongolia Agricultural University, Baotou 014109, Inner Mongolia, China
    2 Xuechuan Agricultural Group Co., Ltd., Zhangjiakou 075000, Hebei, China
    3 Hulun Buir Agricultural and Animal Husbandry Science Research Institute, Hulun Buir 021000, Inner Mongolia, China
    4 College of Horticulture and Plant Protection of Inner Mongolia Agricultural University, Hohhot 010000, Inner Mongolia, China
  • Received:2024-04-09 Revised:2024-07-20 Online:2025-10-15 Published:2025-10-21

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Abstract:

In order to clarify the differences in rhizosphere soil fungal communities under different rotation patterns of potato, Illumina NovaSeq 6000 high-throughput sequencing of fungal ITS regions was used to comparatively analyse the changes in soil fungal community structure and function of six local traditional cropping patterns by the FUNGuild (Fungal Function Prediction Tool). The results showed that soil samples from the six treatments achieved 3350 ASV, classified into seven phyla, 22 classes, 72 orders, 146 families, 300 genera, and 598 species; compared with CK, T3, T4 treatment would increase the diversity and richness of soil fungi, T4 treatment had the highest diversity and abundance of soil fungi in rhizosphere; the structural composition of soil fungi at the phylum level was similar in different crop rotation patterns, but the relative abundance varied. Ascomycota was the dominant phylum, with relative abundance ranging from 58.60% to 82.66%; the PCoA analysis revealed that PC1 and PC2 could explain 38.76% of the differences in community composition; the LDA (threshold 4.0) results of LEfSe showed that 24 differential species were found at all taxonomic levels of the fungal community; the FUNGuild function comparison revealed that the main trophic types were the same as the pathotrophic and saprotrophic trophic types among the different treatments, but there were differences in the relative abundance. T2, T3 and T4 treatments were dominated by saprotroph, T3 treatment had the highest relative abundance of saprotroph, while T1 and T5 treatment were dominated by pathotroph. CK was dominated by saprotrophs with pathotrophs as the secondary trophic type. In conclusion, different rotation patterns altered the structure, diversity and trophic types of soil fungal communities, which can be used to regulate the soil microbiological environment of intensive planting systems, to promote the health and harmony of soil ecosystems in the high-altitude black soil area, and to improve grain yield.

Key words: High-altitude black soil area, Rotation pattern, Potato rhizosphere soil, Fungal communities, Fungal function

Table 1

Soil sample information and fungal categories under different rotation patterns"

处理Treatment 有效序列Effective sequences 门Phylum 纲Class 目Order 科Family 属Genus 种Species
T1 277 136 7 15 34 53 87 122
T2 233 459 7 18 51 84 136 206
T3 246 624 7 17 47 79 134 201
T4 269 395 7 18 46 91 146 219
T5 259 567 7 17 41 67 111 171
CK 246 624 6 16 40 67 120 192
合计Total 1 572 105 7 22 72 146 300 598

Fig.1

Petal map of ASV distribution in different rotation patterns"

Fig.2

Goods-coverage index and rank-abundance curve"

Table 2

Analysis of α diversity of rhizosphere soil fungal communities in different rotation patterns"

处理Treatment ACE Chao 1 Shannon Simpson
T1 398.61 398.25 4.34 0.7758
T2 394.43 394.06 4.86 0.8725
T3 498.29 497.62 5.88 0.9351
T4 585.18 585.39 6.28 0.9501
T5 499.07 498.06 4.99 0.8640
CK 453.74 453.18 5.15 0.9068

Fig.3

NMDS analysis of soil fungal community structure under different rotation patterns"

Fig.4

Composition of soil fungal communities at the phylum and genus levels under different rotation patterns"

Fig.5

PCoA analysis of soil fungal communities under different rotation patterns"

Fig.6

Heat map of soil fungal community structure at the phylum and genus levels under different rotation patterns"

Fig.7

LEfSe difference analysis and differential species linear discriminant analysis of soil fungal communities"

Fig.8

Relative abundance of soil fungal nutrient patterns under different rotation patterns"

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