Crops ›› 2020, Vol. 36 ›› Issue (3): 197-200.doi: 10.16035/j.issn.1001-7283.2020.03.030

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Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province

Meng Feng, Zhang Yaling, Jin Xuehui()   

  1. Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control/Heilongjiang Plant Resistance Research Center, Daqing 163319, Heilongjiang, China
  • Received:2019-10-12 Revised:2020-01-21 Online:2020-06-15 Published:2020-06-10
  • Contact: Xuehui Jin E-mail:Jxh2686@163.com

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Abstract:

In order to detect the presence of the avirulence genes ACE1, AVR-Pi9, AVR-Pia, AVR-Pii and AVR1-CO39 of Magnaporthe oryzae in Heilongjiang Province, the DNA of 208 strains of Magnaporthe oryzae in Heilongjiang Province was detected by PCR using 5 pairs avirulence gene primers. The results showed that the highest frequency of ACE1 was 87.98%; the frequency of AVR1-CO39 was 0; the frequency of avirulence gene AVR-Pi9 was 79.81%; the frequency of amplification of AVR-Pia was 16.35% and the amplification frequency of AVR-Pii was 0.48%. The composition of the avirulence gene population of Magnaporthe oryzae in Heilongjiang Province is complex and has obvious regional characteristics.

Key words: Heilongjiang Province, Magnaporthe oryzae, Avirulence gene, Detection

Table 1

The primers for amplification of Magnaporthe oryzea avirulence genes"

无毒基因
Avirulence
gene		 引物序列 (5′-3′)
Primer sequence (5′-3′)		 目的片段长度
Length of targeted fragment (bp)
ACE1 F: GTGGAATCTTGTGGTGCTACGG
R: ACGGGAACATCGACGGAAAG		 1 057
AVR-Pi9 F: CGGCAGGTGGGTTTATCATC
R: CCAAAGCAGACAGCGACTCTA		 1 070
AVR-Pia F: TAGCCATTTCGGTCTCAT
R: CGGGTAAACCCTGTCATA		 1 597
AVR-Pii F: TATGCAGGCCCAAATCCGTAG
R: CCCGCAATAGTCCATAACTGTCC		 526
AVR1-CO39 F: CCTTGCCGCTACTGAAAC
R: CCCTATCGCAGAGGAACA		 419

Table 2

The frequencies of avirulence genes in Magnaporthe oryzae in Heilongjiang Province in 2017"

无毒基因
Avirulent gene		 出现个数
Number of occurrences		 出现频率
Occurrence frequency (%)
ACE1 183 87.98
AVR-Pi9 166 79.81
AVR-Pia 34 16.35
AVR-Pii 1 0.48
AVR1-CO39 0 0.00

Table 3

The distribution of avirulence genes in different regions of Heilongjiang Province"

序号
No.		 地区Area 总数
Total		 ACE1 AVR-Pi9 AVR-Pia AVR-Pii AVR1-CO39
数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency
S1 齐齐哈尔甘南 16 15 93.75 14 87.50 0 0.00 0 0.00 0 0
S2 齐齐哈尔哈拉海农场 6 5 83.33 5 83.33 4 66.67 0 0.00 0 0
S3 大庆杜蒙 19 18 94.74 13 68.42 0 0.00 0 0.00 0 0
S4 大庆肇源 11 11 100.00 11 100.00 0 0.00 0 0.00 0 0
S5 绥化望奎 7 7 100.00 7 100.00 4 57.14 0 0.00 0 0
S6 绥化庆安 14 13 92.86 13 92.86 8 57.14 0 0.00 0 0
S7 哈尔滨通河 16 12 75.00 11 68.75 3 18.75 0 0.00 0 0
S8 哈尔滨依兰 23 21 91.30 21 91.30 0 0.00 0 0.00 0 0
S9 哈尔滨方正 4 4 100.00 4 100.00 0 0.00 0 0.00 0 0
S10 哈尔滨尚志 10 10 100.00 10 100.00 1 10.00 0 0.00 0 0
S11 佳木斯桦川 10 9 90.00 5 50.00 0 0.00 0 0.00 0 0
S12 佳木斯汤原 22 15 68.18 16 72.73 8 36.36 0 0.00 0 0
S13 佳木斯桦南 15 13 86.67 13 86.67 0 0.00 0 0.00 0 0
S14 鹤岗绥滨 16 16 100.00 13 81.25 3 18.75 0 0.00 0 0
S15 鸡西虎林 9 5 55.56 5 55.56 3 33.33 0 0.00 0 0
S16 鸡西密山 10 9 90.00 5 50.00 0 0.00 1 10.00 0 0

Fig.1

The occurrence frequency of each avirulence gene in different areas of Heilongjiang Province"

