Crops ›› 2017, Vol. 33 ›› Issue (3): 146-150.doi: 10.16035/j.issn.1001-7283.2017.03.026

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Identification of SR13-2 Strain against Phytophthora infestans and Control of Late Blight on Detached Potato Tissues

Jiang Jizhi,Wang Youyou,Wang Xuening,Li Liyan,Wan Anqi,Li Ming   

  1. College of Life Sciences,Hebei University,Baoding 071002,Hebei,China
  • Received:2017-01-23 Revised:2017-04-17 Online:2017-06-15 Published:2018-08-26

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Abstract:

Bacterial SR13-2 strain which strongly inhibited mycelial growth and spore germination of Phytophthora infestans was obtained in earlier experiment in our lab. In order to further understand its biological characteristics and potential in controlling potato late blight, the taxonomic status of this stain was identified, and control efficiency on potato tuber slices and detached leaves were detected in this experiment. The results showed that SR13-2 strain was identified as Serratia rubidaea based on cultural features, thallus morphology, gram staining, spore staining, physiological and biochemical characteristics, as well as 16S rDNA sequences. The prevention efficiency and therapy efficiency of bacterial fluid of this strain on potato tuber slices were 91.83% and 83.33%, respectively, which were significantly better than treatments of Ridomil-MZ and other antagonistic bacteria. Similar results were also obtained on detached potato leaves. These results indicated that SR13-2 strain has a great potential in controlling potato late blight in future.

Key words: Antanistic bacteria, Phytophthora infestans, Identification, Potato, Disease control

Fig.1

The phylogenetic tree constructed of SR13-2 and its similar genera"

Fig.2

The phylogenetic tree constructed of SR13-2 and its similar species"

Table 1

Comparison of physiological and biochemistry characteristics of SR13-2 and its similar species"

特性Character SR13-2 Serratia rubidaea Serratia nematodiphila Serratia marcescens
精氨酸双水解酶Arginine dihydrolase - - - -
鸟氨酸脱羧酶Ornithine decarboxylase - - - +
赖氨酸脱羧酶Lysine decarboxylase - - - +
葡萄糖产气Glucose gas production + + - -
葡萄糖产酸Glucose acid production - - + +
阿拉伯糖产酸Arabinose acid production + + - -
阿东醇产酸Adonitol acid production + + - -
卫矛醇产酸Dulcitol acid production - - - -
乳糖产酸Lactose acid production + + - -
利用棉子糖Raffinose utilization + + - -
利用鸟氨酸Ornithine utilization + + - +
利用阿拉伯糖Arabinose utilization - - - +
利用木糖Xylose utilization + + - +
DNA酶DNA enzyme + + + +
4℃生长Growth at 4℃ - - - +

Table 2

Comparison of disease prevention and therapy of SR13-2 with several bacteria strains on tuber slices"

菌株
Strain		 病害预防Disease prevention 病害治疗Disease therapy
病情指数
Disease index		 效果(%)
Efficiency		 病情指数
Disease index		 效果(%)
Efficiency
SR13-2 8.17d 91.83 16.67f 83.33
S34-1 10.00cd 90.00 50.00e 50.00
SR13-1 10.00cd 90.00 91.67b 8.33
SH-9 13.10c 86.90 75.00d 25.00
YHX3 15.00c 85.00 55.00e 45.00
HT-6 16.16c 83.84 85.31c 14.69
Control-1 60.00b 40.00 82.35c 17.65
Control-2 100.00a 0.00 100.00a 0.00

Table 3

Comparison of disease prevention and therapy of SR13-2 with several bacteria strains on detached leaves"

菌株
Strain		 病害预防Disease prevention 病害治疗Disease therapy
病情指数
Disease index		 效果(%)
Efficiency		 病情指数
Disease index		 效果(%)
Efficiency
SR13-2 7.13e 92.87 14.73f 85.27
S34-1 13.60d 86.40 45.30e 54.70
SR13-1 13.30cd 86.70 87.74b 12.26
SH-9 14.52cd 85.48 66.20d 33.80
YHX3 19.70c 80.30 63.71d 36.29
HT-6 20.15c 79.85 80.00c 20.00
Control-1 58.30b 41.70 83.50de 16.50
Control-2 100.00a 0.00 100.00a 0.00
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