Crops ›› 2024, Vol. 40 ›› Issue (6): 232-236.doi: 10.16035/j.issn.1001-7283.2024.06.031

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Investigation on Main Viral Diseases in Virus-Free Seed Potato in Ningxia

Li Zongze(), Lu Zhanjun(), Shi Yunyun, Du Wei, Jiang Xudong, Yang Mingjin, Yang Junli, Huang Xiuqin, Yang Fei   

  1. Ningxia Agricultural Technology Extension Station, Yinchuan 750001, Ningxia, China
  • Received:2023-11-24 Revised:2024-06-11 Online:2024-12-15 Published:2024-12-05

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Abstract:

Potato is an important food and cash crop in Ningxia, one of the primary factors influencing potato productivity and quality is potato virus disease, and the most effective method of controlling potato virus disease is detoxifying seed potato. In order to investigate the virus-carrying situation of virus-free seed potato and to clarify the occurrence trend of seed potato virus diseases in Ningxia, a total of 1105 seed potato samples, including virus-free test-tube seedlings, breeder seed, pedigree seed, first-degree species and other types of potato were collected from major virus-free seed potato production counties (districts, farms) of Ningxia from 2019 to 2023, and then all the samples were detected to determine the carrying status of the six most serious viruses (PLRV, PVY, PVA, PVS, PVM and PVX) by DAS-ELISA technology. The results showed that six kinds of potato viruses were carried in all tested potato produced in Ningxia, and there was an obvious regional difference. The detection rate of PLRV was the highest among all the virus, up to 4.16%, followed by PVY (3.98%), PVA (3.80%), PVS (3.62%) and PVM (2.17%), the PVX was the lowest, only 1.63%. The presence of a single virus (11.12%) and multiple viruses (2.97%) existed simultaneously in seed potato, but overall virus carrying rate of seed potato showed a decreasing trend year by year.

Key words: Seed potato, Virus diseases, DAS-ELISA, Prevention and control

Table 1

Sample information of virus-free seed potatoes"

编号
Number		 采样地点
Sampling location		 样品数量Sample number
2019 2020 2021 2022 2023
1 西吉县 73 120 81 145 23
2 原州区 43 106 66 53 50
3 隆德县 － 18 14 33 7
4 海原县 － 12 34 39 14
5 同心县 － 8 5 34 5
6 农垦集团 58 44 10 － －
7 彭阳县 － 2 8 － －
合计Total 174 310 218 304 99

Table 2

Virus detection results of potato seed potato samples"

病毒种类
Virus type		 带毒样品数量
Number of virus carrying samples		 侵染率
Infection rate (%)
PLRV 46 4.16
PVY 44 3.98
PVA 42 3.80
PVS 40 3.62
PVM 24 2.17
PVX 18 1.63
合计Total 156 14.12

Table 3

Detection results of six viruses in seed potato samples of different years %"

病毒种类
Virus type		 侵染率Infection rate
2019 2020 2021 2022 2023
PLRV 0.57 5.81 10.00 1.64 0.00
PVX 0.57 3.87 0.45 0.33 3.03
PVY 9.77 8.71 0.00 0.00 0.00
PVS 1.72 6.13 4.09 1.64 4.04
PVA 0.00 1.61 5.45 8.22 0.00
PVM 4.02 2.58 1.82 1.64 0.00
整体侵染率
Total infection rate		 16.65
 16.45
 14.09
 12.50
 7.07

Table 4

Statistics of poison carried by different varieties of potato in Ningxia %"

编号
Number		 采用地点
Sampling location		 样品数量
Sample number		 侵染率Infection rate 整体侵染率
Total infection rate
PVY PVX PVS PVM PVA PLRV
1 西吉县 442 3.85 2.49 3.85 3.62 0.68 2.94 12.44
2 原州区 318 5.35 1.89 3.14 0.94 5.97 10.06 16.67
3 隆德县 72 0.00 0.00 1.39 0.00 2.78 0.00 4.17
4 海原县 99 0.00 0.00 2.02 2.02 0.00 1.01 5.05
5 同心县 52 0.00 0.00 9.62 0.00 34.62 0.00 40.38
6 农垦集团 112 8.93 0.89 2.68 2.68 0.00 0.00 15.18
7 彭阳县 10 0.00 0.00 20.00 0.00 0.00 0.00 20.00

Fig.1

Statistics of virus carrying rates of different varieties potato breeding materials"

Table 5

Individual and complex virus infection rates of potato seed potatoes %"

病毒
Virus		 侵染率
Infection rate		 病毒
Virus		 侵染率
Infection rate		 病毒
Virus		 侵染率
Infection rate		 病毒
Virus		 侵染率
Infection rate
PLRV 2.44 PVA+PLRV 0.81 PVY+PVX 0.09 PVY+PVX+PVS+PLRV 0.18
PVY 2.26 PVS+PVM 0.27 PVX+PLRV 0.09 PVY+PVX+PVS+PVM 0.09
PVS 2.26 PVY+PVS 0.18 PVS+A+PLRV 0.27 PVS+PVM+PVA+PLRV 0.09
PVA 1.99 PVS+PVA 0.18 PVY+PVX+PVA 0.18 PVY+PVX+PVS+PVM+PVA 0.09
PVM 1.45 PVY+PLRV 0.09 PVY+PVX+PLRV 0.09 PVY+PVX+PVM+PVA+PLRV 0.09
PVX 0.72 PVY+PVM 0.09 PVY+PVS+PVA 0.09 － －
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