Crops ›› 2023, Vol. 39 ›› Issue (1): 76-83.doi: 10.16035/j.issn.1001-7283.2023.01.011

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Effects of Drying Temperature on Nitrogen Transformation Mechanism of Cigar Tobacco during Discoloration Period

Jin Mingke1(), Chen Yongwei1, Wu Yongbing1, Yang Weili2, He Zhengchuan2, Zhao Mingqin1()   

  1. 1College of Tobacco Science, Henan Agricultural University/National Tobacco Physiological and Biochemical Research Center, Zhengzhou 450002, Henan, China
    2Dazhou Tobacco Company of Sichuan Province, Dazhou 635000, Sichuan, China
  • Received:2021-11-02 Revised:2022-05-16 Online:2023-02-15 Published:2023-02-22

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Abstract:

In order to explore the effects of drying temperature conditions on the nitrogen transformation mechanism of cigar tobacco leaves during discoloration period, taking Chuanxue No.1 as the test material, four temperature treatments（23°C, 25°C, 27°C and 29°C）were set, and the contents of main nitrogen compounds and the activities of key enzymes for nitrogen conversion in tobacco leaves were determined and analyzed. The results showed that, the activities of nitrate reductase and nitrite reductase in tobacco leaves increased first and then decreased with the increase of drying temperature during discoloration period. The enzyme activities of tobacco leaves treated at 23°C and 29°C were significantly decreased. With the increase of the drying temperature, the contents of nitrate and nitrite in each treatment showed a trend of first decreasing and then increasing, the contents of ammonia and total volatile alkali decreased significantly, and the protein content increased significantly, the contents of NH4+-N and amino acids in tobacco leaves treated at 27°C were significantly increased, and the content of nitrite was significantly decreased. The correlation analysis of ammonia conversion products in tobacco leaves showed that the ammonia content of each treatment had a significant negative correlation with total volatile alkali, total nitrogen and protein while having no significant correlation with NH4+-N and amino acid content. When the temperature in discoloration period was controlled at 27°C, the chemical components in tobacco leaves were more coordinated, and the physical properties and sensory quality of tobacco leaves were the best, and the treatment with 25°C took the second place. To sum up, suitable drying temperature conditions during discoloration period are beneficial to improve the quality of cigar tobacco leaves.

Key words: Cigar tobacco leaves, Drying temperature, Discoloration period, Ammonia, Nitrogen conversion

Table 1

Variation of moisture contents of tobacco leaves under different drying temperatures during discoloration period %"

处理
Treatment		 鲜烟叶
Fresh tobacco leaf		 凋萎期
Withering period		 变色期
Discoloration period		 干筋期
Dry gluten period
0d 3d 6d 9d 12d 15d 18d 23d
C1 89.03±0.84a 83.38±2.03a 81.19±1.21a 73.73±1.37a 62.36±0.93a 51.39±0.07a 41.89±5.50a 26.63±2.95a
C2 88.75±0.99a 82.88±1.63a 79.36±1.50ab 65.83±2.47b 53.55±2.42b 41.93±1.60b 35.78±1.15b 23.37±2.16a
C3 88.87±1.06a 82.57±0.75a 73.73±0.70bc 56.67±0.98c 44.92±0.41c 33.65±2.54c 27.78±1.32c 18.70±0.85b
C4 88.85±1.03a 83.13±1.77a 69.66±4.11c 51.83±1.10d 39.13±1.37d 28.81±0.38d 21.77±1.58d 15.09±0.32c

Fig.1

Changes of main nitrogen compounds in tobacco leaves under different drying temperatures during discoloration period Different letters indicate significant difference at P < 0.05 level, the same below"

Fig.2

The activities of NR and NiR under different drying temperatures during discoloration period"

Table 2

Correlation between nitrogen invertase and main nitrogen-containing compounds at different drying temperatures during discoloration period"

酶
Enzyme		 处理
Treatment		 硝酸盐
Nitrate		 亚硝酸盐
Nitrite		 氨
Ammonia		 NH4+-N 总挥发碱
Total volatile alkali		 总氮
Total nitrogen		 蛋白质
Protein		 氨基酸
Amino acid
NR C1 -0.739* -0.884** -0.867** -0.569 0.947** 0.925** 0.982** -0.541
C2 -0.704 -0.878** -0.903** -0.497 0.989** 0.959** 0.964** -0.529
C3 -0.696 -0.866** -0.861** -0.587 0.987** 0.981** 0.970** -0.591
C4 -0.769* -0.894** -0.757* -0.562 0.970** 0.949** 0.976** -0.607
NiR C1 0.762* 0.789* 0.202 0.962** -0.333 -0.260 -0.622 0.931**
C2 0.638 0.801* 0.191 0.968** -0.443 -0.363 -0.663 0.945**
C3 0.775* 0.819* 0.174 0.963** -0.525 -0.488 -0.701 0.933**
C4 0.822* 0.798* 0.080 0.942** -0.501 -0.547 -0.694 0.931**

Table 3

Correlation between moisture content and main nitrogen compounds in tobacco leaves under different drying temperatures during discoloration period"

指标
Index		 处理
Treatment		 硝酸盐
Nitrate		 亚硝酸盐
Nitrite		 氨
Ammonia		 NH4+-N 总挥发碱
Total volatile alkali		 总氮
Total nitrogen		 蛋白质
Protein		 氨基酸
Amino acid
含水率
Moisture content		 C1 -0.608 -0.721* -0.991** -0.187 0.974** 0.974** 0.825* -0.223
C2 -0.724* -0.809* -0.977** -0.328 0.980** 0.966** 0.887** -0.380
C3 -0.725* -0.841** -0.945** -0.466 0.986** 0.996** 0.913** -0.506
C4 -0.763* -0.874** -0.913** -0.381 0.988** 0.989** 0.917** -0.490

Table 4

Correlation between ammonia conversion products at different drying temperatures during discoloration period"

指标
Index		 处理
Treatment		 NH4+-N 总挥发碱
Total volatile alkali		 总氮
Total nitrogen		 蛋白质
Protein		 氨基酸
Amino acid
氨
Ammonia		 C1 0.185 -0.970** -0.963** -0.827* 0.221
C2 0.147 -0.942** -0.945** -0.799* 0.211
C3 0.181 -0.901** -0.930** -0.744* 0.245
C4 0.005 -0.862** -0.868** -0.685 0.147

Table 5

Comparison of physical properties of tobacco leaves under different drying temperatures during discoloration period"

处理
Treatment		 叶质重
Leaf weight (g/m2)		 叶厚
Leaf thickness (μm)		 拉力
Pull (N)		 含梗率
Stem ratio (%)		 平衡含水率
Equilibrium moisture content (%)
C1 22.63c 42.37c 1.27c 20.87a 23.53a
C2 24.26b 47.14b 1.41b 18.34b 22.46b
C3 25.72a 51.96a 1.58a 15.66c 18.13c
C4 22.31c 41.29d 1.12d 15.13c 14.73d

Table 6

Comparison of sensory quality of tobacco leaves under different drying temperatures during discoloration period"

