Table 1

Primer information for knockout vector construction and mutant verification"

Fig.1

The spatial structure of SRRM1-like protein (a) the secondary structure of SRRM1-like protein; (b) the domain of SRRM1-like protein; (c) the tertiary structure of SRRM1-like protein"

Fig.2

Evolutionary relationship and structural analysis of SRRM1-like protein"

Fig.3

PCR amplification of left and right arms of M-0 gene"

Fig.4

Electropherogram of pXEH20, P-L, P-LR plasmid P is the knockout vector pXEH20; P-L is the recombinant vector connecting the left arm of the gene; P-LR is the recombinant vector connecting the left and right arms of the gene"

Fig.5

Schematic diagram and verification of construction of knockout vector of M-0 gene (a): knockout vector pXEH20-LR construction and recombination diagram; (b): knockout vector verification electropherogram, track one and three are water controls, track two and four are the left and right arms of the M-0 gene, respectively"

Fig.6

Electropherogram of the verified M-0 transformant target genes Track one is the wild-type control; track two is the water control; track three, four, and five are the Hyg in mutants M9, M11, and M17; track six, seven, and eight are the gene in the mutants M9, M11, and M17; track nine is water control; track ten is wild-type control"

Fig.7

Electropherogram of the verified M-0 transformant flank sequences Track one is the wild-type control; track two is the water control; track three, four, and five are the M-0-L-H in mutants M9, M11, and M17; track six, seven, and eight are the M-0-H-R in the mutants M9, M11, and M17; track nine is water control; track ten is wild-type control"

Fig.8

Effects of gene knockout on the vegetative growth and stress adaptation of Magnaporthe grisea Different lowercase letters indicate significant difference at 0.05 level"