Crops ›› 2021, Vol. 37 ›› Issue (3): 106-113.doi: 10.16035/j.issn.1001-7283.2021.03.016

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Effects of Combined Application of Biochar-Based Fertilizer and Trichoderma harzianum on the Qualities of Flue-Cured Tobacco and Tobacco-Growing Soil

Wang Kun1(), Wei Yuewei1, Ji Xiaoming1(), Yun Fei1, Zou Kai2(), Long Zhun2   

  1. 1College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Hunan Tobacco Company Shaoyang City Company, Shaoyang 422200, Hunan, China
  • Received:2020-10-14 Revised:2020-12-04 Online:2021-06-15 Published:2021-06-22
  • Contact: Ji Xiaoming,Zou Kai E-mail:562477408@qq.com;xiaomingji@henau.edu.cn;15740341@qq.com

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Abstract:

The effects of biochar-based fertilizer, Trichoderma harzianum, and the combination of the two on tobacco morbidity, rhizosphere soil available nutrients, soil enzyme activity, and flue-cured tobacco quality were studied using the Yunyan 87 as the research material. The results showed that biochar-based fertilizer could suppress the incidence of TMV which was 5.37% lower than conventional fertilization. The combined application of biochar-based fertilizer and Trichoderma harzianum could increase the resistance of tobacco plants to black shank and bacterial wilt, with relative control effects of 64.19% and 64.97%, respectively. Biochar-based fertilizer treatment increased soil organic matter, available K, available P, alkaline hydrolysis nitrogen contents, and sucrase and urease activities, which increased by 21.90%, 98.09%, 52.77%, 60.71%, 90.69% and 80.78%, respectively, compared with conventional treatment. Moreover, the combined application of biochar-based fertilizer and Trichoderma harzianum increased the total sugar and reducing sugar contents of tobacco leaves, the middle leaves increased by 2.52 and 3.46 percentage points while the upper leaves increased by 2.46 and 3.01 percentage points, respectively, compared with conventional fertilization; the ratio of potassium to chloride of the middle and upper leaves were increased by 16.51% and 19.24%, respectively, compared with conventional fertilization; the combined application of biochar-based fertilizer and Trichoderma harzianum could increase the contents of polyphenols and neutral aroma substances in tobacco leaves, compared with conventional fertilization treatments, the middle leaves were increased by 13.81% and 11.29% while, the upper leaves were increased by 12.04% and 8.60%, respectively. In this experiment, biochar-based fertilizer and Trichoderma harzianum combined application had the best effects on controlling tobacco diseases, improving soil nutrients, and tobacco leaf quality.

Key words: Biochar-based fertilizer, Trichoderma harzianum, Incidence rate, Soil quality, Flue-cured tobacco quality

Table 1

The amounts of base fertilizer and fertilizer nutrient input for each treatment kg/hm2"

处理
Treatment		 基肥施用量Base fertilizer application rate 哈茨木霉菌剂
Trichoderma harzianum		 基肥养分投入量
Base fertilizer nutrient input
烟草专用基肥
Special base fertilizer for tobacco		 生物饼肥
Biological fertilizer		 生物炭基肥
Biochar-based fertilizer		 氮
N		 磷
P2O5		 钾
K2O		 有机质
Organic matter
C1 975 450 0 0 138.00 114.00 401.25 461.25
C2 975 450 0 15
T1 750 450 1050 0 152.55 108.15 406.95 1183.50
T2 750 450 1050 15

Fig.1

The effects of different treatments on incidence and disease index"

Fig.2

The effects of different treatments on relative control effect"

Table 2

The effects of different treatments on the contents of mineral nutrient elements"

