Crops ›› 2016, Vol. 32 ›› Issue (4): 118-122.doi: 10.16035/j.issn.1001-7283.2016.04.019

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Effects of Water Stress on Physiological Characteristics of Different Genotypes of Triticale and Rye Seedlings

Bai Lirong,Shi Liran,Guo Xiaoli,Zhang Xiaona   

  1. Department of Life Science,Hengshui University,Hengshui 053000,Hebei,China
  • Received:2016-03-31 Revised:2016-06-30 Online:2016-08-15 Published:2018-08-26

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Abstract:

Eight varieties of triticale and one rye cultivars were treated with 20% polyethylene glycol 6000 simulated drought stress, and then seedling fresh weight, MDA content, organic osmoregulation substance such as proline, soluble sugar, soluble protein contents and three antioxidant enzymes (SOD, POD, CAT) activity were determined. The results showed that the seedling fresh weight of all varieties decreased, MDA content increased, proline content and soluble sugar content increased with water stress. But the soluble protein contents rised in some varieties (Jisi 2, Zhongxin 830, Dongmu 70, Zhongsi 237), while reduced in some other varieties(NTH1048, NTH1888, Zhongs828, Jinsong 49). The activity of SOD were increased, and the changes of activities of POD, CAT were different in all the 9 varieties. After a comprehensive analysis of the membership function, the drought resistance order of 9 varieties was as follows: Zhongxin 830>Zhongsi 237>Jisi 2>Jisi 1>Dongmu 70>Zhongsi 828>NTH1048>Jinsong 49>NTH1888.

Key words: Water stress, Triticale, Rye, Organic osmoregulation substance, Antioxidant enzymes, Degree of membership

Fig.1

Effects of water stress on the fresh weight of triticale and rye seedlings The different letters mean significant difference among the different treatment at 0.01 levels,the same below."

Fig.2

Effects of water stress on the MDA content of triticale and rye seedlings"

Table 1

Effects of water stress on the antioxidant enzymes activities of triticale and rye seedlings"

品种Varieties SOD(U/g FW) POD(U/g FW) CAT(U/g FW)
CK 20% PEG 增长率(%)
Increase rate		 CK 20% PEG 增长率(%)
Increase rate		 CK 20% PEG 增长率(%)
Increase rate
NTH1888 178AB 198A 11.0 9 380A 9 640A 2.8 81A 88A 8.8
NTH1048 209B 251A 20.3 13 465A 11 665B -13.4 83A 89A 7.2
中饲828 Zhongsi 828 210B 286A 36.1 12 945A 9 095B -29.7 90B 106A 17.8
劲松49 Jinsong 49 305AB 337A 10.6 20 035A 18 635AB -7.0 143A 130B -9.1
冀饲1号 Jisi 1 260B 330A 27.1 12 835A 9 130B -28.9 108A 114A 5.6
冀饲2号Jisi 2 212B 298A 40.4 13 275A 10 165B -23.4 82B 100A 22.0
中饲237 Zhongsi 237 252B 287A 13.8 10 900A 10 935A 0.3 113B 133A 17.7
中新830 Zhongxin 830 239B 327A 36.8 9 525B 12 444A 30.6 102B 120A 17.6
冬牧70 Dongmu 70 277B 319A 15.4 8 700B 17 350A 99.4 126A 106B -15.9

Table 2

The change of organic osmoregulation substance contents in leaf of different varieties triticale, rye seedlings on water stress"

品种
Varieties		 脯氨酸Proline(μg/g·FW) 可溶性糖Soluble sugar (mg/g·FW) 可溶性蛋白Soluble protein(mg/g·FW)
CK 20% PEG 增长率(%)Increasing rate CK 20% PEG 增长率(%)
Increase rate		 CK 20% PEG 增长率(%)
Increase rate
NTH1888 18.9B 223.9A 1 085 21.8B 27.1A 24.3 4.9A 3.5B -28.6
NTH1048 14.5B 178.3A 1 130 10.7B 29.0A 171.0 9.3A 5.8B -37.6
中饲828 Zhongsi 828 1.8B 44.8A 2 389 7.4B 21.9A 196.0 6.34A 4.9B -22.2
劲松49 Jinsong 49 10.9B 96.0A 781 15.4 B 18.7A 21.4 7.3A 6.5B -10.9
冀饲1号 Jisi 1 1.4B 24.1A 1 621 14.1B 24.9A 76.6 6.0A 6.0A 0
冀饲2号 Jisi 2 1.3B 19.9A 1 431 16.7B 26.3A 57.5 4.2B 5.9A 40.5
中饲237 Zhongsi 237 10.3B 72.3A 602 17.3B 25.9A 49.7 3.5B 6.4A 82.6
中新830 Zhongxin 830 8.6B 99.5A 1 057 19.8B 29.3A 47.9 4.8B 7.9A 64.6
冬牧70 Dongmu 70 14.6B 92.9A 536 15.1B 31.3A 107.3 3.9B 6.8A 74.4

Table 3

Membership degree of different varieties in water stress"

品种Varieties 鲜重
Fresh weight		 MDA 脯氨酸
Proline		 可溶性糖
Soluble sugar		 可溶性蛋白
Soluble protein		 SOD POD CAT 隶属度
Degree of membership
NTH1888 0.19 0.39 1.00 0.20 0.00 0.00 0.12 0.10 0.25
NTH1048 0.42 0.73 0.80 1.00 0.11 0.36 0.13 0.10 0.46
中饲828 Zhongsi 828 0.43 0.89 0.35 0.75 0.13 0.74 0.00 0.53 0.48
劲松49 Jinsong 49 0.00 0.42 0.23 0.00 0.32 0.67 0.39 0.43 0.31
冀饲1号 Jisi 1 0.66 0.70 0.05 0.38 0.34 0.88 0.00 0.46 0.43
冀饲2号 Jisi 2 1.00 1.00 0.00 0.34 0.55 0.87 0.05 0.49 0.54
中饲237 Zhongsi 237 0.74 1.00 0.09 0.29 0.88 0.47 0.16 1.00 0.58
中新830 Zhongxin 830 0.95 0.85 0.36 0.37 1.00 1.00 0.35 0.77 0.71
冬牧70 Dongmu 70 0.58 0.00 0.12 0.75 0.88 0.65 1.00 0.01 0.50
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