Crops ›› 2024, Vol. 40 ›› Issue (6): 237-241.doi: 10.16035/j.issn.1001-7283.2024.06.032

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Applied Research of Pseudomonas protegens KBD-3 in Crop Disease Control and Selenium-Enrichment

Cui Hong1(), Liu Qing2, Zhao Xiuzhen3, Zong Hao4(), Li Ying5, Shen Lili5, Jiao Yubing5, Wang Fenglong5, Yang Jinguang5, Yuan Lianlian5()   

  1. 1Xintai Modern Agricultural Development Service Center, Xintai 271200, Shandong, China
    2Tai’an Daiyue District Agricultural Technology Extension Center, Tai’an 271000, Shandong, China
    3Dong’e Agricultural Technology Extension Center, Dong’e 252201, Shandong, China
    4Linyi Tobacco Co., Ltd. of Shangdong Province, Linyi 276000, Shandong, China
    5Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China
  • Received:2024-03-15 Revised:2024-04-28 Online:2024-12-15 Published:2024-12-05

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Abstract:

In order to screen the microorganisms with good control effect for crop disease control and develop a safe way of selenium supplementation for the production of selenium-rich agricultural products, the disease resistance and nano-selenization of Pseudomonas protegens KBD-3 were studied. The results showed that the KBD-3 bacterial solution had good inactivation effect on tobacco mosaic virus (TMV), the inhibition rate was 95.77%, and had a good inhibition effect on Ralstonia solanacearum with the relative control effect on tobacco bacterial wilt reaching 70.89%. In addition, KBD-3 could reduce sodium selenite to nano-selenium, and the selenium content of mung bean sprouts treated with nano-selenium solution was greatly increased. These indicated that KBD-3 did not only provide a new microbial resource for crop disease control, but also could be used for selenium-rich cultivation of crops.

Key words: Pseudomonas protegens, Disease control, Selenium-enrichment, Tobacco mosaic virus, Bacterial wilt, Mung bean sprouts

Fig.1

Passivation of KBD-3 bacterial solution on TMV Left half: KBD-3 bacterial solution; Right half: CK."

Table 1

Inhibition effect of KBD-3 bacterial solution on TMV"

处理
Treatment		 枯斑数
Number of local necrotic lesions		 抑制率
Inhibition rate (%)
KBD-3 3.33±0.04b 95.77±1.05
CK 78.67±0.72a －

Fig.2

Antagonistic activity of KBD-3 bacterial solution against tobacco bacterial wilt Left: KBD-3 bacterial solution; Right: CK."

Table 2

Control effects of KBD-3 bacterial solution on tobacco bacterial wilt"

处理
Treatment		 发病率
Incidence
rate (%)		 病情指数
Disease
index		 相对防效
Relative control
effect (%)
KBD-3 40.24±0.07c 17.02±0.05c 70.89±0.12a
CK1 53.86±0.11b 30.21±0.03b 48.32±0.08b
CK2 89.31±0.14a 58.46±0.08a －
CK3 0 0 －

Fig.3

KBD-3 bacterial solution and its nano-selenium biosynthesis active bacterial solution Left: KBD-3 bacterial solution; Right: KBD-3 nano-selenium biosynthesis active bacterial solution."

Fig.4

SEM/EDS spectrum of biosynthesis of nano-selenium from strain KBD-3"

Table 3

Effects of different treatments on selenium content in mung bean sprouts"

处理
Treatment		 样品质量
Sample mass (g)		 硒含量
Selenium content (μg/g)
纳米硒溶液
Nano-selenium solution		 0.15
 45.35
CK 0.15 0.00
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