Crops ›› 2017, Vol. 33 ›› Issue (2): 157-162.doi: 10.16035/j.issn.1001-7283.2017.02.028

Previous Articles     Next Articles

Isolation and Identification of Antagonistic Bacteria of Fusarium Head Blight of Winter Wheat in Hebei Province

Zhang Xuejiao,Shi Jingjing,Chang Na,Zhang Yaqin,Qi Yongzhi,Zhen Wenchao,Yin Baozhong   

  1. College of Plant Protection, Agricultural University of Hebei,Baoding 071001,Hebei,China
  • Received:2016-11-30 Revised:2017-02-17 Online:2017-04-15 Published:2018-08-26
  • Contact: Baozhong Yin

RichHTML

3

PDF (PC)

215

Abstract:

115 soil samples were collected from 15 counties in Hebei province during the wheat growing season from 2015 to 2016. The soil samples were separated by dilution method, then 218 strains of bacteria were obtained. 27 strains with the average inhibition rate of 44.09% on Fusarium were obtained after the filteration of the 218 strains by plate confrontation method. Among them, XJ-16 and LY-2 had good antagonistic effect, the inhibition rates were 73.76% and 73.73%, respectively. The morphological, physiological, biochemical and molecular identification of strain XJ-16 and LY-2 were carried out. The results showed that both two strains were Bacillus amyloliquefaciens.

Key words: Hebei province, Wheat, Fusarium head blight, Antagonistic bacteria, Separate, Identification

Table 1

Antimicrobial rate of antagonistic microorganisms %"

菌株编号Strain code 抑菌率Antimicrobial rate 菌株编号Strain code 抑菌率Antimicrobial rate 菌株编号Strain code 抑菌率Antimicrobial rate
XJ-16 73.76±1.35a WD-15 49.83±2.48bc ZQ-23 33.53±3.30d
LY-2 73.73±1.45a XJ-18 49.03±1.48bc WD-17 32.73±2.79d
ZQ-14 63.07±1.58b XJ-17 47.43±2.32bc XJ-13 29.56±2.11d
XJ-3 61.23±2.77b WD-8 47.40±3.36bc QX-1 29.03±1.32d
XJ-7 59.70±2.81b QX-16 46.90±4.94bc WD-24 26.83±2.90d
LY-9 58.73±1.55b XJ-19 45.20±1.99bc LY-10 26.73±3.40d
XJ-20 55.67±2.82b LY-12 43.10±2.74c LY-6 25.93±2.85d
QX-6 52.33±3.04b ZQ-8 37.83±1.55d QX-5 20.97±4.05e
LY-11 50.43±2.15bc WD-20 35.93±2.73d ZQ-19 20.93±2.34e

Fig.1

The antibacterial effects of XJ-16 and LY-2"

Fig.2

Colony morphology of XJ-16 and LY-2"

Fig.3

Bacterial morphology of XJ-16 and LY-2"

Table 2

Physiological and biochemical characteristics of strains XJ-16 and LY-2"

指标Index XJ-16 LY-2 指标Index XJ-16 LY-2
运动性 - - 水解 明胶 + +
接触酶 + + 淀粉 + +
氧化酶 - - 酪素 + +
厌氧生长 + + 利用 柠檬酸盐 - -
甲基红试验反应 - - 丙二酸盐 - -
乙酰甲基甲醇试验 + + 酪氨酸水解 - -
产生吲哚 - - 苯丙氨酸脱氨酶 - -
发酵 D-葡萄糖 + + 硝酸盐还原 + +
L-阿拉伯糖 + + 水解纤维素 + +
D-木糖 + + 生长 pH5.7营养肉汤 + +
D-甘露醇 + + 葡萄糖产气 - -
KOH溶液简易鉴定 - - 革兰氏染色 + +

Fig.4

Phylogenetic tree of the strains of LY-2 and XJ-16 based on gyrB sequence"

