Crops ›› 2019, Vol. 35 ›› Issue (4): 139-147.doi: 10.16035/j.issn.1001-7283.2019.04.021

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Effects of Epi-Brassinolide Treatments on Floret Opening Time of Indica and Japonica Rice

Xia Yuanye,Du Zhimin,Yang Yuchen,Gong Yanlong,Yan Zhiqiang,Xu Hai   

  1. Institute of Rice Research, Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs/Key Laboratory of Northern Japonica Super Rice Breeding, Ministry of Education/Key Laboratory of Northern Japonica Genetics and Breeding of Liaoning Province, Shenyang 110866, Liaoning, China
  • Received:2019-01-14 Revised:2019-06-05 Online:2019-08-15 Published:2019-08-06
  • Contact: Hai Xu

Abstract:

In this study, both six indica varieties and six japonica varieties were used to investigate the dynamics of floret opening. Three concentrations of epi-BR, including 0.01mg/L, 0.10mg/L and 1.00mg/L, were sprayed on the whole plants at 17:00 previous day and 6:00 and 8:00 in the morning investigation day, respectively. Water, no epi-BR, was used as the control (CK). A total of six traits related to the floret opening time, including the flowering begin time (FBT), the vigorous flowering time (VFT), the flowering end time (FET), the time from flowering begin to vigorous flowering (BVT), the time from vigorous flowering to flowering end (VET), and the time from flowering begin to end (BET), were investigated after application of epi-BR on different treatments. Compared to the control, the epi-BR with different concentration could promote the FBT, VFT and EFT of indica and japonica varieties. The floret opening time was dramatically different among the epi-BR treatments with different concentration. The effect of 0.10mg/L epi-BR on promoting flowering time was the best treatment in indica varieties. While the effect of 1.00mg/L epi-BR on promoting flowering time was the best treatment in japonica varieties. 0.10mg/L and 1.00mg/L epi-BR could promote BVT in indica varieties, but only 1.00mg/L BR could promote BVT in ssp. japonica rice. Epi-BR could delay the VET and BET of rice compared to the no epi-BR treatment (CK). The effect of spraying at 6:00 of investigation day on promoting flowering time was the best in ssp. indica rice. The effect of spraying at 17:00 previous day of investigation day on promoting flowering time was the best in ssp. japonica rice. Response of floret opening of ssp. indica rice was more sensitive than that of ssp. japonica rice on epi-BR. Treatment with medium concentration (0.10mg/L) of epi-BR at 6:00 of investigation day was the best treatment to promote flowering of ssp. indica rice. The treatment with heavy concentration (1.00mg/L) of epi-BR at 17:00 previous day of investigation day was the best treatment to promote flowering of ssp. japonica rice.

Key words: Epi-brassinolide, Floret opening time, ssp. indica rice, ssp. japonica rice

Fig.1

Effects of epi-BR with different concentrations on floret opening time of ssp. indica and ssp. japonica rice"

Table 1

Different effects of epi-BR with different concentrations on floret opening time of ssp. indica and ssp. japonica rice min"

