Crops ›› 2018, Vol. 34 ›› Issue (1): 102-106.doi: 10.16035/j.issn.1001-7283.2018.01.016

Previous Articles     Next Articles

Effects of Earthworm Fermentation Broth on Fruit and Vegetables Quality

Zhang Yan1,Yin Cui1,Cao Yun’e2   

  1. 1 Ningxia Wanhui Biological Environmental Protection Technology Co., Ltd, Yinchuan 750021, Ningxia, China
    2 School of Agriculture, Ningxia University, Yinchuan 750021, Ningxia, China
  • Received:2017-10-29 Revised:2017-12-21 Online:2018-02-20 Published:2018-08-24

Abstract:

In order to explore the effects of earthworm fermented broth on fruit and vegetables quality, tomato, cucumber, chili, medlar, grape, watermelon and muskmelon were taken as test materials in a field experiment. Three treatments including the control, routine chemical fertilizer, and earthworm fermentation broth were set up and Vitamin C, soluble sugar, soluble solids, soluble protein, free amino acids, glucose, fructose, sucrose, organic acids, and safe quality nitrate were anylyzed for fruit and vegetable nutrition quality. The results showed that the contents of vitamin C, soluble sugar, soluble protein, free amino acid, glucose, fructose and sucrose were significantly increased but the contents of organic acid and nitrate were significantly decreased under the treatment of earthworm fermentation broth. That meant that earthworm fermentation broth was good for the formation of nutritional quality, and improving the palatability for fruits and vegetables.

Key words: Earthworm fermentation liquid, Vitamin C, Protein, Soluble sugar, Free amino acid, Nitrate

Table 1

Agrotype and soil physical and chemical properties"

作物 Crop 土壤类型Agrotype pH 电导率
Electrical conductivity
(mS/cm)
有机质
Organic matter
(g/kg)
全氮
Total N
(g/kg)
全钾
Total K
(g/kg)
全磷
Total P
(g/kg)
有效氮
Available N
(mg/kg)
有效磷
Available P
(mg/kg)
有效钾
Available K
(mg/kg)
番茄Tomato 壤土 8.68 0.32 9.34 0.22 0.59 0.18 8.06 3.20 178.4
黄瓜Cucumber 粉砂壤土 8.21 0.25 5.02 0.34 0.46 0.58 12.58 19.82 156.2
辣椒Hot pepper 壤土 8.75 0.34 9.52 0.25 0.61 0.20 9.22 3.18 184.4
枸杞Medlar 沙质轻壤土 8.33 0.43 5.25 3.70 52.31 0.59 162.4 10.91 371.43
葡萄Grape 黏质土 8.44 0.55 9.23 4.86 58.29 0.68 34.23 15.65 423.00
西瓜 Watermelon 砂质土 8.29 0.48 4.38 0.31 0.44 0.12 5.34 2.73 145.67
甜瓜Muskmelon 砂质土 8.29 0.48 4.38 0.31 0.44 0.12 5.34 2.73 145.67

Table 2

Liquid mineral element content of earthworm fermentation broth"

全氮(g/100g)
Total N
全磷(g/100g)
Total P
全钾(g/100g)
Total K
有效氮(g/kg)
Available N
有效磷(g/kg)
Available P
有效钾(g/kg)
Available K
有机质(g/kg)
Organic matter
6.30 0.61 0.86 56.00 1.65 7.64 12.45

Table 3

Experiment design"

作物Crop 蚯蚓发酵液Earthworm fermentation (T) 常规施肥 Routine fertilization (CK2)
番茄
Tomato
开花后追施1次,施用量75kg/hm2,第一穗果核桃大小时连续追施5次,每次追施150kg/hm2 开花后追施1次,磷酸二氢铵30kg/hm2,硝酸钾45kg/hm2,尿素75kg/hm2;第一穗果核桃大小时连续追施5次,磷酸二氢铵15kg/hm2,硝酸钾45kg/hm2,尿素30kg/hm2
黄瓜
Cucumber
黄瓜开花后追施1次,施用量75kg/hm2,第2位结瓜后连续追施5次,每次追施150kg/hm2 黄瓜开花后追施1次,磷酸二氢铵7.5kg/hm2,硝酸钾45kg/hm2,尿素75kg/hm2;第2位结瓜后连续追施5次,每次追施磷酸二氢铵15kg/hm2,硝酸钾45kg/hm2,尿素30kg/hm2
辣椒
Hot pepper
苗期施用1次,施用量75kg/hm2,蕾期及开花期施用1次,施用量150kg/hm2,盛果期连续施用4次,每次施用量150kg/hm2 苗期施用1次,磷酸二氢铵30kg/hm2,硝酸钾45kg/hm2,尿素75kg/hm2;蕾期及开花期施用1次,磷酸二氢铵45kg/hm2,硝酸钾75kg/hm2,尿素75kg/hm2;盛果期连续施用4次,每次追施磷酸二氢铵30kg/hm2、硝酸钾45kg/hm2、尿素30kg/hm2
枸杞
Medlar
每次追施300kg/hm2,共追施3次
生育期内追肥3次,每次追施尿素75kg/hm2,磷酸二氢铵225kg/hm2,硫酸钾75kg/hm2
葡萄
Grape
萌芽期施150kg/hm2,幼果膨大期施300kg/hm2,转色期施450kg/hm2
萌芽前追施速效氮肥225kg/hm2,开花期追肥硼肥22.5kg/hm2,在果实迅速膨大期施复合肥225kg/hm2,在果实开始转色时施用钾肥300kg/hm2并喷施0.2%的磷酸二氢钾5次
西瓜
Watermelon
苗期追施1次,施用量75kg/hm2,花期追施2次,每次施用量75kg/hm2,结果期追施4次,每次施用量150kg/hm2 苗期追施1次,磷酸二氢铵2.22kg/hm2,硝酸钾5kg/hm2,硝酸钙8kg/hm2,尿素3kg/hm2;花期追施2次,每次追施硝酸钾5kg/hm2,硝酸钙5kg/hm2,尿素3kg/hm2;
结果期追施4次,每次施硝酸钾8kg/hm2,硝酸钙8kg/hm2
甜瓜
Muskmelon
苗期追施1次,施用量75kg/hm2,花期追施2次,每次施用量75kg/hm2,结果期追施4次,每次施用量150kg/hm2 苗期追施1次,磷酸二氢铵33.3kg/hm2,硝酸钾75kg/hm2,硝酸钙120kg/hm2,尿素
45kg/hm2;花期追施2次,每次施硝酸钾75kg/hm2,硝酸钙75kg/hm2,尿素45kg/hm2;
结果期追施4次,每次施硝酸钾120kg/hm2,硝酸钙120kg/hm2

Table 4

Vitamin C content of fruit under different treatments mg/100g"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 17.64±0.56c 10.84±0.63c 131.23±13.21c 21.24±1.11b 29.76±0.33c 3.89±0.16b 5.07±0.38b
CK2 22.46±1.12b 13.15±0.19b 145.46±11.90b 20.11±0.24b 27.89±1.21b 4.22±0.28b 4.11±0.41b
T 26.08±0.89a 21.78±0.74a 188.01±21.06a 28.16±0.92a 35.08±1.08a 6.08±0.21a 7.21±0.45a

Table 5

Total soluble sugar content of fruit under different treatments g/100g"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 4.41±1.31b 0.188±0.04a 0.52±0.04a 8.19±0.65b 16.54±2.94b 6.53±0.32b 4.26±0.36b
CK2 4.42±1.72b 0.185±0.07a 0.51±0.05a 8.43±1.32b 16.85±1.24b 6.00±0.97c 4.52±1.15b
T 5.73±1.15a 0.204±0.02a 0.63±0.04a 9.18±0.94a 17.99±2.74a 7.92±0.53a 5.86±0.76a

Table 6

Soluble protein content of fruit under different treatments mg/g"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 20.38±2.06b 5.92±1.25c 4.25±1.33c 1.53±0.19a 1.22±0.24a 0.56±0.05a 1.27±0.28b
CK2 18.73±1.87c 6.27±1.88b 4.76±1.15b 1.48±0.18a 1.29±0.17a 0.63±0.02a 1.20±0.17b
T 29.93±1.92a 6.50±1.54a 6.28±1.26a 1.84±0.22a 1.43±0.11a 0.90±0.06a 1.78±0.15a

Table 7

Free amino acid content of fruit under different treatments mg/g"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 20.34±1.81c 18.95±0.96ab 81.88±11.88c 2.60±0.11b 123.47±12.53b 34.51±4.75c 16.37±1.63c
CK2 23.18±0.51b 17.46±0.89b 83.83±12.45b 2.96±0.13b 125.23±4.44b 38.35±3.67b 18.33±1.44b
T 27.47±0.96a 20.38±1.51a 102.45±1.95a 3.55±0.21a 136.66±12.43a 47.70±4.87a 21.44±1.91a