[1] Kheiri A, Moosawijorf S A, Mallihipour A , et al. Application of chitosan and chitosan nanoparticles for the control of Fusarium head blight of wheat (Fusarium graminearum) in vitro and greenhouse. International Journal of Biological Macromolecules, 2016,93:1261-1272.
doi: 10.1016/j.ijbiomac.2016.09.072
[2] Palazzini J M, Alberione E, Torres A , et al. Biological control of Fusarium graminearum sensu stricto,causal agent of Fusarium head blight of wheat,using formulated antagonists under field conditions in Argentina. Biological Control, 2015,94:56-61.
[3] Darwish W S, Ikenaka Y, Nakayama S M , et al. An overview on mycotoxin contamination of foods in Africa. Journal of Veterinary Medical Science, 2014,76(6):789-797.
doi: 10.1292/jvms.13-0563 pmid: 4108760
[4] Pleadin J, Vahčić N, Perši N , et al. Fusarium,mycotoxins' occurrence in cereals harvested from Croatian fields. Food Control, 2013,32(1):49-54.
doi: 10.1016/j.foodcont.2012.12.002
[5] 袁淑杰, 梁平, 任红敏 , 等. 冀京津春季冬小麦赤霉病发生期气象条件分析. 安徽农业科学, 2010,38(3):1359-1361.
[6] 徐芳, 齐永志, 张书敏 , 等. 新耕作制度下河北省冬小麦病虫草害发生状况的研究. 中国农学通报, 2012,28(21):199-203.
doi: 10.3969/j.issn.1000-6850.2012.21.036
[7] 张洁, 伊艳杰, 王金水 , 等. 小麦赤霉病的防治技术研究进展. 中国植保导刊, 2014,34(1):24-28.
doi: 10.3969/j.issn.1672-6820.2014.01.005
[8] 赵春富, 王永阳, 刘瑞华 , 等. 紫茎泽兰提取液对两种植物病原真菌的抑制作用研究. 湖北农业科学, 2012,51(6):1133-1135.
[9] Yoshida S, Ohba A, Liang Y M , et al. Specificity of Pseudomonas isolates on healthy and Fusarium head blight-infected spikelets of wheat heads. Microbial Ecology, 2012,64(1):214-225.
doi: 10.1007/s00248-012-0009-y
[10] Wachowska U, Kucharska K, Jȩdryczka M , et al. Microorganisms as biological control agents against Fusarium pathogens in winter wheat. Polish Journal of Environmental Studies, 2013,22(2):591-597.
doi: 10.1007/978-1-4614-4717-7_4
[11] Schisler D A, Slininger P J, Boehm M J , et al. Co-culture of yeast antagonists of Fusarium head blight and their effect on disease development in wheat. Plant Pathology Journal, 2011,10(4):128-137.
doi: 10.3923/ppj.2011.128.137
[12] Schisler D A, Core A B, Boehm M J , et al. Population dynamics of the Fusarium head blight biocontrol agent Cryptococcus flavescens,OH 182.9 on wheat anthers and heads. Biological Control, 2014,70(1):17-27.
doi: 10.1016/j.biocontrol.2013.11.011
[13] Palazzini J M, Haas G D, Torres A M , et al. Biocontrol and population dynamics of Fusarium spp.on wheat stubble in Argentina. Plant Pathology, 2013,62(4):859-866.
doi: 10.1111/ppa.2013.62.issue-4
[14] 陈年春 . 农药生物测定技术.北京: 北京农业大学出版社, 1990.
[15] 东秀珠, 蔡妙英 . 常见细菌系统鉴定手册.北京: 科学出版社, 2001.
[16] Wang L T, Lee F L, Tai C J , et al. Comparison of gyrB gene sequences,16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. International Journal of Systematic & Evolutionary Microbiology, 2007,57(8):1846-1850.
[17] Sharga B M, Lyon G D . Bacillus subtilis BS 107 as an antagonist of potato blackleg and soft rot bacteria. Canadian Journal of Microbiology, 1998,44(8):777-783.
doi: 10.1139/w98-064
[18] 刘伟成, 潘洪玉, 席景会 , 等. 小麦赤霉病拮抗性芽孢杆菌生防作用的研究. 麦类作物学报, 2005,25(4):95-100.
doi: 10.7606/j.issn.1009-1041.2005.04.156
[19] 陈琼珍 . 一株小麦病原菌的鉴定及其生防菌剂的初步研究. 郑州:河南工业大学, 2011.
[20] 温辉芹, 裴自友, 张立生 , 等. 小麦内生细菌鉴定及其对小麦赤霉病菌的拮抗作用. 山西农业科学, 2012,40(7):765-767.
doi: 10.3969/j.issn.1002-2481.2012.07.18
[21] 张俊 . 两株生防链霉菌的分离、鉴定及其生防效果的初步研究. 郑州:河南农业大学, 2012.
doi: 10.7666/d.y2157290
[22] 胡晓丹 . 禾谷镰刀菌拮抗菌的筛选鉴定及拮抗特性研究. 南京:南京农业大学, 2014.