类型
Type
品种
Cultivar
Epi-BR浓度(mg/L)
Epi-BR concentration
始花时
FBT
盛花时
VFT
终花时
FET
始-盛时间
BVT
盛-终时间
VET
始-终时间
BET
籼稻 泸香618B CK 66.11aA 116.78aA 216.11aA 50.67aA 99.33cC 150.00aA
ssp. indica Luxiang 618B 0.01 50.67bB 100.78bB 201.56bB 50.11aA 100.78cC 150.89aA
0.10 42.44cC 88.56dD 193.56cC 46.11bB 105.00bB 151.11aA
1.00 49.11bB 90.11cC 200.56bB 41.00cC 110.44aA 151.44aA
窄叶青8号 CK 74.67aA 124.44aA 234.11aA 49.78aA 109.67dD 159.44cC
Zhaiyeqing 8 0.01 60.22bB 110.78bB 224.89bB 50.56aA 114.11cC 164.67aAB
0.10 45.11cC 91.33dD 210.22dD 46.22bB 118.89bB 165.11aA
1.00 59.44bB 100.56cC 222.89cC 41.11cC 122.33aA 163.44bB
黄华占 CK 120.89aA 165.89aA 246.22aA 45.00aA 80.33dD 125.33cC
Huanghuazhan 0.01 91.89cC 137.22bB 224.67cC 45.33aA 87.44cC 132.78bB
0.10 79.00dD 120.11dD 211.33dD 41.11bB 91.22bB 132.33bB
1.00 97.56bB 129.33cC 231.78bB 31.78cC 102.44aA 134.22aA
2998B CK 70.33aA 115.56aA 200.44aA 45.22aA 84.89dD 130.11cC
0.01 44.00cC 89.33bB 185.00cC 45.33aA 95.67cC 141.00aA
类型
Type
品种
Cultivar
Epi-BR浓度(mg/L)
Epi-BR concentration
始花时
FBT
盛花时
VFT
终花时
FET
始-盛时间
BVT
盛-终时间
VET
始-终时间
BET
0.10 39.22dD 76.33dD 179.22dD 37.11bB 102.89bB 140.00aAB
1.00 49.11bB 80.00cC 187.67bB 30.89cC 107.67aA 138.56bB
明恢100 CK 99.89aA 160.44aA 240.67aA 60.56aA 80.22dD 140.78cB
Minghui100 0.01 69.22cC 128.56bB 224.89cC 59.33bA 96.33cC 155.67aA
0.10 62.89dD 107.67dD 217.56dD 44.78cB 109.89bB 154.67abA
1.00 74.11bB 109.89cC 228.44bB 35.78dC 118.56aA 154.33bA
冈46B Gang 46B CK 130.22aA 174.89aA 263.11aA 44.67aA 88.22dD 132.89bB
0.01 105.00cC 149.78bB 249.89cB 44.78aA 100.11cC 144.89aA
0.10 90.33dD 126.44dD 236.22dC 36.11bB 109.78bB 145.89aA
1.00 106.67bB 136.67cC 252.00bB 30.00cC 115.33aA 145.33aA
粳稻 越光Koshihikari CK 140.44aA 192.11aA 281.33aA 51.67aA 89.22cC 140.89bB
ssp.japonica 0.01 125.11bB 176.44bB 275.22bB 51.33aA 98.78bB 150.11aA
0.10 119.33cC 171.11cC 269.67cC 51.78aA 98.56bB 150.33aA
1.00 113.44dD 159.56dD 263.67dD 46.11bB 104.11aA 150.22aA
里歌Lige CK 190.22aA 250.00aA 350.00aA 59.78aA 100.00cC 159.78bB
0.01 178.67bB 239.11bB 345.22bB 60.44aA 106.11bB 166.56aA
0.10 177.89bB 237.89bB 344.33bB 60.00aA 106.44bB 166.44aA
1.00 166.56cC 211.89cC 333.00cC 45.33bB 121.11aA 166.44aA
丰锦Toyonishike CK 175.44aA 230.67aA 299.11aA 55.22aA 68.44dC 123.67bB
0.01 148.56bB 204.11bB 284.22bB 55.56aA 80.11cB 135.67aA
0.10 147.11cC 202.11cC 283.67bB 55.00aA 81.56bB 136.56aA
1.00 141.67dD 187.78dD 277.56cC 46.11bB 89.78aA 135.89aA
秋光Akihikari CK 170.22aA 215.33aA 304.89aA 45.11aA 89.56cC 134.67cB
0.01 160.44bB 204.89bB 296.56bB 44.44aA 91.67bB 136.11abA
0.10 159.67bB 204.67bB 296.33bB 45.00aA 91.67bB 136.67aA
1.00 146.89cC 185.56cC 282.56cC 38.67bB 97.00aA 135.67bAB
秋田小町 CK 150.89aA 220.00aA 330.44aA 69.11aA 110.44cC 179.56bB
Akitakaomaqi 0.01 131.00bB 198.67cB 324.22cC 69.56aA 125.56bB 195.11aA
0.10 129.11cC 200.44bB 327.00bB 69.44aA 126.56bB 196.00aA
1.00 121.33dD 171.44dD 316.78dD 50.11bB 145.33aA 195.44aA
一目惚Yimuhu CK 180.56aA 239.89aA 321.11aA 59.33aA 81.22cC 140.56bB
0.01 159.33bB 219.89bB 309.56bB 60.56aA 89.67bB 150.22aA
0.10 159.22bB 219.56bB 309.78bB 60.33aA 90.22bB 150.56aA
1.00 150.22cC 195.89dD 300.33cC 45.67bB 104.44aA 150.11aA

