Crops ›› 2019, Vol. 35 ›› Issue (3): 178-184.doi: 10.16035/j.issn.1001-7283.2019.03.030

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Difference of Salt-Alkaline Tolerance of Three Rape and Its Two Relatives at Germination Stage

Fan Huiling,Bai Shengwen,Zhu Xuefeng,Li Zhenzhou,Qin Minggang,He Zhijun   

  1. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, Gansu, China
  • Received:2019-03-04 Revised:2019-04-01 Online:2019-06-15 Published:2019-06-12
  • Contact: Shengwen Bai

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Abstract:

To assess the salt-alkaline tolerance of three B. rapa (B. napus, B. campestris and B. juncea) and two relatives (E. sativa and S. alba) at germination stage,an experiment was conducted using different concentrations of salt and alkaline, including 0 (CK), 0.4%, 0.6%, 0.8%, 1.0%, 1.2% and 1.5% NaCl, and 0 (CK), 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and 0.6% NaHCO3, respectively. Germination potentiality, germination rate and germination index were tested. Our results showed that: under NaCl stress of different concentration, mean germination rate of three B. rapa and two relatives were B. juncea (71.7%)>B. napus (64.0%)>S. alba (62.4%)>B. campestris (48.4%)>E. sativa (30.6%). When the concentration of NaCl was 1.0%, the germination rate of B. juncea was the highest (91%), while that of E. sativa was the lowest (2%). Under NaHCO3 stress of different concentration, mean germination rate of three B. rapa and two relatives were S. alba (85.3%)>B. napus (84.3%)>B. campestris (59.1%)>E. sativa (42.9%)>B. juncea (34.3%). When the concentration of NaHCO3 was 0.4%, the germination rate of S. alba was the highest (95%), while that of B. juncea was the lowest (0). In summery, tolerance to NaCl stress of B. juncea was the highest, while that of B. campestris and E. sativa were relatively low; tolerance to NaHCO3 stress of S. alba and B. napus were high, while that of B. juncea was low. Therefore, to improve salinized soil in B. rape growing areas, it is better to use B. juncea in neutral saline soil, and S. alba or B. napus in alkaline salinized soil.

Key words: Rape, S. alba, E. sativa, Seed germination, Salt-alkaline tolerance

Table 1

Germination rate of rape and alian relatives seed under NaCl stress %"

NaCl浓度NaCl concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 98±0.0a 100±0.6a 100±0.5a 98±0.7a 100±0.0a
0.4 98±0.0a 82±0.1b 100±0.4a 92±0.1b 99±0.3b
0.6 94±2.2b 52±0.0c 100±0.2a 82±1.6c 96±1.1c
0.8 88±1.4c 8±0.9d 92±0.7b 35±1.2d 95±0.8d
1.0 53±2.8d 2±0.0e 48±0.1c 17±0.6e 91±0.2e
1.2 6±0.3e 0±0.0f 8±0.0d 15±0.3f 21±1.2f
1.5 0±0.0f 0±0.0f 0±0.0e 0±0.0g 0±0.0g
均值Mean 62.4 30.6 64.0 48.4 71.7

Table 2

Regression relationship between NaCl concentration and seed germination rate"

材料Material 一元回归方程One dimension regression equation R2 X实测区间Xmeasurement range 临界值Critical value (%)
白芥S. alba Y=122.65-76.645X 0.791 [0.32, 1.60] 1.60
芸芥E. sativa Y=96.187-78.056X 0.847 [0.00, 1.20] 1.23
甘蓝型油菜B. napus Y=126.09-79.028X 0.785 [0.33, 1.60] 1.59
白菜型油菜B. campestris Y=106.02-74.206X 0.873 [0.17, 1.40] 1.42
芥菜型油菜B .juncea Y=125.700-68.71X 0.671 [0.38, 1.60] 1.82

Table 3

Germination rate of rape and alian relatives seed under NaHCO3 stress %"

NaHCO3浓度NaHCO3concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 98±0.0b 100±0.0a 100±0.0a 98±0.0a 100±0.0a
0.1 99±0.0a 96±0.9b 98±0.3b 87±1.1b 96±0.2b
0.2 99±0.0a 58±0.1c 98±0.0b 72±0.4c 43±1.2c
0.3 99±0.3a 28±0.4d 96±1.1c 68±0.2d 1±0.0d
0.4 95±0.1c 12±0.0e 88±1.4d 47±1.1e 0±0.0e
0.5 73±0.5d 6±0.7f 68±0.9e 22±0.6f 0±0.0e
0.6 34±0.3e 0±0.0g 42±0.8f 20±0.2g 0±0.0e
均值Mean 85.3 42.9 84.3 59.1 34.3

Table 4

Regression relationship between NaHCO3 concentration and seed germination rate"

材料Material 一元回归方程One dimension regression equation R2 X实测区间Xmeasurement range 临界值Critical value (%)
白芥S. alba Y=111.86-88.571X 0.610 [0.16, 0.60] 1.26
芸芥E. sativa Y=99.214-187.86X 0.925 [0.00, 0.52] 0.52
甘蓝型油菜B. napus Y=110.43-87.143X 0.752 [0.12, 0.60] 1.26
白菜型油菜B. campestris Y=98.500-133.57X 0.957 [0.00, 0.58] 0.73
芥菜型油菜B. juncea Y=91.607-191.07X 0.796 [0.00, 0.47] 0.48

Table 5

Germination potential of rape and alian relatives seed under NaCl stress %"

