Crops ›› 2021, Vol. 37 ›› Issue (3): 57-64.doi: 10.16035/j.issn.1001-7283.2021.03.009

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Effects of Tillage Methods on Soil Microbial Community Diversity in Oat Fields

Wang Lifang(), Zhang Dejian(), Zhang Tingting   

  1. School of Life Sciences, Inner Mongolia University/Key Laboratory of Herbage and Endemic Crop Biotechnology, Ministry of Education, Hohhot 010070, Inner Mongolia, China
  • Received:2020-07-08 Revised:2020-12-31 Online:2021-06-15 Published:2021-06-22
  • Contact: Zhang Dejian E-mail:1393238495@qq.com;zhangdejian00@163.com

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Abstract:

In order to explore the effects of different tillage methods on soil microbial community diversity in oat fields, to enrich the species and quantity of soil microorganisms, mechanical no-tillage and traditional tillage were used to take soil samples of 0-20cm at the jointing stage and filling stage of oat, respectively. 16S rRNA sequencing techniques and ITS sequencing techniques were used to sequence soil bacteria and fungi, respectively, and then analyzed the diversity and difference of microbial community. The results showed that the variety and abundance of soil microorganisms were higher in no-tillage treatment compared with traditional tillage treatment. Dominance abundance analysis had shown that the dominant fungi were Ascomycota and Basidiomycota, and the dominant bacteria were Actinomycetes, Proteobacteria, and Acidobacteria. Therefore, compared with traditional tillage, no-tillage treatment could improve soil microbial diversity and provide a theoretical basis for the selection of oat field tillage method.

Key words: Tillage methods, Oat field, Soil microorganisms, Diversity

Table 1

Soil fungal sequences number"

处理
Treatment		 有效序列
Valid
sequence		 优质序列
Quality
sequence		 比例
Percentage
(%)
翻耕拔节期
Plowing jointing period (FGB)		 18 641 14 640 78.54
免耕拔节期
No tillage jointing period (MGB)		 32 344 28 098 86.87
翻耕灌浆期
Plowing grouting period (YGF)		 15 904 12 098 76.07
免耕灌浆期
No tillage grouting period (YGM)		 15 995 11 996 75.00

Table 2

Number of bacterial sequences in soil samples during oat grouting period"

处理
Treatment		 有效序列
Valid sequence		 优质序列
Quality sequence		 比例
Percentage (%)
YGF 174 498 124 558 71.38
YGM 174 593 133 746 76.60

Fig.1

OTU clustering diagram of soil fungi (left) and bacterium (right) in oat field under different tillage methods"

Fig.2

Distribution curve of soil fungi and bacteria abundance in oat fields under different tillage methods"

Table 3

Soil microbial diversity indexes of oat under different tillage methods and different growth stages"

类别
Sort		 处理
Treatment		 丰富度指数
Abundant index		 多样性指数
Diversity index
ace chao simpson shannon
真菌 FGB 1343.8190 1022.2000 0.0542 3.9244
Fungus MGB 814.1254 826.0374 0.0254 4.6401
YGF 865.4583 680.9421 0.0671 3.5796
YGM 767.2573 790.4694 0.1053 3.4812
细菌 FGB 5811.2160 5859.6097 0.0032 6.9241
Bacteria MGB 5913.9673 5961.5290 0.0030 6.9325
YGF 5870.0840 5927.9570 0.0037 6.8661
YGM 5980.8010 6057.8190 0.0034 6.8962

Fig.3

Effects of different tillage methods on the distribution of oat soil fungi and bacterium"

Table 4

Effects of different tillage methods on the fungi and bacteria abundance of oat soil %"

类别Sort 平均值Mean YGM YGF MGB FGB
真菌Fungus 0.0 0.0 0.0 0.0 0.0
0.3 0.0 0.6 0.6 0.0
64.3 59.5 58.2 56.0 83.6
2.4 1.2 1.2 5.7 1.7
1.0 0.0 0.1 3.9 0.0
0.1 0.0 0.1 0.1 0.0
0.0 0.0 0.0 0.0 0.0
1.2 0.0 0.0 4.7 0.0
30.7 39.2 39.8 28.9 14.7
细菌Bacteria 0.0 0.0 0.0 0.0 0.0
12.3 14.4 11.2 10.8 12.9
37.3 33.9 38.6 39.2 37.3
0.3 0.3 0.3 0.5 0.2
0.0 0.0 0.0 0.0 0.0
0.0 0.0 0.0 0.0 0.0
3.8 4.3 3.5 3.1 4.3
0.0 0.0 0.0 0.0 0.0
0.0 0.0 0.0 0.1 0.0
0.0 0.0 0.0 0.0 0.0
0.1 0.1 0.1 0.3 0.1
0.1 0.2 0.1 0.0 0.1
0.0 0.0 0.0 0.0 0.0
0.1 0.1 0.1 0.1 0.1
6.3 6.8 5.6 6.9 5.8
0.1 0.1 0.1 0.0 0.1
0.0 0.0 0.0 0.0 0.0
0.0 0.0 0.0 0.0 0.0
0.5 0.6 0.5 0.8 0.3
4.2 4.6 4.5 3.4 4.2
0.0 0.1 0.1 0.0 0.0
0.0 0.0 0.0 0.0 0.0
0.6 0.8 0.6 0.3 0.6
5.7 5.4 6.4 5.8 5.0
23.5 24.2 23.2 23.2 23.4
0.0 0.0 0.0 0.0 0.0
0.0 0.0 0.0 0.0 0.0
0.0 0.0 0.0 0.0 0.0
4.9 4.0 5.2 5.3 5.1
0.0 0.0 0.0 0.0 0.0

Fig.4

PCA of fungi and bacterium"

Fig.5

Cluster analysis of fungi and bacterium under different treatments"

Fig.6

Community structure of fungi (left) and bacterium (right) in oat fields under different treatments"

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