Table 8

Organic acid content of fruit under different treatments %"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 0.49±0.04a 1.72±0.15b 0.56±0.08a 0.18±0.02a 0.32±0.08a 2.18±0.20a 2.48±0.14a
CK2 0.51±0.03a 1.98±0.11a 0.52±0.05a 0.17±0.01a 0.37±0.02a 2.22±0.28a 2.34±0.16a
T 0.44±0.03a 1.56±0.13c 0.45±0.60a 0.13±0.01b 0.29±0.05a 1.78±0.16b 1.96±9.11b

Table 9

Nitrate content of fruit under different treatments mg/100g"

处理Treatment 番茄Tomato 黄瓜Cucumber 辣椒Hot pepper 枸杞Medlar 葡萄Grape 西瓜Watermelon 甜瓜Muskmelon
CK1 16.34±1.48ab 41.12±3.89b 36.49±2.45b 62.51±5.41a 5.78±0.34a 41.86±2.51b 38.56±3.73b
CK2 16.74±1.21a 47.33±1.12a 56.38±7.67a 56.62±8.25b 5.34±0.55a 50.50±2.71a 45.34±3.12a
T 14.89±0.89b 38.37±2.37c 28.82±1.74c 48.25±4.40c 4.98±0.79a 40.21±3.67b 33.73±3.79c
[1] 李典友, 潘根兴, 向昌国 , 等. 土壤中蚯蚓资源的开发应用研究及展望. 中国农学通报, 2005,10(21):340-347.
doi: 10.3969/j.issn.1000-6850.2005.10.095
[2] 范如芹, 张晓平, 梁爱珍 , 等. 不同蚯蚓采样方法对比研究. 2012,32(13):4154-4159.
[3] 范如芹, 张晓平, 梁爱珍 , 等. 蚯蚓野外采样方法评述. 2010,6(21):1589-1595.
[4] Bartlett M, James I, Harris J , et al. Earthworm community structure on five English golf courses. Applied Soil Ecology, 2008,39(3):336-341.
doi: 10.1016/j.apsoil.2008.02.001
[5] Najar I A, Khan A B . Earthworm communities of Kashmir Valley,India. Tropical Ecology, 2011,52(2):151-162.
doi: 10.1017/S0266467410000842
[6] 黄燕, 汪春, 衣淑娟 . 液体肥料的应用现状与发展前景. 农机化研究, 2006,3(2):198-200.
doi: 10.3969/j.issn.1003-188X.2006.02.072
[7] 周倩倩 . 沼液及两种微生物肥料对枸杞生长、品质及产量的影响. 兰州:甘肃农业大学, 2012.
[8] 赵世杰 . 植物生理学实验指导. 北京: 中国农业科技出版社, 1998.
[9] 王柳, 张福墁, 魏秀菊 . 不同氮肥水平对日光温室黄瓜品质和产量的影响. 农业工程学报, 2007,23(12):225-229.
doi: 10.3321/j.issn:1002-6819.2007.12.043
[10] 李合生 . 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
[11] 阂炬, 施卫明 . 不同施氮量对太湖地区大棚蔬菜产量、氮肥利用率及品质的影响. 植物营养与肥料学报, 2009,15(1):151-157.
doi: 10.11674/zwyf.2009.0122
[12] 宋春阳, 单虎, 孙振钧 , 等. 复合蚯蚓液营养成分的分析. 国内科技简报, 1977,3(4):22-23.
[13] 曹建康, 姜微波, 赵玉梅 . 果蔬采后生理生化实验指导. 北京: 中国轻工业出版社, 2011: 34-78.
[14] 焦玉洁, 桑宇杰, 杨磊 , 等. 新鲜和腐熟紫茎泽兰对三种茄科蔬菜生理和辣椒产量品质的影响. 中国农业科学, 2016,49(5):874-884.
doi: 10.3864/j.issn.0578-1752.2016.05.007
[15] 要晓玮, 梁银丽, 曾睿 , 等. 不同有机肥对辣椒品质和产量的影响. 西北农林科技大学学报(自然科学版), 2011,39(10):157-162.
[16] 牛亚茹, 付祥峰, 邱良祝 , 等. 施用生物质炭对大棚土壤特性、黄瓜品质和根结线虫病的影响. 土壤, 2017,49(1):57-62.
doi: 10.13758/j.cnki.tr.2017.01.009
[17] 于志强, 徐永清, 李凤兰 , 等. 腐熟秸秆覆盖及EM菌发酵肥对黄瓜品质影响的研究. 作物杂志, 2015(3):104-110.
[1] Wang Jianan, Li Xiaoyan, Wei Shimei, Zhao Huijie, Zhao Mingqi, Wang Yuexia. Regulation of Exogenous 5-Aminolevulinic#br# Acid on Photosynthesis and D1 Protein of#br# Wheat Seedlings under Drought Stress [J]. Crops, 2018, 34(5): 121-126.
