Crops ›› 2023, Vol. 39 ›› Issue (6): 257-260.doi: 10.16035/j.issn.1001-7283.2023.06.035

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Screening of Inducers for Sunflower Sclerotinia sclerotiorum and Application of Hypersensitive Protein

Li Hepeng1(), Zhang Yunhua2, Meng Qinglin2, Ma Ligong2, Yu Hongtao1, Li Haiyan3, Li Yichu2, Liu Jia2, Shi Fengmei2, Yang Fan2, Liu Liang2   

  1. 1Suihua Branch, Heilongjiang Academy of Agricultural Sciences, Suihua 152000, Heilongjiang, China
    2Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, Heilongjiang, China
    3College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Received:2022-04-15 Revised:2022-08-26 Online:2023-12-15 Published:2023-12-15

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Abstract:

In order to explore the effects of different plant inducers on sunflower sclerotiniose, field efficacy tests were carried out on ten inducers successively, two inducers with good control effect on sunflower sclerotiniose were selected, and the application technology of hypersensitive protein was further studied. The results showed that, 317g/ha of 3% hypersensitive protein MG applied to the flower plate twice at the budding stage and the initial flowering stage of sunflower could effectively induce sunflower resistance to Sclerotinia sclerotiorum, with a control effects of 52.7%-68.3%, which was not significantly different from chemical control (dimethachlon), so it was an ideal inducer of resistance to S.sclerotiorum. At the same time, the inducing effects of 2000g/ha dose of 0.5% chitosan and hypersensitive protein did not differ significantly. Sunflower sclerotinia can also be treated and prevented with it.

Key words: Sunflower sclerotinia, Inducer, Hypersensitive protein

Table 1

Control effects and yield increase of inducers against Sclerotinia sclerotiorum (2018)"

药剂
Fungicide		 发病率
Incidence
(%)		 病情指数
Disease
index		 平均防效
Average control
effect (%)		 小区产量
Plot yield
(kg)		 较对照增产
Yield increase compared
with CK (%)
低聚糖素Oligosaccharide 52.7±7.1 39.2abAB 18.8±35.4 3.04±0.69 132.62
95%超敏蛋白原粉95% harpin protein 39.3±4.9 26.8bAB 46.1±18.1 2.87±0.69 121.36
几丁聚糖Chitosan 45.3±0.9 30.5bAB 40.2±12.4 2.98±0.29 114.81
宁南霉素Ningnanmycin 53.3±11.1 37.7abAB 26.9±14.6 3.18±1.31 124.17
海藻甲克素Seaweed 46.0±5.3 32.8bAB 32.4±30.1 2.11±0.74 57.67
VBa植物疫苗VBa plant vaccine 60.7±12.9 44.3abAB 13.2±23.3 1.77±0.69 24.13
甲噻·链蛋白Methiadinil+plant activator protein 46.7±11.6 30.8bAB 41.6±16.9 1.94±0.71 52.38
菌核净Dimetachlone 40.7±4.4 25.0bB 50.0±13.0 3.91±0.33 230.39
清水CK 76.7±4.4 53.2aA 0.0 2.71±1.14 －

Table 2

Control effects and yield increase of inducers against Sclerotinia sclerotiorum (2019)"

药剂
Fungicide		 发病率
Incidence (%)		 病情指数
Disease index		 平均防效
Average control effect (%)		 小区产量
Plot yield (kg)		 较对照增产
Yield increase compared with CK (%)
3%超敏蛋白3% harpin protein 50.6±6.2 32.1bB 52.7±4.5aA 1.42±0.14 86.86
几丁聚糖Chitosan 42.3±11.8 32.1bB 51.9±11.8aA 1.36±0.23 77.88
维大利VdAL 78.5±2.7 44.8bAB 33.7±3.6abA 0.99±0.23 30.18
井冈霉素Jingangmycin A 57.4±13.6 45.0bAB 34.1±10.4abA 0.68±0.13 -11.10
蛇床素Cnidiadin 64.0±11.2 47.6bAB 30.1±18.3abAB 1.14±0.57 49.02
菌核净Dimetachlone 58.5±5.0 30.4bB 54.3±7.1aA 1.45±0.21 90.04
清水CK 68.6±9.6 67.4aA 0.0cB 0.76±0.19 －

Table 3

Control effects of different doses of hypersensitivity protein on sclerotinia sclerotiorum"

药剂
Fungicide		 发病率
Incidence (%)		 病情指数
Disease index		 平均防效
Average control effect (%)		 小区产量
Plot yield (kg)		 较对照增产
Yield increase compared with CK (%)
超敏蛋白
Harpin protein		 365g/hm2 39.9±7.1bB 12.7cC 70.8±6.2 5.57±0.47 46.75
317g/hm2 34.0±1.3bB 13.5cBC 68.3±2.2 5.62±1.01 48.11
267g/hm2 45.0±7.3bB 27.8bB 35.5±15.1 4.76±0.02 25.40
菌核净Dimetachlone 37.0±7.3bB 16.0cBC 62.2±7.3 4.95±0.96 30.46
清水CK 81.0±0.7aA 42.8aA 0.0 3.79±0.71 －

Table 4

Effects of different application periods on control effects"

药剂
Fungicide		 施药时期
Application period		 发病率
Incidence (%)		 病情指数
Disease index		 平均防效
Average control effect (%)		 小区产量
Plot yield (kg)		 较对照增产
Yield increase compared with CK (%)
超敏蛋白Harpin protein 种子包衣处理 71.8±7.4 56.8abAB 15.6±13.2 0.68±0.11 -10.37
苗期、中耕期 74.5±17.7 54.6abAB 20.8±24.6 1.17±0.30 52.98
现蕾期、始花期 50.6±6.2 32.1cB 52.7±4.5 1.42±0.14 86.92
超敏蛋白+磷酸二氢钾+硅酸钾
Harpin protein+KDP+potassium silicate		 65.2±7.5 47.2bcAB 30.4±5.8 0.90±0.17 18.05
菌核净Dimetachlone 58.5±5.0 30.4cB 54.3±7.1 1.45±0.21 90.12
清水CK 68.6±9.6 67.3aA 0.0 0.76±0.19 －
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doi: 10.11923/j.issn.2095-4050.cjas20191000226
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