Crops ›› 2016, Vol. 32 ›› Issue (5): 160-166.doi: 10.16035/j.issn.1001-7283.2016.05.028

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Comparison of Isolation Efficiency of Phytophthora infestans with Two Antibiotics Formulas

Wang Beibei1,Jiang Jizhi1,Li Ying1,Wan Anqi1,Gui Chunshuang1,Huang Yingju1,Yang Zhihui2,Zhu Jiehua2   

  1. 1 College of Life Sciences,Hebei University,Baoding 071002,Hebei,China
    2 College of Plant Protection,Agricultural University of Hebei,Baoding 071001,Hebei,China
  • Received:2016-06-28 Revised:2016-08-24 Online:2016-10-15 Published:2018-08-26
  • Contact: Jizhi Jiang

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Abstract:

The isolation of Phytophthora infestans strains from diseased leaves of potato in field and from the media of contaminating microorganism in lab is difficult with traditional method. The purpose of this experiment is to compare the isolation efficiency of P. infestans by Nystatin formula and Merbromin formula in rye culture medium. The results showed that purification rates of Nystatin formula and Merbromin formula was 74% and 52% from diseased potato leaves in field, respectively, but that were 36% and 78% from the media of contaminating microorganism in lab, respectively. The two formulas had some inhibition on mycelia growth of P. infestans and Merbromin formula displayed stronger inhibition in comparison with Nystatin formula, the inhibition rates were 65.79% and 18.63%, respectively. However, the two formulations had no significant effect on spoangia formation, sporangia direct germination, and zoospores release, as well as the mycelia protein content of P.infestans. In addition, both Nystatin formula and Merbromin formula did not affect the pathogenicity of P. infestans on potato tuber slices of tested three varieties. The strains isolated from diseased potato leaves in field could be kept for 16 months in frozen storage tube of rye culture medium adding Nystatin formula. These findings indicate that Nystatin formula is suitable for isolation of P. infestans strains from field and Merbromin formula for that from lab.

Key words: Phytophthora infestans, Potato, Nystatin, Merbromin, Isolation and purification

Fig.1

Rye culture medium adding two antibiotics formulas and direct isolation of P. infestans strains in field"

Fig.2

Efficiency of isolation and purification of P. infestans strains with two antibiotics formulas from diseased leaves in field contaminating microorganism"

Table 1

Comparison of direct isolation of P.infestans strains with two antibiotics formulas in field mm"

处理Treatment 从冻存管转接致病疫霉后的培养时间Incubation time of P.infestansstrains from frozen storage tube (d)
4 5 6 7 8
制霉菌素配方Nystatin formula 44.7a/3.7a 56.3a/11.0a 69.0a/18.3a 79.3a/22.0a N/M
红药水配方Merbromin formula 25.0b/122.7b 29.3b/143.7b 35.3b/158.0b 37.0b/161.7b N/M
对照Control 42.7a/126.0b N/147.7b N/M N/M N/M

Table 2

Comparison of isolation of P.infestans strains from contaminating microorganism with two antibiotics formulas in lab mm"

处理Treatment 从污染平板上转接致病疫霉后的培养时间(d)
Incubation time of P.infestans strains from contaminating microorganism
3 4 5 6 7 8 9
制霉菌素配方Nystatin formula 9.6b/3.7b 14.1b/11.0b 19.3b/18.3b 24.5b/22.0b 36.7b/23.5b N/M N/M
红药水配方Merbromin formula 23.4a/122.7a 34.2a/143.7a 42.6a/158.0a 53.6a/161.7a 61.5a/172.2a 68.3a/178.4a N/M
对照Control 26.6a/126.0a 31.2/147.7a N/M N/M N/M N/M N/M

Fig.3

Effects of two antibiotics formulas on late growth of P. infestans"

Table 3

Effects of two antibiotics formulas on conidia production of P.infestans"

