Crops ›› 2019, Vol. 35 ›› Issue (3): 37-41.doi: 10.16035/j.issn.1001-7283.2019.03.007

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Morphological Identification and Analysis of EMS-Induced Mutants from Ciqiao

Ma Mingchuan,Liu Longlong,Zhang Lijun,Cui Lin,Zhou Jianping   

  1. Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, Shanxi, China
  • Received:2019-01-02 Revised:2019-04-04 Online:2019-06-15 Published:2019-06-12
  • Contact: Jianping Zhou

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Abstract:

The mutant library provide the basic material of functional genomics. In this study, seeds of tartary buckwheat Ciqiao was treated by chemical mutagen Ethyl methane sulfonate (EMS). Mutation plants were screened in M2 generation in terms of morphology. Some of mutants identified from M2 plants were validated through M3 generation. The 480 mutant individuals were obtained including grain, leaf, and stem mutants, mature period were obtained with the comparison to the wild type. Some of them were related to important agronomic traits and biological characters, specifically, the mutant of polygonal grain was the first discovered and some mutants were rarely observed in natural variation, such as grain cracking. The mutant population of Ciqiao induced with EMS were ideal mutant population and they would be efficiently used in functional genome research and genetic improvement of tartary buckwheat.

Key words: Tartary buckwheat, Ethyl methane sulfonate (EMS), Mutant, Identification

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