移栽后天数
Days after transplanting (d)		 处理
Treatment		 Ca
(g/kg)		 K
(g/kg)		 Mg
(g/kg)		 P
(g/kg)		 Cu
(mg/kg)		 Fe
(mg/kg)		 Mn
(mg/kg)		 B
(mg/kg)		 Zn
(mg/kg)
40 C1 28.47 17.01 3.71 2.79 16.71 206.14 105.40 23.48 24.94
C2 27.59 21.52 3.92 2.83 22.07 195.43 119.56 27.91 27.38
T1 33.65 21.09 4.60 3.41 29.33 275.38 132.11 31.29 29.16
T2 32.93 22.46 4.54 3.07 23.68 230.88 130.02 29.24 31.45
60 C1 26.99 16.28 3.05 2.03 15.34 180.55 89.73 20.97 20.37
C2 24.81 17.51 3.18 2.19 19.52 171.28 92.27 22.83 23.75
T1 27.06 19.15 3.87 2.88 25.76 223.36 111.64 28.22 25.10
T2 29.28 18.62 3.41 2.76 20.43 204.59 114.49 25.61 26.23
80 C1 25.12 14.65 2.57 1.37 14.03 161.02 64.19 18.45 18.33
C2 22.48 15.25 2.68 1.53 16.96 143.58 72.53 19.11 20.25
T1 25.57 16.09 3.11 1.95 20.82 190.19 99.85 23.08 22.36
T2 28.93 16.43 2.89 1.82 18.91 187.54 98.40 21.52 21.99

Table 3

Effects of different treatments on the contents of available nutrients in rhizosphere soil"

移栽后天数
Days after
Transplanting (d)		 处理
Treatment		 有机质
Organic matter
(g/kg)		 碱解氮
Alkaline hydrolysis
nitrogen (mg/kg)		 速效钾
Available K
(mg/kg)		 速效磷
Available P
(mg/kg)
40 C1 53.49c 108.01b 144.26b 39.90b
C2 58.28b 106.83b 148.46b 40.37b
T1 64.14a 154.07a 285.58a 57.28a
T2 64.67a 159.90a 285.77a 58.57a
55 C1 52.61b 120.11b 171.10b 49.88b
C2 54.92b 122.04b 175.27b 50.19b
T1 62.68a 163.37a 312.64a 73.05a
T2 64.13a 166.73a 309.63a 73.87a
70 C1 51.20d 114.53c 188.44b 53.04b
C2 55.23c 117.05c 190.67b 50.77b
T1 59.17b 152.97b 327.21a 76.15a
T2 64.03a 163.12a 329.42a 77.56a
85 C1 49.35b 119.38b 209.06b 40.72b
C2 51.02b 117.61b 207.21b 40.08b
T1 55.22a 154.23a 318.59a 61.49a
T2 54.86a 161.74a 311.90a 60.68a
100d C1 50.76b 103.61c 172.57b 37.75b
C2 50.20b 109.38c 177.56b 34.35b
T1 54.32a 157.92b 281.31a 51.11a
T2 55.87a 166.47a 284.59a 52.08a

Table 4

Effects of different treatments on enzyme activities in rhizosphere soil"

移栽后天数
Days after
transplanting (d)		 处理
Treatment		 蔗糖酶活性
Sucrase activity
[mg/(d·g)]		 脲酶活性
Urease activity
[μg/(d·g)]
40 C1 75.46b 383.41b
C2 73.05b 393.21b
T1 90.53a 594.14a
T2 88.11a 600.59a
55 C1 74.94b 414.96b
C2 75.38b 416.14b
T1 98.90a 658.47a
T2 100.35a 656.66a
70 C1 82.40b 423.51b
C2 84.87b 429.45b
T1 122.23a 750.08a
T2 122.50a 765.62a
85 C1 66.99b 411.45b
C2 67.60b 414.53b
T1 120.85a 573.42a
T2 124.93a 556.36a
100d C1 57.84b 393.76b
C2 57.22b 390.70b
T1 105.37a 550.59a
T2 109.11a 567.77a

Table 5

The effects of different treatments on the conventional chemical composition of the baked samples (C3F)"

