Crops ›› 2020, Vol. 36 ›› Issue (4): 79-83.doi: 10.16035/j.issn.1001-7283.2020.04.011

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Construction of SSR Fingerprints and Diversity Analysis of a Cowpea Applied Core Collection

Gong Dan1,2(), Wang Suhua1, Cheng Xuzhen1(), Wang Lixia1()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2College of Agriculture, Yangtze University, Jingzhou 434020, Hubei, China
  • Received:2019-12-02 Revised:2020-06-05 Online:2020-08-15 Published:2020-08-11
  • Contact: Cheng Xuzhen,Wang Lixia E-mail:gongdan1992@sina.com;wanglixia03@caas.cn

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Abstract:

Assessment of the diversity and genetic background of different germplasm resources can provide useful information for further collection, preservation and utilization. We evaluated 88 cowpea core germplasm resources from different sources using 14 SSR markers. The total of 39 alleles were detected and two to five alleles per locus were detected for each pair of primers with an average of 2.79. The polymorphic information content (PIC) varied from 0.25 to 0.72, and averaged at 0.58. The UPGMA (unweight pair group method with arithmetic mean) result showed that all the reserved collections could be distinguished by 39 alleles except 3 couples of germplasms. The 88 collections were divided into four groups at the genetic similarity of 0.63, and the collections from the same origins tended to be clustered together.

Key words: Cowpea, Germplasm resources, SSR, Genetic diversity, Clustering analysis

Table 1

Information of 88 cowpea core germplasm resources"

序号
Number		 统一编号
Uniform number		 品种
Variety		 原产地
Origin		 序号
Number		 统一编号
Uniform number		 品种
Variety		 原产地
Origin
BJ-1 I00003 白黑眼 中国北京 JL-3 - JD6 中国吉林
BJ-2 I00030 红豇豆 中国北京 JL-4 - 黑豇豆9801 中国吉林
BJ-3 I01338 中豇1号 中国北京 HL-1 I00344 花豇豆 中国黑龙江
BJ-4 I01343 豇豆 中国北京 HL-2 I02828 豇豆 中国黑龙江
BJ-5 I01921 豇豆 中国北京 TJ-1 I00502 豇豆 中国天津
BJ-6 I01922 豇豆 中国北京 TJ-2 I00503 豇豆 中国天津
BJ-7 I01925 豇豆 中国北京 SH I00511 豇豆 中国上海
BJ-8 I01927 豇豆 中国北京 AH-1 I00764 白豇豆 中国安徽
BJ-9 I02620 豇豆 中国北京 AH-2 I00769 白豇豆 中国安徽
BJ-10 I02631 夏宝 中国北京 AH-3 I00774 白豇豆 中国安徽
BJ-11 I02632 春燕 中国北京 HB-1 I00970 黑饭豆 中国湖北
BJ-12 I03099 豇豆 中国北京 HB-2 I02740 坡豇豆 中国湖北
BJ-13 I03112 豇豆 中国北京 GX-1 I01117 红饭豆 中国广西
BJ-14 I03113 豇豆 中国北京 GX-2 I01187 狗面豆 中国广西
BJ-15 I03114 豇豆 中国北京 SN-1 I01230 打豇豆 中国陕西
BJ-16 I03118 豇豆 中国北京 SN-2 - JD4 中国陕西
BJ-17 I03120 豇豆 中国北京 SN-3 - JD5 中国陕西
BJ-18 I03126 豇豆 中国北京 IITA-1 I01273 IT82D-703 IITA
BJ-19 I03132 豇豆 中国北京 IITA-2 I01274 IT82D-716 IITA
BJ-20 I03133 豇豆 中国北京 IITA-3 I01278 IT82D-952 IITA
BJ-21 I03137 豇豆 中国北京 IITA-4 I01281 TVX3236 IITA
BJ-22 I03138 豇豆 中国北京 IITA-5 I01293 中豇2号(IT82D-789) IITA
BJ-23 I03139 豇豆 中国北京 IITA-6 I01298 IT84E-124 IITA
BJ-24 I03140 豇豆 中国北京 IITA-7 I01299 IT82D-453-2 IITA
BJ-25 I03141 豇豆 中国北京 IITA-8 I01302 IT83S-852 IITA
BJ-26 I03171 豇豆 中国北京 IITA-9 I01321 IT82D-889 IITA
BJ-27 I03181 豇豆 中国北京 HA-1 I01398 白豇豆 中国河南
BJ-28 I03182 豇豆 中国北京 HA-2 I01407 白豇豆 中国河南
BJ-29 I03282 豇豆 中国北京 HA-3 I02818 豫豇1号 中国河南
HE-1 I00058 豇豆 中国河北 NX-1 I01831 豇豆 中国宁夏
HE-2 I00081 串蔓花豇豆 中国河北 NX-2 I01836 豇豆 中国宁夏
HE-3 I00563 花豇豆 中国河北 SD I02372 胡豇豆 中国山东
HE-4 - JD1 中国河北 HN-1 I02496 豇豆 中国湖南
SX-1 I00093 爬豇豆 中国山西 HN-2 I02497 豇豆 中国湖南
SX-2 - JD3 中国山西 HN-3 I02504 枫树饭豆 中国湖南
IM-1 I00199 红豇豆 中国内蒙古 HN-4 I02510 豇豆 中国湖南
IM-2 I00200 豇豆 中国内蒙古 HN-5 I03101 早生王 中国湖南
IM-3 I02130 长豇豆 中国内蒙古 HN-6 I03102 耐热王 中国湖南
IM-4 - JD9 中国内蒙古 GD-1 I03144 长豆角 中国广东
LN-1 I00258 豇豆 中国辽宁 GD-2 I03145 早豆角 中国广东
LN-2 I00272 花豇豆 中国辽宁 JS-2 - 苏豇2号 中国江苏
LN-3 I00277 花豇豆 中国辽宁 JS-2 - 早豇1号 中国江苏
JL-1 I00321 花豇豆 中国吉林 JS-3 - 海门短肉豇 中国江苏
JL-2 I00336 大花豇豆 中国吉林 Unknow I01314 - 不明

Table 2

Polymorphic information of 88 cowpea core germplasm based on 14 SSR loci"

引物编号
Primer
number		 等位基因数
Number
of alleles		 有效等位基因数
Number of
effective alleles		 多态性信息含量
Polymorphism
information content
VM30 3 1.34 0.25
VM36 3 1.95 0.49
VM31 4 2.95 0.66
VM35 2 3.40 0.71
VM34 2 1.73 0.42
VM39 3 2.91 0.66
VM28 2 2.97 0.66
PVBR185 3 2.98 0.66
BMd-2 2 2.25 0.56
BMd-17 2 2.29 0.56
AG1 5 2.08 0.52
PVBR4 2 3.62 0.72
PVBR12 2 3.25 0.69
12M1 4 2.01 0.50
平均
Average		 2.79 2.55 0.58

Fig.1

Cluster tree of 88 cowpea core germplasm"

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