处理Treatment 每视野中的孢子囊个数Number of sporangia per field of vision 平均值Mean
制霉菌素配方Nystatin formula 31 23 38 24 28 24 20 14 15 28 24.5
红药水配方Merbromin formula 19 20 18 23 37 14 13 28 30 36 23.8
对照Control 26 38 27 11 30 35 15 32 23 42 27.9

Fig.4

Effect of two antibiotics formulas on pathogenicity of P. infestans"

Table 4

Effects of two antibiotics formulas on sporangia direct germination and zoospores release of P.infestans %"

处理
Treatment		 游动孢子释放率
Zoospores release rate		 抑制率
Suppression rate		 孢子囊直接萌发率
Sporangia direct germination rate		 抑制率
Suppression rate
制霉菌素配方Nystatin formula 48.75a 3.79 45.73a 12.06
红药水配方Merbromin formula 46.58a 8.07 46.39a 10.79
对照Control 50.67 - 52.00 -

Fig.5

Standard curve of protein content in potato tuber slices"

Fig.6

Effects of different processing mode on protein content of P.infestans mycelia"

[1] Chmielarz M, Sobkowiak S , et al. Diversity of Phytophthora infestans from Poland. Plant Pathology, 2014,63(1):203-211.
doi: 10.1111/ppa.12076
[2] Arora R K, Sharma S, Singh B P . Late blight disease of potato and its management. Potato Journal, 2014,41(1):16-40.
[3] Cespedes M C, Cardenas M E, Vargas A M , et al. Physiological and molecular characterization of Phytophthora infestans isolates from the Central Colombian Andean Region. Revista Iberoamericana de Micología, 2013,30(2):81-87.
doi: 10.1016/j.riam.2012.09.005
[4] Blandon-Diaz J U, Widmark A K, Hannukkala A , et al. Phenotypic variation within a clonal lineage of Phytophthora infestans infecting both tomato and potato in Nicaragua. Phytopathology, 2012,102(3):323-330.
doi: 10.1094/PHYTO-02-11-0033
[5] 桂春爽 . 北方四省致病疫霉致病型及拮抗菌复合发酵抑菌作用的研究. 保定:河北大学, 2010.
[6] 黄英菊 . 北方五省区致病疫霉生理小种分布及3种拮抗菌的比较研究. 保定:河北大学, 2015.
[7] 刘红梅, 孟晓营, 刘兆明 , 等. A5295激发子诱导马铃薯抗晚疫病条件的优化. 作物杂志, 2015(1):67-71.
doi: 10.16035/j.issn.1001-7283.2015.01.014
[8] 蒋继志, 孙琳琳, 郭会婧 , 等. 几种微生物提取物诱导马铃薯抗晚疫病及机理的初步研究. 植物病理学报, 2010,40(2):173-179.
[9] 赵偲 . 北方五省区致病疫霉抗药性监测及拮抗细菌ST-1的初步探索. 保定:河北大学, 2015.
[10] 宋伯符, 谢开云 . CIP的全球晚疫病防治倡议与我国的参与. 马铃薯杂志, 1997,11(1):51-55.
[11] 李惠霞, 刘永刚, 王蒂 , 等. 马铃薯晚疫病菌培养条件的研究. 中国马铃薯, 2007,21(4):203-205.
[12] 王美琴, 田亚丽 . 番茄晚疫病菌的分离及对杀菌剂的毒力测定. 农业技术与装备, 2011(3):67-68,70.
[13] 包海银 . 马铃薯晚疫病菌的分离纯化和单孢分离[EB/OL]. 北京:中国科技论文在线 [2006-06-30]..
[14] 朱杰华, 张志铭, 杨志辉 . 马铃薯晚疫病菌的常规研究方法. 河北农业大学学报, 2001,24(2):112-114.
[15] 李新新 . 马铃薯晚疫病菌的长期保存与特异性SCAR标记的开发. 哈尔滨:东北农业大学, 2012.
[16] 张珏锋, 何月平, 陈建明 . 制霉菌素对褐飞虱取食行为、营养及共生菌数量的影响. 植物保护学报, 2015,42(2):264-270.
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