Crops ›› 2016, Vol. 32 ›› Issue (4): 56-61.doi: 10.16035/j.issn.1001-7283.2016.04.009

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Correlation Analysis between the Expression of Stearoyl Acyl Carrier Protein Dehydrogenase Gene and the Soluble Sugar and Fat Content of Oil Flax

Wang Shuyan2,Han Bing1,Zhou Simin2,Xu Jun3   

  1. 1 College of Life Science,Inner mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
    2 Agronomy College,Inner mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
    3 College of Grass Land,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
  • Received:2016-05-17 Revised:2016-06-23 Online:2016-08-15 Published:2018-08-26
  • Contact: Shuyan Wang

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Abstract:

To study the relationship between sad gene expression level and the content of soluble sugar, fat content of oil flax leaves and capsules of different developmental stages, three different unsaturated fatty acid content varieties were used to do research with the method of the quantitative real-time fluorescence PCR. The results showed that in oil flax leaves and capsules at different stages of development, expression patterns of sad gene in each period followed the normal distribution, and the difference is significant among varieties and among stages. The soluble sugar content in capsule balls and leaves was significantly different.According to the correlation analysis, with the expression of sad increased, the soluble sugar content decreased. The amount of fat was significantly positively correlated with the expression of sad gene. The expression of sad genes directly influenced soluble sugar, fat content and affected oil quality in oil flax.

Key words: Oil flax, Soluble sugar, Fat, Real-time PCR, Stearoyl acyl carrier protein dehydrogenase gene(sad)

Table 1

The number of varieties and stages of oil flax"

花后天数(d)
Days after flowering		 叶片Leaves 果球Capules
0 5 15 30 0 5 15 30
Linola AL1 AL2 AL3 AL4 AB1 AB2 AB3 AB4
CDC Gold BL1 BL2 BL3 BL4 BB1 BB2 BB3 BB4
内亚7号Neiya No.7 CL1 CL2 CL3 CL4 CB1 CB2 CB3 CB4

Table 2

Primer sequence and product size"

基因
Gene		 方向
Direction		 产物大小(bp)
Product size		 引物序列(5'-3')
Primer sequence
sad F 112 GGACCGCTGAAGAGAATAGG
R CCAGAGCCGATGAGATACTG
apt1 F 110 GTTTATGAATGCGCTTGTCTCA
R TAGAGCTGACCAGGACAAACA

Fig.1

RNA gel electrophoresis"

Fig.2

cDNA test of leaves and capsules in 4 stages with the primer of reference gene apt1 The control is Marker DL500,the target strip is 110bp"

Fig.3

Comparison of soluble sugar content in leaves and fruits of oil flax varieties The lowercase letters represent siginificant difference between treatments,the same below"

Fig.4

Fat content in different tissues of oil flax varieties"

Table 3

Pearson correlation analysis"

性状
Trait		 sad 可溶性糖含量
Soluble suger content		 脂肪含量
Fat content
sad 1
可溶性糖含量
Soluble suger content		 -0.285* 1
脂肪含量
Fat content		 0.350** -0.324** 1

Fig.5

The dissolution curve of reference apt1 and sad gene"

Fig.6

The relative expression of sad gene"

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