处理
Treatment		 香气质(9.0)
Quality of aroma		 香气量(9.0)
Volume of aroma		 浓度(9.0)
Concentration		 劲头(9.0)
Strength		 刺激性(9.0)
Irritating		 杂气(9.0)
Offensive taste		 余味(9.0)
Aftertaste		 灰色(9.0)
Ash		 燃烧性(9.0)
Combustibility		 总分(81.0)
Total score
C1 5.7 5.4 5.3 5.5 5.9 6.0 5.2 5.1 5.2 49.3
C2 6.0 6.1 5.9 5.7 5.7 5.8 5.7 5.5 5.5 51.9
C3 6.2 6.4 6.1 5.9 5.5 5.6 6.0 5.7 5.8 53.2
C4 5.9 5.9 5.8 5.2 5.4 5.5 5.5 5.5 5.7 50.4
[1] 张倩颖, 罗诚, 李东亮, 等. 雪茄烟叶调制及发酵技术研究进展. 中国烟草学报, 2020, 26(4):1-6.
[2] 冯雨晴, 杨惠娟, 史宏志. 烟株发育过程中硝酸盐积累与调控的研究进展. 中国烟草学报, 2019, 25(2):109-120.
[3] 贾保顺, 孙祖正, 张锦中, 等. 烤烟成熟期氮代谢及其关键酶活性和相关基因表达分析. 河南农业大学学报, 2020, 54(4):559-565.
[4] 于宏晓, 赵砚棠, 徐海涛, 等. 主流烟气中的氨与烟叶中的氨、亚硝酸根、硝酸根的相关性. 烟草科技, 2012(3):60-63.
[5] 赵砚棠, 于宏晓, 陈建军, 等. 主流烟气氨释放量与烟叶中蛋白质和氨基酸含量相关性研究. 中国烟草学报, 2014, 20(3):23-29.
[6] 闫克玉, 周献礼, 王天奎, 等. 全国五产区烤烟氨含量的测定及其规律性研究. 郑州轻工业学院学报, 2005(2):1-3,10.
[7] 卢绍浩, 张嘉雯, 赵喆, 等. 晾制温度对雪茄烟叶多酚类物质代谢的影响. 西北农林科技大学学报(自然科学版), 2021, 49(3):18-26.
[8] 顾会战. 调制温湿度条件对雪茄外包皮烟叶生理变化与理化特性的影响. 郑州:河南农业大学, 2006.
[9] 范宁波, 张瑞娜, 路晓崇, 等. 雪茄烟叶晾制过程中颜色与水分含量和膜脂过氧化的关系. 中国烟草科学, 2020, 41(6):96-102.
高娅北, 钟秋, 王松峰, 等. 雪茄茄衣晾制过程中烟叶颜色和含水量变化及其相关分析. 中国烟草科学, 2019, 40(2):57-63,72.
[10] 刘博远, 赵松超, 李一凡, 等. 不同成熟度雪茄烟晾制过程碳水化合物及相关酶活性变化规律研究. 中国农业科技导报, 2021, 23(4):192-201.
doi: 10.13304/j.nykjdb.2019.0411
[11] 国家烟草专卖局. 烟草及烟草制品试样的制备和水分的测定―烘箱法:YC/T 31-1996. 北京: 中国标准出版社, 1996.
[12] 戴亚, 方力, 陆登梯, 等. 分光光度法测定烟草中的硝酸盐. 中国烟草科学, 2000(2):33-35.
[13] 王丽丽, 曹建敏, 于卫松, 等. 紫外-可见分光光度法测定烤烟中亚硝酸盐的含量. 烟草科技, 2013(1):64-67,72.
[14] 朱晓兰, 刘百战, 陈良. 用分光光度法测定烟叶中的氨. 烟草科技, 1998(3):19-21.
[15] 刘春奎, 贾琳, 王晓宾, 等. 广东烤烟总挥发碱、总挥发酸和pH的分析. 南方农业学报, 2014, 45(9):1647-1651.
[16] 李亚飞, 常栋, 冯雨晴, 等. 白肋烟烟叶氮同化能力及其对硝酸盐积累的影响. 中国烟草学报, 2019, 25(1):51-57.
[17] 时向东, 张晓娟, 王卫武, 等. 栽培密度对雪茄外包皮烟叶片化学成分和物理特性的影响. 中国烟草学报, 2005, 11(2):40-42.
[18] 宋世旭, 王佩, 周文, 等. 养护醇化时间对雪茄茄芯烟叶品质的影响. 烟草科技, 2018, 51(4):28-34.
[19] 时向东, 顾会战, 汪文杰, 等. 调制过程中温度对雪茄外包皮烟叶碳水化合物动态变化的影响. 河南农业大学学报, 2006, 40(2):133-136.
[20] 刘国顺. 烟草栽培学. 北京: 中国农业出版社, 2003.
[21] 严浩然. 晾制中白肋烟烟叶内蛋白质、氨基酸和氨的浓度变化. 烟草科技, 1981(2):45-51.
[22] 王瑞新. 烟草化学. 北京: 中国农业出版社, 2003.
[23] 李常军, 宫长荣, 周义和, 等. 烤烟烘烤过程中变黄温度对氮素代谢的影响. 中国烟草学报, 2001, 7(2):31-35.
[24] 周健飞, 武云杰, 薛刚, 等. 烟叶成熟期氮代谢酶活性、基因表达与烤烟氮素利用效率的关系. 植物营养与肥料学报, 2018, 24(3):625-632.
[25] May S K, 顾立江, 程红梅. 植物中硝酸还原酶和亚硝酸还原酶的作用. 生物技术进展, 2011, 1(3):159-164.
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