[23] Khan N I, Schisler D A, Boehm M J , et al. Selection and evaluation of microorganisms for biocontrol of Fusarium head blight of wheat incited by Gibberella zeae. Plant Disease, 2001,85(12):1253-1258.
doi: 10.1094/PDIS.2001.85.12.1253
[24] Zalilakolsi I, Ben M A, Ali H , et al. Antagonist effects of Bacillus spp.strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L.subsp.durum). Microbiological Research, 2016,192:148-158.
doi: 10.1016/j.micres.2016.06.012
[25] 张荣胜, 戴秀华, 陈志谊 . 解淀粉芽孢杆菌Lx-11对水稻细菌性条斑病的防治效果. 江苏农业科学, 2014,42(10):115-116.
doi: 10.3969/j.issn.1002-1302.2014.10.035
[26] 向亚萍, 周华飞, 刘永锋 , 等. 解淀粉芽孢杆菌B1619脂肽类抗生素的分离鉴定及其对番茄枯萎病菌的抑制作用. 中国农业科学, 2016,49(15):2935-2944.
[27] 马桂珍, 葛平华, 刘兆普 . 海洋解淀粉芽孢杆菌GM-1培养基及摇瓶发酵条件优化.作物杂志, 2011(3):36-41.
[1] Zhao Xin, Chen Shaofeng, Wang Hui, . Research on the Yield and Quality of Different Tartaty#br# Buckwheat Varieties in Northern Shanxi Area [J]. Crops, 2018, 34(5): 27-32.
[2] Wang Hanxia, Shan Fuhua, Tian Liping, Ma Qiaoyun, . Analysis of Stability and Adaptability of Winter#br# Wheat Varieties in the Regional Trials of#br# the Northern Wheat Region of China [J]. Crops, 2018, 34(5): 40-44.
[3] An Xia, Zhang Haijun, Jiang Fangshan, Lü Lianjie, Chen Jun. Effects of Different Sowing Dates and Sowing#br# Densities on the Population Structure and#br# Yield of Two Spike Type Winter Wheats [J]. Crops, 2018, 34(5): 132-136.
[4] Wang Jianan, Li Xiaoyan, Wei Shimei, Zhao Huijie, Zhao Mingqi, Wang Yuexia. Regulation of Exogenous 5-Aminolevulinic#br# Acid on Photosynthesis and D1 Protein of#br# Wheat Seedlings under Drought Stress [J]. Crops, 2018, 34(5): 121-126.
[5] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops [J]. Crops, 2018, 34(4): 13-19.
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation [J]. Crops, 2018, 34(4): 121-125.
[7] Huiqin Wen,Tianling Cheng,Ziyou Pei,Xue Li,Lisheng Zhang,Mei Zhu. Analysis of Comprehensive Characteristics of Wheat Varieties Registered in Shanxi Province in Recent Years [J]. Crops, 2018, 34(4): 32-36.
[8] Haiyan Liang, Hai Li, Fengxian Lin, Xiangyu Zhang, Zhi Zhang, Xiaoqiang Song. Field Identification of Different Broom Corn Millet Varieties Lodging Resistance and Evaluation Index Selection and Analysis [J]. Crops, 2018, 34(4): 37-41.
[9] Fei Yang,Wenli Ma,Yongwei Chen,Zhansheng Zhang,Hao Wang. The Effects of Uniform Sowing and Drip Irrigation on the Spike Differentiation and Yield of Spring Wheat [J]. Crops, 2018, 34(4): 84-88.
[10] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production [J]. Crops, 2018, 34(4): 1-7.
[11] Junchan Wang,Zhifu Gao,Dongsheng Li,Dongmei Zhu,Hongya Wu. The Application of Agricultural Information Technology in Wheat Breeding [J]. Crops, 2018, 34(3): 37-43.
[12] Bin Zhang,Jinxiu Li,Zhen Wang,Hao Feng,Jinbang Li. Correlation and Cluster Analysis of Agronomic Traits in Wheat Lines [J]. Crops, 2018, 34(3): 57-60.
[13] Zheng Wang,Zhaobo Chen,Shengquan Zhang,Liping Ren,Xinhuan Gao,Zhijie Ye,Fengting Zhang. Feasibility Analysis of BS Series Hybrid Wheat Seed Production in Weinan of Shaanxi Province [J]. Crops, 2018, 34(3): 174-179.
[14] Xiaohua Shi,Haiying Yang,Wenqin Kang,Yonglin Qin,Mingshou Fan,Liguo Jia. Effects of Nitrogen Fertilization on Crop Yields and Soil Nitrogen Balance in Potato-Wheat System [J]. Crops, 2018, 34(2): 108-116.
[15] Shaohui Huang,Yunma Yang,Ketong Liu,Yanming Sun,Junfang Yang,Liangliang Jia. Yield Potential, Yield Gap and Nitrogen Use Efficiency Gap of Winter Wheat in Hebei Province [J]. Crops, 2018, 34(2): 118-122.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .