Crops ›› 2020, Vol. 36 ›› Issue (5): 48-52.doi: 10.16035/j.issn.1001-7283.2020.05.007

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The Study of Novel Haploid Inducer with Yellow-Green Marker in Early Spontaneous Doubled Haploid Line Selection in Maize Breeding

Guo Qi1,2(), Yu Mingyan1, Ren Jun1, Dai Yuxian1, Li Shuhua1, Liu Xiaodan1, Xu Guoliang1(), Cai Zhuo1()   

  1. 1Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin, China
    2Syngenta Biotechnology (China) Co., Ltd., Beijing 102200, China
  • Received:2020-04-12 Revised:2020-04-24 Online:2020-10-15 Published:2020-10-12
  • Contact: Xu Guoliang,Cai Zhuo E-mail:gq19870405@163.com;ymxugl@163.com;caizhuo@126.com

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Abstract:

This paper disclose a novel maize haploid inducer. This inducer with kernel marker, vegetative marker and plant marker to identify haploid plants. It can indentify heterodiploid plants by yellow-green seedling marker at seedling stage. It can be widely used in EDH (early spontaneous doubled haploid), high spontaneous doubling, haploid backcross and haploid recurrent selection technology in maize breeding scheme.

Key words: Yellow-green seedling marker, EDH line, Haploid, Induction line

Table 1

Testing basic materials and sources"

材料Material 基础群体Basic population 来源Source
P1 吉C7×良S127 自组群体
P2 PH25×四287 自组群体
P3 LH82×D22 自组群体
P4 Ys517×Ys335 自组群体
P5 京科糯928 北京市农林科学院
P6 一口三香 山东登海种业股份有限公司

Table 2

The DH line and EDH line selection of yellow-green inducers inducing different populations in 2015"

诱导单倍体群体
Induced haploid population		 总粒数
Total number of grains		 准单倍体粒数
Quasi-haploid number of grains		 粗诱导率
Crude induction rate (%)		 成苗数
Number of seedlings		 黄绿苗数
Number of yellow-green seedlings		 黄绿苗预留率
Reserve rate of yellow-green seedlings (%)		 诱导率
Induction
rate (%)		 DH系
DH line		 EDH系
EDH line
P1×Jhy7-4 357 23 6.44 16 2 12.50 5.88 1 0
P1×Jhy7-8 165 18 10.91 15 2 13.33 9.70 1 0
P1xJhy7-9 232 16 6.90 14 0 0. 6.90 0 0
P1×Jhy7-11 344 22 6.40 17 4 23.53 5.23 1 0
P1×Jhy7-13 408 18 4.41 8 5 62.50 3.19 0 0
P1×Jhy7-15 410 18 4.39 11 5 45.45 3.17 0 0
P1×Jhy7-22 380 27 7.11 13 3 23.08 6.32 1 0
P1×Jhy7-32 494 29 5.87 16 5 31.25 4.86 1 0
P1×Jhy7-41 546 49 8.97 13 3 23.08 8.42 0 0
P1×Jhy7-53 477 16 3.35 9 1 11.11 3.14 0 0
P1×Jhy7-55 490 18 3.67 7 3 42.86 3.06 0 0
P1×Jhy7-56 535 16 2.99 9 2 22.22 2.62 0 0
P1×Jhy7-61 309 25 8.09 21 0 0. 8.09 2 0
P1×Jhy7-65 131 14 10.69 9 6 66.67 6.11 1 0
P1×Jhy7-68 287 38 13.24 27 7 25.93 10.80 3 1
P2×Jhy7-13 461 23 4.99 13 1 7.69 4.77 0 0
P2×Jhy7-22 261 25 9.58 21 7 33.33 6.90 1 0
P2×Jhy7 476 54 11.34 23 4 17.39 10.50 1 1
P2×Jhy7-36 360 29 8.06 17 4 23.53 6.94 0 0
P2×Jhy7-40 348 59 16.95 47 9 19.15 14.37 2 0
P2×Jhy7-42 534 24 4.49 19 5 26.32 3.56 0 0
P2×Jhy7-59 385 49 12.73 40 6 15.00 11.17 1 0
P2×Jhy7-70 557 30 5.39 21 4 19.05 4.67 1 0
P3×Jhy7-34 335 20 5.97 10 3 30.00 5.07 0 0
P3×Jhy7-44 102 16 15.69 11 2 18.18 13.73 0 0
P3×Jhy7-45 95 15 15.79 9 3 33.33 12.63 1 0
P3×Jhy7-69 145 27 18.62 26 4 15.38 15.86 1 0
P3×Jhy7-90 443 19 4.29 16 5 31.25 3.16 2 1
合计（平均）
Total (average)		 10 067 737 8.48* 478 105 24.75* 7.17* 21 3

Table 3

The DH (EDH) line selection results of yellow-green inducers inducing different waxycorn populations"

诱导单倍体群体
Induced haploid population		 每穗粒数
Grain number
per ear		 准单倍粒数
Quasi-haploid grain number		 伪单倍株数
Pseudohaploid number		 诱导率
Induction
rate (%)		 单倍体株
Haploid
plant		 DH穗数
DH spike number		 EDH穗数
EDH spike number
P5×Jyh7-17 168 18 0 10.71 2 1 0
P5×Jyh7-23 121 16 2 11.57 1 0 0
P5×Jyh7-24 212 20 2 8.49 0 0 0
P5×Jyh7-28 171 17 2 8.77 1 0 0
P5×Jyh7-29 148 18 0 12.16 1 0 0
P5×Jyh7 147 15 1 9.52 2 1 1
P5×Jyh7-35 193 22 3 9.84 1 0 0
P5×Jyh7-44 160 18 2 10.00 0 0 0
P5×Jyh7-47 165 19 1 10.91 1 0 0
P6×Jyh7-53 226 24 3 9.29 2 1 0
P6×Jyh7-56 213 23 2 9.86 1 0 0
P6×Jyh7-62 152 22 2 13.16 1 1 0
P6×Jyh7-70 165 19 0 11.52 2 1 0
P6×Jyh7-77 130 12 2 7.69 0 0 0
P6×Jyh7-79 148 18 0 12.16 1 1 0
P6×Jyh7-81 181 13 1 6.63 0 0 0
P6×Jyh7-85 212 20 2 8.49 2 0 0
P6×Jyh7-87 222 22 2 9.01 1 0 0
P6×Jyh7-94 164 21 2 11.59 2 1 0
P6×Jyh7-95 193 22 1 10.36 1 1 1
合计（平均）Total (average) 3 491 379 31 10.09* 22 8 2

Table 4

The DH (EDH) line selection results of yellow-green inducers and conventional inducers in inducing the P4 population"

诱导单倍体群体
Induced haploid population		 准单倍体苗数
Quasi-haploid grain number		 伪单倍体数
Pseudohaploid number		 准EDH株数
Number of quasi-EDH plants		 单倍体株数Haploid plant number 双单系数
Double single coefficient		 EDH准确率
EDH
accuracy (%)
黄绿苗
Yellow and green seedling		 粗壮苗
Strong seedling		 DH EDH
P4×Jhy7-016 114 2 0 0 112 2 0 0.00
P4×Jhy7-056 103 6 0 1 97 0 0 0.00
P4×Jhy7-088 116 3 0 0 113 2 0 0.00
P4×Jhy7-107 111 2 0 1 108 1 1 100.00
P4×Jhy7-110 93 9 0 0 84 0 0 0.00
P4×Jhy7-158 119 3 0 1 115 3 1 100.00
P4×吉高诱8 112 0 3 3 109 1 0 0.00
P4×吉高诱8 117 0 5 4 112 1 1 25.00
P4×吉高诱8 115 0 3 2 112 1 0 0.00
P4×吉高诱3 115 0 3 3 112 2 0 0.00
P4×吉高诱3 118 0 4 3 114 1 1 33.33
P4×吉高诱3 99 0 6 3 93 0 0 0.00
合计Total 1 449 27 23 22 1 399 17 4 0.00
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