Crops ›› 2025, Vol. 41 ›› Issue (4): 245-250.doi: 10.16035/j.issn.1001-7283.2025.04.031

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Effects of 5-Aminolevulinic Acid on Cucumber Seedling Growth and Soil Enzyme Activities under Drought Stress

Zhang Zhihan1(), Yao Jie2, Zhang Zhantian2, Bian Fuhua1, Zhang Ziran1,2, Chen Ping1,2, Chen Haining1,2(), Liu Baoyou1,2()   

  1. 1College of Life Sciences, Yantai University, Yantai 264005, Shandong, China
    2Yantai Academy of Agricultural Science, Yantai 265500, Shandong, China
  • Received:2024-04-12 Revised:2024-05-14 Online:2025-08-15 Published:2025-08-12

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Abstract:

To investigate the effects of 5-aminolevulinic acid (5-ALA) on cucumber seedling growth and soil enzyme activity under drought stress, cucumber variety “Avisite” was used as the experimental material. Under five different 5-ALA concentrations (0, 10, 25, 50 and 75 mg/L), morphological indicators, physiological activities indicators, and soil enzyme activities of cucumber seedling were measured. The results showed that three root applications of 300 mL of 25 mg/L 5-ALA at 7 d intervals could significantly promote the growth of cucumber seedling under drought stress, increase the biomass, and enhance the root activities of cucumber seedling. At the same time, the antioxidant enzyme activities and osmotic adjustment substance content of cucumber seedlings were increased, and the drought resistance of cucumber was enhanced. In addition, 5-ALA could also significantly increase soil phosphatase, nitrate reductase, sucrase and urease activity.

Key words: 5-aminolevulinic acid, Drought stress, Cucumber, Growth, Soil enzyme activity

Table 1

Effects of different concentrations of 5-ALA on the growth of cucumber seedlings under drought stress"

处理
Treatment
(mg/L)		 叶片数
Number of
leaves		 株高
Plant height
(cm)		 茎粗
Stem diameter
(mm)		 茎节数
Number of
stem nodes		 地上部鲜重
Fresh weight
of shoots (g)		 地下部鲜重
Fresh weight
of roots (g)
0 5.0±0.0b 18.53±0.45c 6.97±0.32a 5.0±0.0b 14.28±0.63c 16.58±0.89b
10 5.3±0.6ab 24.67±1.40b 7.63±0.31a 6.3±0.6a 18.69±0.94b 22.52±0.68a
25 6.0±0.0a 27.73±2.19a 7.60±0.46a 6.3±0.6a 20.32±0.49a 22.35±1.19a
50 5.3±0.6ab 20.37±1.81c 7.10±0.79a 5.3±0.6b 14.50±1.04c 14.01±0.46c
75 5.0±0.0b 15.10±1.35d 6.93±0.51a 5.0±0.0b 10.06±1.14d 8.98±0.54d
0 2.50±0.23b 0.83±0.10c 1.01±0.07c 6.51±0.40c 7.55±0.57b 41.08±5.40c
10 3.17±0.15a 1.57±0.08a 1.27±0.04a 7.32±0.44b 8.18±0.72a 47.91±5.16ab
25 3.34±0.13a 1.11±0.11b 1.10±0.03b 7.73±0.44a 8.13±0.66a 50.54±5.04a
50 2.39±0.11b 0.85±0.03c 0.97±0.04c 7.63±0.47a 7.67±0.46b 46.24±6.17b
75 1.35±0.08c 0.43±0.04d 0.90±0.05d 6.32±0.46c 6.77±0.54c 36.27±5.28d

Fig.1

Effects of different concentrations of 5-ALA on root activity of cucumber seedlings under drought stress The different lowercase letters indicate significant difference (P < 0.05). The same below."

Fig.2

Effects of different concentrations of 5-ALA on SPAD value of cucumber seedlings under drought stress"

Fig.3

Effects of different concentrations of 5-ALA on antioxidant enzyme activities of cucumber seedlings under drought stress"

Fig.4

Effects of different concentrations of 5-ALA on soil enzyme activity"

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