NaCl浓度NaCl concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 98A 100A 100A 83A 100A
0.4 96A 82B 100A 80A 98A
0.6 94A 52C 100A 73B 96A
0.8 88B 8D 88B 22C 95B
1.0 50C 2E 46C 16D 91B
1.2 40D 0F 8D 15D 15C
1.5 0E 0F 0E 0E 0D
均值Mean 66.6 34.9 63.1 41.3 70.7

Table 6

Germination potential of rape and alian relatives seed under NaHCO3 stress"

NaHCO3浓度NaHCO3concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 98A 100A 100A 83A 100A
0.1 99A 94B 98A 83A 96B
0.2 99A 56C 98A 70B 43C
0.3 99A 16D 94B 63C 1D
0.4 95B 2E 82C 45D 0D
0.5 73C 2E 68D 22E 0D
0.6 34D 0E 42E 15F 0D
均值Mean 85 39 83 54 34

Table 7

Germination index of rape and alian relatives seed under NaCl stress"

NaCl浓度NaCl concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 85.05A 99.05A 93.17A 39.15A 84.83A
0.4 85.51A 73.31B 84.33B 30.86B 59.58B
0.6 82.88A 42.41C 81.17B 29.09B 57.02B
0.8 75.92B 6.37D 62.17C 10.54C 53.66C
1.0 22.45C 1.09E 34.47D 4.92D 32.59D
1.2 18.10D 0.00E 5.13E 4.53D 7.83E
1.5 0.00E 0.00E 0.00F 0.00E 2.00F
均值Mean 52.84 31.75 51.49 17.01 42.50

Table 8

Germination index of rape and alian relatives seed under NaHCO3 stress"

NaHCO3浓度NaHCO3concentration (%) 白芥S. alba 芸芥E. sativa 甘蓝型油菜B. napus 白菜型油菜B. campestris 芥菜型油菜B. juncea
0.0 96.00A 96.64A 98.00A 65.00A 90.00A
0.1 84.74B 56.01B 89.88B 36.67B 68.41B
0.2 87.19B 43.86C 94.77A 28.73C 33.32C
0.3 87.69B 13.88D 75.97C 22.31D 1.14D
0.4 84.17B 1.09E 66.63D 17.13E 0.00D
0.5 50.72C 2.11E 40.95E 8.93F 0.00D
0.6 27.83D 0.00E 28.83F 6.45F 0.00D
均值Mean 70.39 19.49 66.17 20.04 17.15
[1] 闫芳, 王勤礼, 郭有燕 , 等. NaCl胁迫对不同辣椒品种种子萌发的影响. 草业科学, 2013,30(9):1379-1385.
[2] 杨春武, 贾娜尔·阿汗, 石德成 ,等. 复杂盐碱条件对星星草种子萌发的影响. 草业学报, 2006,15(5):45-51.
[3] 曾广娟, 彭红丽, 赵美微 , 等. 8种盐生植物种子萌发期的耐盐性差异比较. 现代农业科技, 2015(20): 118, 121.
[4] 范惠玲, 许晓莎, 李鑫 , 等. 白菜型油菜-白芥属间远缘杂交亲和性及杂交一代幼苗挽救技术的研究. 甘肃农业大学学报, 2015,50(6):67-73.
[5] 范惠玲, 刘秦, 白生文 . 不同生态型芸芥有机物质对盐胁迫的响应. 中国农学通报, 2017,33(3):52-56.
[6] 裴毅, 杨雪君, 陈晓芬 , 等. 氯化钠和碳酸氢钠胁迫对白芥种子萌发的影响. 时珍国医国药, 2015,26(7):1750-1752.
[7] 黄镇, 杨瑞阁, 徐爱遐 , 等. 盐胁迫对3大类型油菜种子萌发及幼苗生长的影响. 西北农林科技大学学报(自然科学版), 2010,38(7):49-53.
[8] 龙卫华, 浦惠明, 陈松 , 等. 油菜三个栽培种发芽期耐盐性评价. 植物遗传资源学报, 2014,15(1):32-37.
[9] 许耀照, 曾秀存, 方彦 , 等. 盐碱胁迫对油菜种子萌发和根尖细胞有丝分裂的影响. 干旱地区农业研究, 2014,32(4):14-19.
[10] 陈新军, 胡茂龙, 戚存扣 , 等. 不同甘蓝型油菜品种种子萌发耐盐力研究. 江苏农业科学, 2007(4):26-29.
[11] 柴雁飞 . 盐碱混合胁迫对油菜种子萌发的胁迫效应. 甘肃联合大学学报(自然科学版), 2012(26):2-5.
[12] 蹇黎 . Na2CO3对野生油菜种子萌发的影响. 种子, 2017,36(9):1-5.
[13] 王治江, 刘自刚, 孙万仓 , 等. NaCl和Na2SO4胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析. 干旱地区农业研究, 2016,34(6):243-252.
[14] 刘自刚, 王志江, 方圆 . NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响. 中国油料作物学报, 2017,39(3):351-359.
[15] 李宏, 邓江宇, 程平 . NaCl胁迫对盐桦种子发芽和幼苗生长的影响. 新疆农业科学, 2010,47(3):472-476.
[16] 韩德梁, 韩烈保, 周晓静 . 中苜1号紫花苜蓿耐盐性研究. 种子, 2008,27(6):1-4.
[17] 金美芳, 朱晓清 . NaCl胁迫对油菜种子萌发和幼苗生长的影响. 种子, 2009,28(9):76-79.
[18] 陈培玉, 孔德政 . 盐碱胁迫对紫穗槐种子萌发的影响. 河南农业科学, 2013,42(5):150-152.
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