Crops ›› 2020, Vol. 36 ›› Issue (5): 98-102.doi: 10.16035/j.issn.1001-7283.2020.05.015

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Purification and In-Vitro Digestion of Maximum PBs in Maize Endosperm

Zhang Congzhuo1(), Xing Lijun2, Li Nan2, Yan Feng2, Tian Huaidong2()   

  1. 1Institute of Crop Sciences, Shanxi Agricultural University (Shanxi Academy of Agricultural Sciences),Taiyuan 030031, Shanxi, China
    2Laboratory of Plant Germplasm and Genetic Resources of Crop,College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
  • Received:2020-01-07 Revised:2020-02-26 Online:2020-10-15 Published:2020-10-12
  • Contact: Tian Huaidong E-mail:170740774@qq.com;huaidongt@sxu.edu.cn

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Abstract:

In order to reveal the diversity of nutritional values of zeins in maize germplasm resources, we optimized the extraction and in-vitro digestion of protein bodies (PBs) from the maize inbred line PC130 with the wild type character of zeins. The results showed that the amount of PBs purified with the optimized purification method was more than 50 times higher than the amount based on the reported methods; 0.5mg/mL pepsin-dosage and 75min treatment-time were determined as the conditions for the most active in-vitro digestion of endosperm PBs, by analyzing the effects of the relative factors on digestion of the purified maximum-PBs-components.

Key words: Zea mays L., Maximum PBs, Purification, In-vitro digestion

Fig.1

SDS-PAGE image of PBs components extracted with buffers containing sucroses with different concentrations M: marker; 1-8: PBs components; TP: total proteins, the same below"

Fig.2

Change in contents of PBs components extracted with buffers containing sucroses with different concentrations Different lowercase letters indicate singificance at 0.05%, the same below"

Fig.3

SDS-PAGE image of PBs components extracted under optimal sucrose concentrations and precipitated by adding equivalent water and incubating for different time 1-5: PBs components"

Fig.4

Change in contents of PBs components extracted under optimal sucrose concentrations and precipitated by adding equivalent water and incubating for different time"

Fig.5

SDS-PAGE image of residues after in vitro digestion of maximum PBs components using pepsins with different concentrations 1-6: PBs components"

Fig.6

Change in digestibilities of maximum PBs components digested in vitro using pepsins with different concentrations"

Fig.7

SDS-PAGE image of residues obtained after in vitro digestion of maximum PBs components in different times under the optimum pepsin dosage 1-8: PBs components"

Fig.8

Change in digestibilities of maximum PBs components digested in vitro for different time under the optimal pepsin dosage"

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