Crops ›› 2025, Vol. 41 ›› Issue (5): 135-141.doi: 10.16035/j.issn.1001-7283.2025.05.018

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Expression Analysis of the CYP450 Family Gene Os78A5 in Rice

Zhi Xianhong1(), Ji Zixian1, Xu Zhenwang1, Tan En1, Liang Rishen1, Ma Shuaipeng2, Tang Huiwu1()   

  1. 1 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
    2 Guangzhou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Guangzhou 510145, Guangdong, China
  • Received:2024-06-14 Revised:2024-07-24 Online:2025-10-15 Published:2025-10-21

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Abstract:

Cytochrome P450 (CYP450) is widely present in various organisms and plays an essential role in plant secondary metabolism and stress response. A gene encoding the CYP450 protein, Os78A5 (LOC_ Os11g29720), was cloned from rice (Oryza sativa). The gene structure, evolution process and cis-regulatory elements of Os78A5 were analyzed by bioinformatics method, and the expression of Os78A5 in different tissues and response to different plant hormones and abiotic stresses were analyzed by quantitative real-time PCR (qRT-PCR). The results showed that coding region of Os78A5 was 1617 bp, encoding 538 amino acids. Os78A5 has closer evolutionary relationship with the homologous protein CYP78A5 of Setaria viridis and Sorghum bicolor. The promoter region of Os78A5 contained 48 hormone response elements, 23 environmental stress response elements and 35 water response cis-regulatory elements. The expression of Os78A5 in rice leaves was significantly higher than that in other tissues, and it was induced by 6-benzylaminopurine, gibberellic acid, indole acetic acid, abscisic acid, high temperature and low temperature, suggesting that Os78A5 might be involved in abiotic stress response in rice.

Key words: Rice, CYP450, Abiotic stress, Plant hormone, Expression analysis

Table 1

Primer sequences used for qRT-PCR"

引物名称Primer name 引物序列（5′-3′）Primer sequence (5′-3′)
Os78A5-F TGCTGTCCTCTGGGAGATGAT
Os78A5-R GAAGGTTGGGGATGTCCGAG
Actin1-F GCATCTCTCAGCACATTCCA
Actin1 -R ACCACAGGTAGCAATAGGTA

Fig.1

Os78A5 gene structure and protein domain The gray boxes indicate 3′ UTR region and 5′ UTR region, the black boxes indicate exon, the black thin lines indicate intron, the white boxes indicate TMH domain."

Fig.2

Evolutionary analysis of Os78A5 Percentage values are the reliability of evolutionary branches."

Table 2

The cis-acting elements of Os78A5 promoter"

元件名称
Name of element		 功能
Function		 数量Number
ABRELATERD1 脱水响应 2
ACGTATERD1 脱水响应 4
CBFHV 脱水响应 4
DRECRTCOREAT 脱水响应 2
MYB1AT 脱水响应 4
MYB2CONSENSUSAT 脱水响应 2
MYCCONSENSUSAT 脱水响应和冷胁迫响应 12
MYBCORE 水分响应 3
MYB2AT 水分响应 2
SEBFCONSSTPR10A IAA响应 2
SURECOREATSULTR11 IAA响应 6
ARFAT IAA响应 2
ASF1MOTIFCAMV IAA、水杨酸响应 5
WBOXATNPR1 水杨酸诱导响应 2
GAREAT GA响应元件 1
MYBGAHV GA响应 1
PYRIMIDINEBOXOSRAMY1A GA响应 1
WRKY71OS GA响应 22
CPBCSPOR 6-BA响应 1
LTRECOREATCOR15 低温响应、ABA响应 3
CCAATBOX1 高温胁迫响应 6
GT1GMSCAM4 盐胁迫响应 2

Fig.3

Gene expression pattern analysis of Os78A5"

Fig.4

Expression level analysis of Os78A5 under different hormone treatments “*”: P < 0.05,“**”: P < 0.01,“***”: P < 0.001. The same below."

Fig.5

Expression level analysis of Os78A5 under different stress treatments"

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