Fig.2

Effects of epi-BR treated at different time on floret opening time of ssp. indica and ssp. japonica rice"

Table 2

Different effects of epi-BR treated at different time on floret opening time of ssp. indica and ssp. japonica rice min"

类型
Type
品种
Cultivar
喷施时间
Spraying time
始花时
FBT
盛花时
VFT
终花时
FET
始-盛时间
BVT
盛-终时间
VET
始-终时间
BET
籼稻 泸香618B Luxiang 618B 调查前日17:00 51.00aA 101.83aA 205.17aA 46.92aA 103.33aA 150.25aA
ssp. indica 调查当日6:00 41.00bB 94.17bB 198.08bB 46.92aA 103.92aA 150.83aA
调查当日8:00 50.22aA 101.17aA 205.58aA 47.08aA 104.42aA 151.50aA
窄叶青8号Zhaiyeqing 8 调查前日17:00 61.83aA 109.00aA 225.25aA 47.17aA 116.25aA 163.42aA
调查当日6:00 55.25bB 102.42bB 218.33bB 47.17aA 115.92aA 163.08aA
调查当日8:00 62.50aA 108.92aA 225.50aA 46.42aA 116.58aA 163.00aA
黄华占Huanghuazhan 调查前日17:00 99.00aA 139.50aA 229.67aA 40.50aA 90.17aA 130.67aA
调查当日6:00 94.00bB 135.08bB 225.42bB 41.08aA 90.33aA 131.42aA
调查当日8:00 99.00aA 139.83aA 230.42aA 40.83aA 90.58aA 131.42aA
2998B 调查前日17:00 52.50aA 92.67aA 190.17aA 40.17aA 97.50aA 137.67aA
调查当日6:00 47.17bB 86.33bB 183.92bB 39.17aA 97.58aA 136.75aA
调查当日8:00 52.33aA 91.92aA 190.17aA 39.58aA 98.25aA 137.83aA
明恢100 Minghui 100 调查前日17:00 79.33aA 129.50aA 230.25aA 50.17aA 100.75aA 150.92aA
类型
Type
品种
Cultivar
喷施时间
Spraying time
始花时
FBT
盛花时
VFT
终花时
FET
始-盛时间
BVT
盛-终时间
VET
始-终时间
BET
调查当日6:00 71.75bB 121.00bB 223.42bB 49.25aA 102.42aA 151.67aA
调查当日8:00 78.50aA 129.42aA 230.00aA 50.92aA 100.58aA 151.50aA
冈46B Gang 46B 调查前日17:00 109.83aA 149.08aA 252.17aA 39.25aA 103.08aA 142.33aA
调查当日6:00 103.67bB 142.17bB 246.08bB 38.50aA 103.92aA 142.42aA
调查当日8:00 110.67aA 149.58aA 252.67aA 38.92aA 103.08aA 142.00aA
粳稻 越光Koshihikari 调查前日17:00 120.67cC 170.92cB 268.17bB 50.25aA 97.25aA 147.50aA
ssp. japonica 调查当日6:00 125.50bB 176.08bA 273.92aA 50.58aA 97.83aA 148.42aA
调查当日8:00 127.58aA 177.42aA 275.33aA 49.83aA 97.92aA 147.75aA
里歌Lige 调查前日17:00 175.00bB 231.42bB 339.58bC 56.42aA 108.17aA 164.58aA
调查当日6:00 154.75aA 235.92aA 344.25bA 56.25aA 108.33aA 164.58aA
调查当日8:00 180.33aA 236.83aA 345.58aA 56.50aA 108.75aA 165.25aA
丰锦Toyonishike 调查前日17:00 149.00cC 201.83cB 281.83cB 52.83aA 80.00aA 132.83aA
调查当日6:00 154.75bB 207.92bA 287.83bA 53.17aA 79.92aA 133.08aA
调查当日8:00 155.83aA 208.75aA 288.75aA 52.92aA 80.00aA 132.92aA
秋光Akihikari 调查前日17:00 156.00bB 199.25cB 291.42bB 43.25aA 92.17aA 135.42aA
调查当日6:00 160.58aA 203.75bA 296.92aA 43.17aA 93.17aA 136.33aA
调查当日8:00 161.33aA 204.83aA 296.92aA 43.50aA 92.08aA 135.58aA
秋田小町Akitakaomaqi 调查前日17:00 129.08bB 193.50bB 320.83bB 64.42aA 127.33aA 191.75aA
调查当日6:00 135.08aA 199.75aA 326.16aA 64.67aA 126.42aA 191.08aA
调查当日8:00 135.08aA 199.67aA 326.83aA 64.58aA 127.17aA 191.75aA
一目惚Yimuhu 调查前日17:00 157.67bB 214.75bB 305.67bB 57.08aA 90.92aA 148.00aA
调查当日6:00 164.33aA 220.33aA 312.08aA 56.00aA 91.75aA 147.75aA
调查当日8:00 165.00aA 221.33aA 312.83aA 56.33aA 91.50aA 147.83aA

