Crops ›› 2021, Vol. 37 ›› Issue (5): 205-210.doi: 10.16035/j.issn.1001-7283.2021.05.031

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Evaluation of Drought Tolerance of Three Elymus nutans in Tibet

Zhou Jing1(), Sun Kan1, Zhou Zhongyi2, Wang Chuanqi3, Miao Yanjun3()   

  1. 1Shenzhen Beilinyuan Landscape and Architectural Design Institute Co., Ltd., Shenzhen 518038,Guangdong, China
    2The Grassland Workstation of Yakeshi, Yakeshi 022150, Inner Mongolia, China
    3Tibet Agriculture and Animal Husbandry College, Nyingchi 860000, Tibet, China
  • Received:2021-05-07 Revised:2021-06-17 Online:2021-10-15 Published:2021-10-14
  • Contact: Miao Yanjun E-mail:34941321@qq.com;myj666@126.com

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Abstract:

This paper aimed to provide basis for drought resistance identification and breeding of native plants in Tibet, by exploring the physiological response to drought stress of wild Elymus nutans at seed germination stage and seedling stage. Two wild E. nutans seeds came from Baingoin county and Sog county in Naqu city of Tibet were used as experimental materials No. Ⅰ and No. Ⅱ, and the domesticated variety E.nutans G. cv. Baqing was used as reference material. The solution of polyethylene glycol (PEG-6000) and natural drought after potted plants to simulate drought stress environment at germination and seedling stages and determined the physiological indexes. The results were as followos: PEG-6000 inhibited the germination of No. I and No. II seeds, but the low concentration of PEG-6000 (-0.10MPa) was beneficial to the germination of E.nutans. PEG-6000 inhibited the growth of roots and buds of E. nutans, especially the buds. Under drought stress, the relative electrical conductivity and malondialdehyde content of E. nutans seedlings increased continuously, while the activities of superoxide dismutase, catalase and peroxide increased at first and then decreased. Through the analysis of average value of subordinate function. The three E.nutans tested materials had strong physiological drought tolerance at seed germination and seedling stages, and all of them belonged to grade II resistance. The order of drought tolerance was as follows: E.nutans G. cv. Baqing > E.nutans No. I > E.nutans No. II.

Key words: Elymus nutans Griseb., Alpine grassland, Germination and seedling stages, Antioxidant enzyme, Cell membrane damage

Table 1

Materials and source status"

材料
Material		 采集地
Source site		 地理坐标
Geographical coordinates		 海拔
Altitude
(m)		 生境
Habitat
Ⅰ号No.Ⅰ 班戈县
城北20km		 31°28′ N;
89°50′ E		 4 742 公路边
Ⅱ号No.Ⅱ 索县 32°02′ N;
94°03′ E		 3 985 高寒
草原
巴青垂穗披碱草
E.nutans G. cv. Baqing		 - - - -

Table 2

Effects of drought stress on seed germination of E. nutans"

材料Material 指标
Index		 环境水势Environmental water potential
0.00MPa -0.10MPa -0.20MPa -0.40MPa -0.80MPa -1.00MPa
Ⅰ号No.Ⅰ GP (%) 84.50±2.11aA 76.50±4.32aA 63.00±4.90abA 48.50±4.82bA 36.75±3.90bcA 25.50±0.87cB
GI 40.03±1.27aA 32.45±3.50aA 26.67±3.69abA 20.21±3.71bA 12.85±4.78bcA 10.53±1.22cA
VI 212.96±5.33aA 161.28±7.12abA 129.08±6.90bA 83.27±2.50cB 45.75±1.27dB 32.12±3.23dB
Ⅱ号No.Ⅱ GP (%) 85.25±2.21aA 76.50±2.64abA 66.25±2.33abA 53.75±3.40bA 38.50±4.45bcA 29.25±2.21cB
GI 43.44±3.34aA 35.67±4.07aA 30.89±2.61abA 23.79±4.26bA 17.67±2.89bcA 14.45±3.34cA
VI 253.26±7.12aA 217.39±3.14abA 145.18±3.83bA 92.31±1.67cB 60.61±1.48dB 47.97±7.12dB
巴青垂穗披碱草 GP (%) 92.50±2.53aA 93.50±4.13aA 72.50±3.45aA 66.25±3.36abA 46.00±4.79bA 32.50±1.49bB
E.nutans G. cv. Baqing GI 47.25±2.78abA 53.10±2.76aA 39.82±1.90abA 33.45±2.96bA 22.98±5.39bA 19.50±1.33bA
VI 287.94±4.32aA 326.57±5.76aA 226.58±7.84abA 173.84±4.62bA 108.70±3.59cB 53.43±4.20dB

Table 3

Effects of drought stress on growing of E. nutans seedlings"

