Crops ›› 2024, Vol. 40 ›› Issue (2): 242-248.doi: 10.16035/j.issn.1001-7283.2024.02.030

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Screening of Antagonistic Bacteria against Potato Bacterial Wilt and Study on Its Control Effect in Greenhouse

Li Sheng(), Li Xiang, Zhu Meiru, Wang Xia, Li Haoyang, Tan Xinru, Wang Haiyan()   

  1. College of Life Sciences, Sichuan University/Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Chengdu 610065, Sichuan, China
  • Received:2023-03-06 Revised:2023-03-23 Online:2024-04-15 Published:2024-04-15

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Abstract:

Bacterial wilt of potato is a destructive soil-borne disease caused by Ralstonia solanacearum. Screening antagonistic bacteria against pathogen is a vital work for the biocontrol of potato bacterial wilt. Six isolates with good inhibitory effect on three R.solanacearum strains of potato, tomato and tobacco were isolated from the rhizosphere soil of healthy potato, tomato, eggplant and pepper plants. They were identified as Lysinibacillus sp., Pseudomonas sp. and Kosakonia sp. through molecular detection, and it had the ability to produce protease, siderophor and biofilm, respectively. In greenhouse, strain FC-17 significantly reduced disease incidence by 79.46% and 70.83% on potato cultivars “Longshu 7” and “Atlantic”, respectively, indicating good potential for biocontrol of potato bacterial wilt.

Key words: Potato bacterial wilt, Antagonistic bacteria, Antimicrobial metabolites, Control effect

Table 1

Experimental design of pot control effect"

处理Treatment 添加物（盆）Supplement (pot)
R 20 mL无菌水+10 mL青枯菌菌液
FR 20 mL拮抗菌菌液+10 mL青枯菌菌液
F 20 mL拮抗菌菌液
CK 30 mL无菌水

Fig.1

Inhibitory effect of antagonistic bacteria against three strains of R.solanacearum Agar medium (a)-(c) were embedded with R. solanacearum strains from potato, tomato and tobacco, respectively. The loading volume of antagonistic bacteria was 50 μL (OD600nm=5.0) per well."

Table 2

Inhibition zone diameter of six antagonistic bacterias against three strains of R.solanacearum mm"

菌株名称
Strain name		 青枯菌来源Source of R. solanacearum
马铃薯Potato 番茄Tomato 烟草Tobacco
XC-1 4.40±0.74c 3.91±0.30d 4.38±0.74d
XC-2 7.38±0.98b 9.41±0.45b 9.03±0.59b
FC-11 6.61±0.34b 5.54±0.82c 6.64±0.34c
FC-17 6.90±0.79b 8.26±0.62b 8.41±0.34b
HN-9 7.11±0.91b 8.83±0.80b 8.96±0.81b
NX-18 10.14±0.74a 11.98±0.80a 10.70±0.53a

Fig.2

Electrophoresis of PCR products amplified from antagonistic bacteria 16S rRNA and internal transcribed spacer (a): Amplification of 16S rRNA, M represents λ-Eco I T-14 digest marker; (b): Amplification of internal transcribed spacer between 16S and 23S rRNA, M represents 1 kb plus DNA Ladder; 1-6 represent XC-1, XC-2, FC-11, FC-17, HN-9 and NX-18, respectively."

Fig.3

Colony morphology of antagonistic bacteria Strains were incubated at 30 ℃ for 48 h after streaking."

Fig.4

Determination of antibacterial substances of antagonistic bacteria (a) protease; (b) siderophore; (c) biofilm.“+”: Bacillus pumilus SCU11,“-”: Escherichia coli MG1655, CK: blank control."

Fig.5

Effects of FC-17 on the suppression of potato bacterial wilt (a) and (b) were Longshu 7 and Atlantic, respectively, pictures were taken at 45 days after seeding, and the enlargement of infected leaf is shown on the right of the picture."

Table 3

Statistics for the suppression of FC-17 on potato bacterial wilt in pots"

马铃薯品种
Potato cultivar		 处理
Treatment		 病情指数
Disease index		 防治效果
Control effect (%)
陇薯7号
Longshu 7		 R 45.37±18.49 －
FR 9.52±16.49* 79.46
F － －
CK － －
大西洋
Atlantic		 R 52.68±16.04 －
FR 15.18±15.64* 70.83
F － －
CK － －
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