Table 3

Optimal combination for flowering opening time control by epi-BR treatment of ssp. indica and ssp. japonica rice"

品种Cultivar 始花时最优组合Optimal combination of FBT 盛花时最优组合Optimal combination of VFT
泸香618B Luxiang 618B 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
窄叶青8号Zhaiyeqing 8 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
黄华占Huanghuazhan 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
2998B 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
明恢100 Minghui 100 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
冈46B Gang 46B 调查当日6:00喷施0.10mg/L的epi-BR 调查当日6:00时喷施0.10mg/L的epi-BR
越光Koshihikari 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR
里歌Lige 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR
丰锦Toyonishiki 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR
秋光Akihikari 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR
秋田小町Akitakaomaqi 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR
一目惚Yimuhu 调查前日17:00喷施1.00mg/L的epi-BR 调查前日17:00时喷施1.00mg/L的epi-BR

Table 4

Differences of sensitivity between ssp. indica and ssp. japonica rice populations in response to epi-BR min"

Epi-BR浓度(mg/L)
Epi-BR concentration
类型
Type
始花时差
DFBT
盛花时差
DVFT
终花时差
DFET
始-盛时间差
DBVT
盛-终时间差
DVET
始-终时间差
DBET
CK 籼稻ssp. indica -93.69 -143.00 -233.44 49.31 90.44 139.76
粳稻ssp. japonica -167.96** -224.67** -314.48** -56.70 89.81 146.52
0.01 籼稻ssp. indica -23.52 -23.59 -14.96** -0.07 8.63 8.56
粳稻ssp. japonica -17.44 -14.48 -8.65 -0.28 8.83 9.11
0.10 籼稻ssp. indica -33.85** -41.26** -25.33** -7.41** 15.83 8.43
粳稻ssp.japonica -19.24 -18.70 -9.35 -0.22 9.35 9.57
1.00 籼稻ssp. indica -21.02 -35.24 -12.89 -14.22 22.35 8.13
粳稻ssp. japonica -27.94** -39.31 -18.83** -11.37 20.48 9.11
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