处理
Treatment		 总糖
Total sugar
(%)		 还原糖
Reducing
sugar (%)		 烟碱
Nicotine
(%)		 钾
Potassium
(%)		 氯
Chlorine
(%)		 总氮
Total
nitrogen (%)		 钾氯比
Potassium to
chloride ratio		 糖碱比
Sugar to
alkali ratio		 氮碱比
Nitrogen to alkali ratio
C1 29.58c 25.50c 2.17a 2.17b 0.40a 1.91a 5.39b 11.78b 0.88a
C2 30.27bc 27.15b 1.89b 1.98c 0.38ab 1.73bc 5.16b 14.71a 0.91a
T1 31.12ab 27.78b 2.02ab 2.36a 0.36c 1.68c 6.58a 13.76a 0.83a
T2 32.10a 28.96a 1.96b 2.32ab 0.37bc 1.77b 6.28a 14.77a 0.91a

Table 6

The effects of different treatments on the conventional chemical composition of the baked samples (B2F)"

处理
Treatment		 总糖
Total sugar
(%)		 还原糖
Reducing
sugar (%)		 烟碱
Nicotine
(%)		 钾
Potassium
(%)		 氯
Chlorine
(%)		 总氮
Total
nitrogen (%)		 钾氯比
Potassium to
chloride ratio		 糖碱比
Sugar to
alkali ratio		 氮碱比
Nitrogen to alkali ratio
C1 23.69c 21.17b 2.78ab 2.14b 0.43a 2.18a 4.99c 7.62b 0.79ab
C2 24.45b 21.39b 2.83a 2.18ab 0.43a 2.25a 5.08c 7.56b 0.80ab
T1 26.17a 23.12a 2.75ab 2.29a 0.41a 2.25a 5.54b 8.42a 0.82a
T2 25.86a 23.74a 2.71b 2.28ab 0.38b 2.05b 5.95a 8.76a 0.76b

Table 7

Effects of different treatments on the contents of polyphenols of the baked samples (C3F) mg/g"

处理
Treatment		 绿原酸
Chlorogenic acid		 莨菪亭
Scopoletin		 芸香苷
Rutin		 总量
Total
C1 8.73c 0.16b 6.98b 15.86c
C2 9.19bc 0.19a 7.26ab 16.65b
T1 9.53b 0.16b 7.46a 17.15a
T2 10.28a 0.18a 7.58a 18.05a

Table 8

Effects of different treatments on the contents of polyphenols of the baked samples (B2F) mg/g"

处理
Treatment		 绿原酸
Chlorogenic acid		 莨菪亭
Scopoletin		 芸香苷
Rutin		 总量
Total
C1 9.00b 0.18b 8.91c 17.98b
C2 8.70b 0.20a 9.39b 18.18b
T1 9.81a 0.19a 9.53b 19.63a
T2 9.89a 0.20a 10.04a 20.01a

Table 9

Effects of different treatments on the contents of neutral aroma substances of the baked samples (C3F) μg/g"

处理
Treatment		 芳香族氨基酸类
Aromatic
amino acids		 美拉德反应产物
Maillard reaction
products		 类胡萝卜素降解产物
Carotenoid
degradation products		 类西柏烷类
Cembranes		 其他致香物质
Other aroma
substances		 新植二烯
Neophytadiene		 总量
Total
C1 8.46 17.51 76.29 40.63 3.37 636.27 782.53
C2 7.73 18.22 79.37 42.18 3.19 640.97 791.66
T1 9.04 18.36 84.18 42.76 4.02 698.89 857.25
T2 8.37 19.76 86.42 44.25 3.76 714.20 876.76

Table 10

Effects of different treatments on the contents of neutral aroma substances of the baked samples (B2F) μg/g"

处理
Treatment		 芳香族氨基酸类
Aromatic
amino acids		 美拉德反应产物
Maillard reaction products		 类胡萝卜素降解产物
Carotenoid degradation products		 类西柏烷类
Cembranes		 其他致香物质
Other aroma substances		 新植二烯
Neophytadiene		 总量
Total
C1 9.82 24.18 84.34 45.92 4.77 651.73 820.76
C2 13.32 26.29 83.88 44.53 4.11 637.84 809.97
T1 12.67 23.98 87.64 46.22 4.29 709.36 884.16
T2 15.82 26.74 89.47 48.38 5.15 705.78 891.34

Fig.3

Correlation analysis of tobacco diseases, soil quality and tobacco leaf quality "*" means P < 0.05, "**" means P < 0.01"

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