Fig.1

Electrophoretogram of Nt14-3-3-like C PCR products M: DL2000 DNA marker, the same below; 1-3: PCR products"

Fig.2

Comparison of sequencing and alignment results Nt14-3-3-like C: sequence cloned; 14-3-3-like C: sequence in tobacco genome database"

Fig.3

Bioinformatics analysis of 14-3-3-like C protein a: prediction of transmembrane structure; b: prediction of signal peptide; c: prediction of phosphorylation sites; d: prediction of protein tertiary dimensional structure"

Fig.4

Phylogenetic tree analysis of 14-3-3-like C"

Fig.5

Electrophoretogram of recombinant expression vector 35S::14-3-3-like C-GFP PCR amplification 1-3: PCR products;“-”represents negative control"

Fig.6

Subcellular localization of 14-3-3-like C The green fluorescence is 14-3-3-like C-GFP; the red fluorescence is ARR-mcherry (ARR is a nuclear marker protein)"

Fig.7

Identification of Nt14-3-3-like C expression vector 1-3: bacterium solution PCR products (a); 1-2: agarose gel electrophoresis results of restriction enzyme digestion products (b)"

Fig.8

Agrobacterium-mediated genetic transformation"

Fig.9

Detection results of transgenic plants 1-6: agarose gel electrophoresis of PCR products"

Fig.10

Detection of gene expression inhibition at transcription level “**”indicates significant different at 0.01 level, the same below. CK: control line; L1-L3: RNAi lines"

Fig.11

Gene expression analysis of pathogen defense and photosynthetic pathway"