Crops ›› 2020, Vol. 36 ›› Issue (3): 197-200.doi: 10.16035/j.issn.1001-7283.2020.03.030

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Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province

Meng Feng, Zhang Yaling, Jin Xuehui()   

  1. Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control/Heilongjiang Plant Resistance Research Center, Daqing 163319, Heilongjiang, China
  • Received:2019-10-12 Revised:2020-01-21 Online:2020-06-15 Published:2020-06-10
  • Contact: Xuehui Jin E-mail:Jxh2686@163.com

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Abstract:

In order to detect the presence of the avirulence genes ACE1, AVR-Pi9, AVR-Pia, AVR-Pii and AVR1-CO39 of Magnaporthe oryzae in Heilongjiang Province, the DNA of 208 strains of Magnaporthe oryzae in Heilongjiang Province was detected by PCR using 5 pairs avirulence gene primers. The results showed that the highest frequency of ACE1 was 87.98%; the frequency of AVR1-CO39 was 0; the frequency of avirulence gene AVR-Pi9 was 79.81%; the frequency of amplification of AVR-Pia was 16.35% and the amplification frequency of AVR-Pii was 0.48%. The composition of the avirulence gene population of Magnaporthe oryzae in Heilongjiang Province is complex and has obvious regional characteristics.

Key words: Heilongjiang Province, Magnaporthe oryzae, Avirulence gene, Detection

Table 1

The primers for amplification of Magnaporthe oryzea avirulence genes"

无毒基因
Avirulence
gene		 引物序列 (5′-3′)
Primer sequence (5′-3′)		 目的片段长度
Length of targeted fragment (bp)
ACE1 F: GTGGAATCTTGTGGTGCTACGG
R: ACGGGAACATCGACGGAAAG		 1 057
AVR-Pi9 F: CGGCAGGTGGGTTTATCATC
R: CCAAAGCAGACAGCGACTCTA		 1 070
AVR-Pia F: TAGCCATTTCGGTCTCAT
R: CGGGTAAACCCTGTCATA		 1 597
AVR-Pii F: TATGCAGGCCCAAATCCGTAG
R: CCCGCAATAGTCCATAACTGTCC		 526
AVR1-CO39 F: CCTTGCCGCTACTGAAAC
R: CCCTATCGCAGAGGAACA		 419

Table 2

The frequencies of avirulence genes in Magnaporthe oryzae in Heilongjiang Province in 2017"

无毒基因
Avirulent gene		 出现个数
Number of occurrences		 出现频率
Occurrence frequency (%)
ACE1 183 87.98
AVR-Pi9 166 79.81
AVR-Pia 34 16.35
AVR-Pii 1 0.48
AVR1-CO39 0 0.00

Table 3

The distribution of avirulence genes in different regions of Heilongjiang Province"

序号
No.		 地区Area 总数
Total		 ACE1 AVR-Pi9 AVR-Pia AVR-Pii AVR1-CO39
数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency		 数目
Number		 频率(%)
Frequency
S1 齐齐哈尔甘南 16 15 93.75 14 87.50 0 0.00 0 0.00 0 0
S2 齐齐哈尔哈拉海农场 6 5 83.33 5 83.33 4 66.67 0 0.00 0 0
S3 大庆杜蒙 19 18 94.74 13 68.42 0 0.00 0 0.00 0 0
S4 大庆肇源 11 11 100.00 11 100.00 0 0.00 0 0.00 0 0
S5 绥化望奎 7 7 100.00 7 100.00 4 57.14 0 0.00 0 0
S6 绥化庆安 14 13 92.86 13 92.86 8 57.14 0 0.00 0 0
S7 哈尔滨通河 16 12 75.00 11 68.75 3 18.75 0 0.00 0 0
S8 哈尔滨依兰 23 21 91.30 21 91.30 0 0.00 0 0.00 0 0
S9 哈尔滨方正 4 4 100.00 4 100.00 0 0.00 0 0.00 0 0
S10 哈尔滨尚志 10 10 100.00 10 100.00 1 10.00 0 0.00 0 0
S11 佳木斯桦川 10 9 90.00 5 50.00 0 0.00 0 0.00 0 0
S12 佳木斯汤原 22 15 68.18 16 72.73 8 36.36 0 0.00 0 0
S13 佳木斯桦南 15 13 86.67 13 86.67 0 0.00 0 0.00 0 0
S14 鹤岗绥滨 16 16 100.00 13 81.25 3 18.75 0 0.00 0 0
S15 鸡西虎林 9 5 55.56 5 55.56 3 33.33 0 0.00 0 0
S16 鸡西密山 10 9 90.00 5 50.00 0 0.00 1 10.00 0 0

Fig.1

The occurrence frequency of each avirulence gene in different areas of Heilongjiang Province"

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