Crops ›› 2025, Vol. 41 ›› Issue (4): 95-103.doi: 10.16035/j.issn.1001-7283.2025.04.012

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Analysis of the Transcriptome and Jasmonic Acid Signal Transduction in Chewing Cane Leaves under Cold Stress

Chen Minghui1(), Xu Yu2, Huang Zhiqiang2, Wang Junqing1, Zhang Baoqing3()   

  1. 1School of Chemistry and Environmental Engineering, Pingdingshan University / Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan 467000, Henan, China
    2Pingdingshan Forestry Bureau, Pingdingshan 467000, Henan, China
    3Guangxi Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Nanning 530007, Guangxi, China
  • Received:2024-04-22 Revised:2024-05-25 Online:2025-08-15 Published:2025-08-12

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Abstract:

This study investigates gene expression of chewing cane under cold stress (0 ℃), aiming to provide a theoretical basis for cold-resistant breeding, introduction, and cultivation. The high-throughput sequencing platform was used to perform transcriptome sequencing in chewing cane under control group (25 ℃) and cold stress treatment group (0 ℃). Sequencing data were analyzed to screen the key genes of jasmonic acid metabolism pathway in chewing respond to cold stress, and the expression levels of the identified important genes were verified by qRT-PCR. The results showed that after low-temperature stress treatment, a total of 321 differentially expressed genes (DEGs) were screened from the two groups of samples, among which 167 were upregulated and 154 were downregulated. Gene Ontology (GO) functional enrichment analysis showed that DEGs was significantly enriched in entries such as catalytic activity, binding, metabolic processes, cellular processes and organelles. KEGG pathway enrichment analysis showed that cutin and suberine biosynthesis, photosynthesis, fatty acid metabolism, starch and sucrose metabolism, and plant hormone signal transduction were important metabolic pathways in response to cold stress in chewing cane. qRT-PCR analysis showed that genes related to the jasmonic acid metabolic pathway (SoPYL1, SoOPCL1, SoAOC1, SoLOX3, SoAOS1, and SoMYC2) were all upregulated after cold stress in chewing cane. This result is similar to the trend of transcriptome sequencing changes and further confirms the accuracy of RNA-seq data.

Key words: Chewing cane, Transcriptome sequencing, Cold stress, Jasmonic acid metabolism pathway

Table 1

qRT-PCR primer sequence"

基因ID Gene ID 基因名称Gene name 正向引物Forward primer (5'-3') 反向引物Reverse primer (5'-3')
TRINITY_DN22868_c0_g1 SoPYL1 CTGCTGTTTATTGGCAGGGC CGAGAGGCACCAAGTGGATT
TRINITY_DN34725_c1_g1 SoOPCL1 TTGTCCCAGGCTATCCCCAGTC GGCTGATAAGAGAAAGCAACGC
TRINITY_DN6267_c0_g1 SoAOC1 CCGGTGGCAAATCAGGTATGT TGTCAGTCCAAGGAGCGTACCT
TRINITY_DN13714_c0_g1 SoLOX3 AGGCAGGGCTCTTTGACATTAC TCTGACCACTCCAGCCATAGCC
TRINITY_DN23021_c3_g1 SoAOS1 GAGTTTGTGCCTGATAGATTCGT GCTTGTTACTTACTGTCGGGCT
TRINITY_DN19998_c0_g1 SoMYC2 GTGTGTGTAGAGCGTGGTTGAT TGGAGGTGTAACCAGATGCTAT
actin 内参基因 TTACGGAAACATCGTCCTCAG GAATAGACCCTCCAATCCAAAC

Table 2

The transcriptome sequencing data and quality check"

样品名称
Sample name		 读取数量
Reads count		 碱基数量
Base number		 GC含量
GC content (%)		 Q30
(%)
CK-1 24 115 557 6 616 823 305 45.38 93.75
CK-2 23 965 241 6 941 153 971 45.25 94.93
CK-3 24 120 417 7 015 779 736 45.61 93.96
LT-1 23 413 153 6 994 627 319 44.89 94.67
LT-2 24 179 352 6 631 567 115 44.76 94.33
LT-3 23 956 726 6 972 364 824 45.37 93.84

Fig.1

Classification analysis of differentially expressed genes (DEGs) (a) Gene ontology (GO) classification analysis; (b) DEGs MA map; (c) DEGs correlated clustering heatmap, red indicates high expression level, blue indicates low expression level."

Fig.2

Number of Unigene annotated to COG database"

Fig.3

The KEGG enrichment analysis of differentially expressed genes"

Fig.4

Transcriptomic expression level and qRT-PCR validation of differentially expressed genes Different lowercase letters indicate significant differences (P < 0.05)."

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