Crops ›› 2023, Vol. 39 ›› Issue (6): 127-134.doi: 10.16035/j.issn.1001-7283.2023.06.018

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Response of Soil Bacterial Community Structure and Functional Diversity to Flue-Cured Tobacco Continuous Cropping

Ao Jincheng1(), Wang Zheng2, Yang Qigang2, Li Zhi2, Wei Jianyu2()   

  1. 1College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China
    2China Tobacco Guangxi Industrial Co., Ltd., Nanning 530001, Guangxi, China
  • Received:2022-02-10 Revised:2023-06-12 Online:2023-12-15 Published:2023-12-15

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Abstract:

Exploring the effects of flue-cured tobacco continuous cropping on soil bacterial community structure and functional diversity have theoretical and guiding significance for understanding the causes of continuous cropping obstacles and formulating soil conservation measure. The 16S rDNA V3-V4 fragments of soil bacteria were sequenced by high-throughput sequencing technology in three main tobacco fields of Yunnan province with different continuous cropping years (0, 2, 4 and 8 years), and the correlation between bacterial community distribution and environmental factors were analyzed. The results showed that the cumulative sum of the relative abundance of the top 10 dominant bacteria in Qujing and Honghe were 11.43%-17.46%, and 15.82%- 22.35%, respectively. The richness and diversity of bacterial community were significantly increased with the increasing of continuous cropping years. The cumulative relative abundance of the top 10 soil dominant bacteria in Wenshan tobacco field ranged from 17.66% to 23.08%, and showed a trend of ‘first decreasing and then increasing’ with the extension of continuous cropping years. The richness and diversity of bacterial community was increased significantly with the extension of continuous cropping years. The abundances of amino acid metabolism and other secondary synthesis functions in soil bacteria community were the main metabolic pathway of continuous cropping soil bacteria, and increased with the increase of continuous cropping years, and there were differences between regions. The soil chemical properties were closely related to bacterial community distribution, and pH contributed the highest (18.12%) to soil bacterial community changes. In general, the diversity of soil bacterial community and functional diversity in different tobacco-producing area, and the relative abundance of dominant bacteria had different trends with the increase of continuous cropping years. Regulating soil pH was beneficial to maintaining a relatively stable of micro-environment of soil.

Key words: Continuous cropping, Soil health, Bacterial community evolution, Functional diversity, High- throughput sequencing

Table 1

Information on the soil samples for testing"

产区
Producing area		 样地
Sample plot		 海拔
Altitude (m)		 经度
Longtitude		 纬度
Latitude
曲靖Qujing 旧县 1925.3 103°23′10″ 25°20′20″
红河Honghe 白水 1803.9 103°52′26″ 24°40′03″
文山Wenshan 五珠 1647.5 104°31′23″ 23°48′56″

Fig.1

Abundance stacked map of soil bacterial dominant populations in continuous cropping tobacco fields at the genus level"

Fig.2

Indexes of Sobs and Shannon of continuous cropping soil bacterial community in different tobacco-planting areas The different capital and lowercase letters indicate significant differences between different treatments at 0.01 and 0.05 levels, respectively"

Fig.3

β diversity of soil bacterial community of continuous cropping tobacco fields in different tobacco-planting areas"

Fig.4

Heat map of bacterial community functional abundance of soil in continuous cropping tobacco fields"

Table 2

Characteristic of soil chemical properties of continuous cropping tobacco fields"

项目Item Q0 Q2 Q4 Q8 H0 H2 H4 H8 W0 W2 W4 W8
pH 5.7A 5.0B 5.3B 5.2B 7.1a 7.0a 6.6b 6.5b 5.1b 5.2ab 5.5a 5.6a
有机质Organic matter (g/kg） 31.4b 40.6a 44.5a 46.0a 18.4B 32.2A 37.4A 37.5A 41.0a 27.4b 33.0ab 31.6ab
碱解氮Alkaline hydrolyzed N (mg/kg) 70.0b 78.4ab 63.0b 100.8a 41.2C 76.9B 113.2A 112.0A 61.0bc 50.5c 81.0ab 100.8a
速效磷Available P (mg/kg) 9.1B 15.8B 12.4B 44.6A 3.9D 16.5C 54.3A 27.4B 16.5C 53.9A 54.8A 30.3B
速效钾Available K (mg/kg) 27.3B 44.2A 35.7A 119.6A 69.3D 320.2B 756.3A 148.5C 88.5C 267.9A 188.2A 167.7B

Fig.5

Correlation analysis of soil chemical factors and bacterial community distribution N, P, K and C represent alkali-hydrolyzable nitrogen, available phosphorus, available potassium, and organic matter, respectively.“*”indicates a significant correlation (P < 0.05);“**”and“***”indicate significant correlation at P < 0.01 and P < 0.001 levels, respectively"

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