材料Material 指标
Index		 环境水势Environmental water potential
0.00MPa -0.10MPa -0.20MPa -0.40MPa -0.80MPa -1.00MPa
Ⅰ号No.Ⅰ 根长 (cm) 5.32±1.15a 4.97±1.22a 4.84±1.31ab 4.12±0.94ab 3.56±1.36ab 3.05±0.95b
芽长 (cm) 12.92±2.26a 11.45±2.71ab 10.39±1.96ab 7.93±2.05b 5.72±1.59bc 4.01±2.30c
根芽比 0.41±0.01b 0.43±0.01b 0.47±0.03ab 0.52±0.02ab 0.62±0.01ab 0.76±0.04a
Ⅱ号No.Ⅱ 根长 (cm) 4.83±1.05a 4.76±1.24a 4.52±1.35a 3.88±1.69ab 3.43±1.48ab 2.74±0.97b
芽长 (cm) 12.34±1.47a 10.71±1.77ab 9.77±1.82ab 7.64±2.13b 6.07±1.91b 3.65±1.22c
根芽比 0.39±0.01b 0.44±0.02b 0.46±0.03ab 0.51±0.02ab 0.56±0.02ab 0.75±0.03a
巴青垂穗披碱草 根长 (cm) 5.69±1.72a 6.15±2.03a 6.03±2.46a 5.63±1.92a 5.10±2.33a 4.32±2.15a
E.nutans G. cv. Baqing 芽长 (cm) 13.80±2.35a 12.86±1.34a 11.69±3.01a 9.50±2.17ab 7.99±1.42b 5.32±1.09b
根芽比 0.41±0.02b 0.47±0.04b 0.52±0.02ab 0.59±0.01ab 0.64±0.01ab 0.81±0.02a

Table 4

Subordinate function value and subordinate degree of E. nutans under drought stress at germination stage"

材料
Material		 测定指标Determination index 隶属度
Subordinate
degree
GP GI VI 根长
Root
length		 芽长
Bud
length
Ⅰ号No.Ⅰ 0.526 0.500 0.482 0.532 0.570 0.522
Ⅱ号No.Ⅱ 0.548 0.490 0.484 0.500 0.454 0.495
巴青垂穗披碱草
E.nutans G.
cv. Baqing		 0.582 0.544 0.532 0.534 0.534 0.554

Table 5

Effects of drought stress on cell membrane damage degree of seedlings"

材料
Material		 指标
Index		 干旱胁迫天数Drought stress days
0d 7d 14d 21d
Ⅰ号No.Ⅰ REC (μS/cm) 9.67±2.41bB 34.83±5.69aA 42.01±6.37aA 50.22±5.48aA
MDA (nmol/g) 3.49±1.16cB 20.95±3.24bAB 35.33±4.31aA 36.32±4.65aA
Ⅱ号No.Ⅱ REC (μS/cm) 13.00±2.40cB 31.00±3.35bAB 49.81±5.79aA 62.70±6.39aA
MDA (nmol/g) 5.57±1.06bB 30.69±3.84aA 39.28±4.13aA 43.30±5.39aA
巴青垂穗披碱草 REC (μS/cm) 6.40±2.73cB 23.67±4.38bAB 38.28±5.09aA 47.79±5.24aA
E.nutans G. cv. Baqing MDA (nmol/g) 2.46±0.71cB 18.61±2.14bAB 23.67±3.69abA 27.96±2.95aA

Table 6

Changes of antioxidant enzyme activities in seedling leaves under drought stress"

材料
Material		 指标
Index		 干旱胁迫天数Drought stress days
0d 7d 14d 21d
Ⅰ号No.Ⅰ SOD [U/(g FW·h)] 27.94±3.21cB 45.15±2.97bAB 79.36±5.04aA 69.10±5.33aA
POD [U/(g FW·min)] 115.40±6.39bA 186.90±5.72aA 273.40±9.31aA 253.70±8.04aA
CAT [mg H2O2/(g FW·min)] 10.41±2.83bA 22.04±4.11aA 29.49±3.76aA 27.67±3.25aA
Ⅱ号No.Ⅱ SOD [U/(g FW·h)] 18.59±4.52cB 32.74±5.26bAB 60.43±4.92aA 57.06±5.67aA
POD [U/(g FW·min)] 117.20±6.33bA 189.80±7.39aA 256.90±9.34aA 231.70±8.56aA
CAT [mg H2O2/(g FW·min)] 6.56±2.47bA 17.09±4.41aA 23.50±3.97aA 18.71±4.08aA
巴青垂穗披碱草 SOD [U/(g FW·h)] 23.72±5.36cB 48.45±4.73bAB 87.12±5.69aA 82.33±4.92aA
E.nutans G. cv. Baqing POD [U/(g FW·min)] 186.10±7.04bA 279.90±9.31aA 369.80±10.22aA 353.60±9.87aA
CAT [mg H2O2/(g FW·min)] 9.81±2.57bA 26.67±3.45aA 34.51±4.73aA 33.21±4.09aA

Table 7

Subordinate function value and subordinate degree of E. nutans at seedling stage"

材料
Material		 指标Index 隶属度
Subordinate
degree
REC MDA SOD POD CAT
Ⅰ号No.Ⅰ 0.605 0.619 0.589 0.532 0.609 0.591
Ⅱ号No.Ⅱ 0.526 0.640 0.585 0.564 0.585 0.580
巴青垂穗披碱草
E.nutans G.
cv. Baqing		 0.557 0.589 0.579 0.605 0.658 0.598
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