Crops ›› 2022, Vol. 38 ›› Issue (2): 143-152.doi: 10.16035/j.issn.1001-7283.2022.02.020

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Effects of Calcium by Foliage Spraying on Photosynthesis Physiology Characteristics of Flue-Cured Tobacco under Light Stress

Yun Fei1(), Ren Tianbao1,*(), Yin Quanyu1, Jin Shuangzhen2, Zheng Cong3, Jin Lei4, Li Jingjing4, Liu Guoshun1, Yang Xitian5,*()   

  1. 1College of Tobacco Science, Henan Agricultural University/Henan Engineering Research Center for Biochar, Zhengzhou 450002, Henan, China
    2Dali Municipal Branch of Yunnan Tobacco Company, Dali 671000, Yunnan, China
    3Nanping Municipal Branch of Fujian Tobacco Company, Nanping 353100, Fujian, China
    4Jiyuan Municipal Branch of Henan Tobacco Company, Jiyuan 454650, Henan, China
    5College of Forestry, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2021-08-25 Revised:2021-10-09 Online:2022-04-15 Published:2022-04-24
  • Contact: Ren Tianbao,Yang Xitian E-mail:yunfeifei55@126.com;tianbao1016@vip.126.com;xitianyang@yahoo.com.cn

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Abstract:

The effects of the exogenous calcium on the photosynthetic pigments, gas exchange parameters, antioxidant enzyme activities, osmoregulation substances and dry matter accumulation of flue-cured tobacco were investigated with flue-cured tobacco variety K326 as experimental material. The soil-culture pot experiment was carried out under three light intensities in the artificial climate chamber in order to study the regulating effects of the exogenous calcium salts on the photosynthetic physiological characteristics and antioxidant system of flue-cured tobacco under light stress. The results showed that the contents of chlorophyll (Chla+b) and carotenoid (Car) in flue-cured tobacco leaves under different light intensities were increased by the application of exogenous calcium, and the highest values of Chla+b and Car respectively appeared in 400-500μmol/(m2·s) low light treatment (L1) and 1500-1800μmol/(m2·s) high light treatment (L3). With the increase of light intensity, the net photosynthetic rate (Pn) was increased, but the light energy utilization efficiency (LUE) of L1 treatment was the highest. The values of Pn, transpiration rate (Tr), stomatal conductance (Gs) and LUE were significantly increased by spraying exogenous calcium on flue-cured tobacco leaves,the increase proportion of LUE was higher under L1 and L3 treatments, increased by 3.83%-5.63% and 4.65%-7.75% compared with the control, respectively. The superoxide dismutase and peroxidase activities, the soluble sugar and dry matter accumulation contents were the highest under the condition of L3 treatment with exogenous calcium application, but the content of malonaldehyde was the lowest. Consequently, in order to protect the PSII reaction center under light stress, the exogenous calcium application enhanced the active oxygen scavenging enzyme activity and osmotic adjustment substance content, the photosynthetic efficiency of flue-cured tobacco could be effectively improved so that the photosynthetic carbon assimilation products could be promoted.

Key words: Calcium, Light stress, Flue-cured tobacco (Nicotinna tobacum L.), Photosynthesis physiology characteristics

Fig.1

Effects of calcium by foliage spraying on the photosynthetic pigment contentes of flue-cured tobacco leaves under light stress Different lowercase letters indicate significant difference of light intensity (outside the brackets) and nitrogen supply (inside the brackets) at P < 0.05 level, the same below"

Fig.2

Effects of calcium by foliage spraying on gas exchange indexes of flue-cured tobacco leaves under light stress"

Fig.3

Effects of calcium by foliage spraying on LUE of flue-cured tobacco under light stress"

Fig.4

Effects of calcium by foliage spray on the activities of antioxidase in flue-cured tobacco leaves under light stress"

Fig.5

Effects of calcium by foliage spraying on the contents of MDA and SS in flue-cured tobacco leaves under light stress"

Fig.6

Effects of calcium by foliage spraying on the content of dry matter accumulation in flue-cured tobacco leaves under light stress"

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