[1] Couch B C, KohnL M . A multilocus gene genealogy concordant with host preference indicates segregation of a new species,Magnaporthe oryzae,from M. grisea. Mycologia, 2002,94(4):683-693.
doi: 10.1080/15572536.2003.11833196
[2] Ou S H . Rice Diseases. Kew:Commonwealth Mycological Institute, 1985.
[3] 孙强, 张三元, 张俊国 , 等. 东北水稻生产现状及对策. 北方水稻, 2010,40(2):72-74.
[4] Flor H H . Current status of the gene-for-gene concept. Annual Review of Phytopathology, 1971,9(1):275-296.
doi: 10.1146/annurev.py.09.090171.001423
[5] Marcel S, Sawers R, Oakeley E , et al. Tissue-adapted invasion strategies of the rice blast fungus Magnaporthe oryzae. The Plant Cell, 2010,22(9):3177-3187.
doi: 10.1105/tpc.110.078048
[6] Liu W D, Zhou X Y, Li G T , et al. Multiple plant surface signals are sensed by different mechanisms in the rice blast fungus for appressorium formation. PLoS Pathogens, 2011,7(1):e1001261.
doi: 10.1371/journal.ppat.1001261
[7] Ma J H, Wang L, Feng S J , et al. Identification and fine mapping of AvrPi15,a novel avirulence gene of Magnaporthe grisea. Theoretical and Applied Genetics, 2006,113(5):875-883.
doi: 10.1007/s00122-006-0347-6
[8] Sweigard J A, Carroll A M, Kang S , et al. Identification,cloning,and characterization of PWL2,a gene for host species specificity in the rice blast fungus. The Plant Cell, 1995,7(8):1221-1233.
[9] Kang S, Sweigard J A, Valent B . The PWL host specificity gene family in the blast fungus Magnaporthe grisea. Molecular Plant-Microbe Interactions, 1995,8(6):939-948.
doi: 10.1094/MPMI-8-0939
[10] Orbach M J, Farrall L, Sweigard J A , et al. A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta. The Plant Cell, 2000,12(11):2019-2032.
doi: 10.1105/tpc.12.11.2019
[11] Collemare J, Pianfetti M, Houlle A E , et al. Magnaporthe grisea avirulence gene ACE1 belongs to an infection-specific gene cluster involved in secondary metabolism. New Phytologist, 2010,179(1):196-208.
doi: 10.1111/nph.2008.179.issue-1
[12] Farman M L, Leong S A . Chromosome walking to the AVR1-CO39 avirulence gene of Magnaporthe grisea: Discrepancy between the physical and genetic maps. Genetics, 1998,150(3):1049-1058.
[13] Li W, Wang B H, Wu J , et al. The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. Molecular Plant-Microbe Interactions, 2009,22(4):411-420.
doi: 10.1094/MPMI-22-4-0411
[14] Yoshida K, Saitoh H, Fujisawa S , et al. Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. The Plant Cell, 2009,21(5):1573-1591.
doi: 10.1105/tpc.109.066324
[15] Wu J, Kou Y J, Bao J D , et al. Comparative genomics identifies the Magnaporthe oryzae avirulence effector AVR-Pi9 that triggers Pi9-mediated blast resistance in rice. New Phytologist, 2015,206(4):1463-1475.
doi: 10.1111/nph.13310
[16] Schneider D R, Saraiva A M, Azzoni A R , et al. Overexpression and purification of PWL2D,a mutant of the effector protein PWL2 from Magnaporthe grisea. Protein Expression and Purification, 2010,74(1):24-31.
doi: 10.1016/j.pep.2010.04.020
[17] Zhang S L, Wang L, Wu W H , et al. Function and evolution of Magnaporthe oryzae avirulence gene AVR-Pib responding to the rice blast resistance gene Pib. Scientific Reports, 2015,5:11642.
doi: 10.1038/srep11642
[18] 张崎峰 . 用SSR与SCAR标记对黑龙江省稻瘟病菌AVR-pit等无毒基因的检测. 大庆:黑龙江八一农垦大学, 2009.
[19] 李祥晓 . 黑龙江省稻瘟病菌无毒基因分析及抗稻瘟病种质资源筛选. 北京:中国农业科学院, 2012.
[20] 张科, 刘丽, 韩俊楠 , 等. 黑龙江省稻瘟病菌无毒基因的组成及其频率初析. 种子世界, 2016(12):19-21.
[21] 王世维, 郑文静, 赵家铭 , 等. 辽宁省稻瘟病菌无毒基因型鉴定及分析. 中国农业科学, 2014,47(3):462-472.
doi: 10.3864/j.issn.0578-1752.2014.03.006
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