Crops ›› 2020, Vol. 36 ›› Issue (6): 163-169.doi: 10.16035/j.issn.1001-7283.2020.06.024

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Effects of Streptomyces sp. FXP04 on Seed Germination and Seedling Growth of Rice

Fu Xuepeng1,2(), Shen Tongfei1, Sun Xiaobo3, Liu Xiaohan1, Yang Xiaojie1,2()   

  1. 1Department of Life Sciences and Agroforestry, Qiqihar University, Qiqihar 161006, Heilongjiang, China
    2Heilongjiang Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar 161006, Heilongjiang, China
    3Agricultural Technology Extension Center of Tailai County, Tailai 162400, Heilongjiang, China
  • Received:2020-05-02 Revised:2020-10-14 Online:2020-12-15 Published:2020-12-09
  • Contact: Yang Xiaojie E-mail:02383@qqhru.edu.cn;yangxiaojie1980@163.com

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Abstract:

In order to determine the growth promoting effects of Streptomyces sp. FXP0404 on rice growth, the Streptomyces sp. FXP04 isolated in our previous experiments was selected as the test strain to study the effects of fermented liquid and cell suspension of Streptomyces sp. FXP04 on the seed germination and seedling growth of rice, using the methods of soaking seeds with fermented liquid and spraying soil with cell suspension. The results showed that both the original fermented liquid and LB liquid (control) inhibited seeds germination in the culture dish. Compared to water treatment, the rice seed germination was significantly increased in the treatment of 1/50 times fermented liquid, however, there were no significant effect on seed germination in the 1/10 and 1/100 times fermented liquid treatments. In the pot experiment, both 1/50 and 1/100 times cell suspension of Streptomyces sp. FXP04 promoted the germination of rice seeds, increased the plant height, main root length, dry weight, total root length, fibrous root number, root surface area and total root volume of rice seedlings, however, there was no significant difference between 1/50 and 1/100 times of Streptomyces sp. FXP04 cell suspension. The results demonstrated that Streptomyces sp. FXP04 could promote the seed germination and seedling growth of rice, and could be used as a candidate plant growth-promoting rhizobacteria on rice for further research and application.

Key words: Streptomyces, Streptomyces sp. FXP04, Rice, Seed germination, Seedling growth

Table 1

Effect of fermentation liquid on rice seed germination percentage %"

处理Treatment 处理后天数Days after treating (d)
6 8 10
LB培养液原液Original LB liquid 0.00±0.00d 0.00±0.00c 0.00±0.00d
发酵液原液Original fermentation liquid 0.00±0.00d 0.00±0.00c 0.00±0.00d
1/10倍LB培养液1/10 times LB liquid 62.60±4.42c 83.34±5.21b 86.66±5.64c
1/10倍发酵液1/10 times fermentation liquid 74.00±6.32a 89.34±4.58b 93.34±8.54b
1/50倍LB培养液1/50 times LB liquid 68.00±5.20b 86.66±6.35b 88.46±6.52bc
1/50倍发酵液1/50 times fermentation liquid 78.66±4.33a 94.00±8.25a 96.60±8.25a
1/100倍LB培养液1/100 times LB liquid 69.34±5.24b 85.34±7.53b 89.44±7.97bc
1/100倍发酵液1/100 times fermentation liquid 72.66±5.27b 90.00±8.25ab 90.00±8.25c
H2O 72.00±6.33b 92.00±9.62a 93.34±8.54b

Fig.1

Effect of fermentation liquid on rice seeds germination"

Table 2

Effect of cell suspension on rice seed germination percentage in soil %"

处理Treatment 处理后天数
Days after treating (d)
7 18
1/50倍菌液1/50 times cell suspension 20.00±2.00ab 79.00±3.00a
1/50倍LB培养液1/50 times LB liquid 12.00±1.00b 51.33±8.02c
1/100倍菌液1/100 times cell suspension 26.67±3.51a 76.33±4.51a
1/100倍LB培养液1/100 times LB liquid 25.67±5.03a 66.00±2.65b

Table 3

Effects of cell suspension on rice seedlings growth in soil"

处理Treatment 株高
Plant height (cm)		 主根长
Main root length (cm)
1/50倍菌液
1/50 times cell suspension		 15.88±1.36a 13.88±1.26a
1/50倍LB培养液
1/50 times LB liquid		 12.50±0.64b 9.325±1.00b
1/100倍菌液
1/100 times cell suspension		 15.20±0.87a 12.50±1.66a
1/100倍LB培养液
1/100 times LB liquid		 13.20±1.30b 8.60±0.99b

Fig.2

Effect of cell suspension on rice seedling growth in soil"

Fig.3

Effects of cell suspension on rice root growth CP50 and CP100 indicate 1/50 and 1/100 times of cell suspension, respectively; LB50 and LB100 indicate 1/50 and 1/100 times of LB liquid, respectively; Different lowercase letters above column indicate significant difference between groups (P < 0.05), the same below. D indicates the root diameter (mm)"

Fig.4

Effects of cell suspension on dry weight and root-shoot ratio of rice seedlings"

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