Crops ›› 2018, Vol. 34 ›› Issue (4): 131-137.doi: 10.16035/j.issn.1001-7283.2018.04.022

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Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings

Zhu Pengjin1,2,Pang Xinhua1,2,Liang Chun1,Tan Qinliang1,2,Yan Lin1,Zhou Quanguang1,2,Ou Kewei1,2   

  1. 1 Guangxi Subtropical Crops Research Institute, Nanning 530001, Guangxi, China
    2 Sugarcane Research Institute,Guangxi Agricultural Reclamation Bureau, Nanning 530001, Guangxi, China
  • Received:2018-03-12 Revised:2018-06-22 Online:2018-08-20 Published:2018-08-23

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Abstract:

The response of crops to low temperature stress is a complex process of physiological and biochemical changes. In order to breed the sugarcane varieties with strong cold resistance, stable yield and high sugar content, we selected four sugarcane varieties (lines) with different cold resistance abilities as the research materials based on the previous regional experiments. In this study, through measuring and analyzing the responses of the metabolism of reactive oxygen species (ROS) and the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) of different sugarcane varieties (lines) to low temperature, it was found that the ROS in seedling leaves increased, and the activities of antioxidant enzymes (SOD, CAT and APX) increased at a high rate within a certain time accordingly and scavenged the ROS generated during the low temperature stress, showing a decrease-increase-decrease fluctuation. However, the activities of antioxidant enzymes to cold stress varied with sugarcane varieties (lines). In addition, the relative electrical conductivity and the malondialdehyde (MDA) content in leaves also increased in volatility as the cold stress time prolonged. According to the changes of the relative conductivity and MDA, the sensitivities to low temperature stress of different sugarcane varieties (lines) could be deduced as ROC22 > FN39 > GR2 > GT28.

Key words: Chilling stress, Sugarcane, Antioxidant enzymes, Reactive oxygen species

Fig.1

Effects of low-temperature stress on the superoxide anion metabolize and hydrogen peroxide content of sugarcane seedling leaves Different letters indicate significant difference (P<0.05) according to ANOVA analysis. The vertical bar indicates the mean±SE. CK: the control, normal temperature, T: 4℃ treatment. The same below"

Fig.2

Effects of low-temperature stress on the relative conductivity and MDA content of sugarcane seedling leaves"

Fig.3

Effects of low temperature stress on antioxidant enzymes of sugarcane seedling leaves"

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