[2] Mingjun Zhang,Zhongfeng Li,Lili Yu,Jun Wang,Lijuan Qiu. Identification and Screening of Protein Subunit Variation Germplasm from Both Mutants and Natural Population in Soybean [J]. Crops, 2018, 34(3): 44-50.
[3] Yingjie Zhu,Xuhong Chang,Demei Wang,Yushuang Yang,Yu Wang,Bing Lü,Ruiqi Ma,Ying Liu,Yujiao Wang,Guangcai Zhao,Zhiqiang Tao. Effects of Black Soil and Alluvial Soil on Nutritional Quality of Different Spring Wheat [J]. Crops, 2017, 33(6): 84-90.
[4] Shujie Wang,Hui Feng,Zhanning Gao,Zhenggang Xue,Yongqian Yang,Zhengmao Pan,Chunsheng Zhang. Effects of Nitrogen Fertilization Rate on Grain Filling and Yield of Two Barley Varieties with Different Row Type [J]. Crops, 2017, 33(4): 129-133.
[5] Shaohui Huang,Junfang Yang,Liangliang Jia,Yunma Yang,Wenfang Yang,Yanming Sun. Effects of Slow-Release Urea Formaldehyde on Yield of Winter Wheat and the Distribution of Nitrate Nitrogen in Soil [J]. Crops, 2017, 33(3): 110-114.
[6] Yixin Tian,Fengju Gao. The Response of Growth and Dry Matter Accumulation and Distribution of High Protein Soybean to Plant Density [J]. Crops, 2017, 33(2): 121-125.
[7] Hongxin Wang,Yi Yuan,Sudan Yang,Hongmei Liu. Physiological and Biochemical Characteristics and Root Morphology of Fagopyrum megaspartanium under Hydroponic Culture [J]. Crops, 2017, 33(1): 83-87.
[8] Xiushi Yang,Zhongxian Guo,Huimin Guo,Hui Wang,Sancai Liu. Effects of Sowing Date and Seeding Density on the Yield and Quality of Buckwheat [J]. Crops, 2017, 33(1): 88-93.
[9] Ximing Xu,Xin Zhang,Lili Shi,Jing Cui,Deliang Ding,Hongyan Qu,Shouxian Gu,Yongjie Li. Evaluation of Rice Quality with Low Amylose Content in Hybrid Japonica Rice Combinations [J]. Crops, 2016, 32(6): 44-48.
[10] Shuyan Wang,Bing Han,Simin Zhou,Jun Xu. Correlation Analysis between the Expression of Stearoyl Acyl Carrier Protein Dehydrogenase Gene and the Soluble Sugar and Fat Content of Oil Flax [J]. Crops, 2016, 32(4): 56-61.
[11] Lu Wang,Zihang Cheng,Yongchao Song,Xiaonan Wang,Lianshuang Fu,Zhuofu Li,Lu Chen. Impacts of Contents of Soluble Sugars and Protein in Leaves of Winter Wheat on Spike Differentiation in Frigid Region [J]. Crops, 2016, 32(3): 99-103.
[12] Bowen Guo,Xue Zhao,Xiaoshuang Wei,Yanjing Han,Qiangfei Liang,Bo Song,Shanshan Liu. Development of a Set of 7S and 11S Multi-Subunit-Deficient Mutants with Chinese Soybean Genetic Background [J]. Crops, 2016, 32(2): 43-49.
[13] Xiaoqin Su,Hongru Wei,Shuangmei Duan,Ming Zhao,Yinghong Pan. Comparison of Preparation and Digestion for Crop Leaf Proteomes [J]. Crops, 2016, 32(2): 50-56.
[14] . [J]. Crops, 2013, 29(3): 53-55.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .