Crops ›› 2025, Vol. 41 ›› Issue (5): 86-92.doi: 10.16035/j.issn.1001-7283.2025.05.012

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Effects of Biological Sulfur Regulator on the Yield, Quality of Alfalfa and Soil Physicochemical Properties in Saline-Alkali Land

Jia Yanli1(), Zhang Hongfa2, Liu Guixia3, Huang Sufang1, Qu Xinyue1, Yue Mingqiang1, Han Jiating1, Xu Yupeng1, Liu Qingsong1()   

  1. 1 Cangzhou Academy of Agricultural and Forestry Sciences, Cangzhou 061001, Hebei, China
    2 Cangzhou Livestock Technology Promotion Station, Cangzhou 061001, Hebei, China
    3 College of Life Sciences, Hebei University, Baoding 071002, Hebei, China
  • Received:2024-09-23 Revised:2024-12-02 Online:2025-10-15 Published:2025-10-21

Abstract:

To study the effects of different application rates of biological sulfur regulators on the yield, quality, and soil physicochemical properties of alfalfa in saline-alkali land in eastern Hebei Province, alfalfa fields were applied at application levels of 3, 6, 9, and 12 t/ha on November 25, 2023. The results showed that with the increasing of the application amount of biological sulfur regulator, the greening time of alfalfa gradually delayed, and the biological sulfur regulator could prolong the overwintering time of alfalfa. Biological sulfur regulator could significantly improve the plant height, grass yield, and quality traits of alfalfa. The highest plant height was achieved at a rate of 9 t/ha, reached 56.9 cm. Alfalfa stem diameter, branching number, and leaf-stem ratio all performed best at a rate of 9 t/ha. Under the application rate of 9 t/ha, the yields of fresh grass and hay in the first cut were 29 254.5 kg/ha and 7045.2 kg/ha, respectively. At the same time, the crude protein and relative feeding value reached 23.23% and 179.40, respectively. The contents of crude fat, calcium, and phosphorus were 2.26%, 1.67% and 0.42%, respectively, which were significantly higher than other treatments. After the experiment, the soil pH and salt content were the lowest at a dosage of 12 t/ha, which were 8.97 and 0.285%, respectively; the organic matter, microbial carbon and microbial nitrogen contents were the highest at 7.45 g/kg, 61.02 mg/kg and 6.61 mg/kg, respectively, but there were no significant difference compared to the 9 t/ha dosage. Therefore, the optimal application rate of biological sulfur regulator for alfalfa in saline-alkali land was 9 t/ha.

Key words: Biological sulfur regulator, Saline-alkali land, Alfalfa, Yield, Quality, Soil properties

Table 1

Returning to green time of alfalfa under different application rates of biological sulfur regulators"

处理
Treatment
返青时间(月-日)
Returning to green time (month-day)
CK 03-12
S3 03-12
S6 03-13
S9 03-15
S12 03-16

Table 2

Comparison of alfalfa growth trait under different application rates of biological sulfur regulators"

处理
Treatment
株高
Plant
height (cm)
茎粗
Stem
diameter (mm)
分枝数
Branch
number
叶茎比
Leaf-stem
ratio
CK 54.9±2.1c 1.91±0.01b 12.1±0.3b 0.852±0.009b
S3 55.6±2.3b 1.94±0.03b 12.5±0.4b 0.855±0.012b
S6 56.6±1.9a 2.02±0.03a 13.9±0.2a 0.902±0.011a
S9 56.9±2.4a 2.05±0.02a 14.2±0.3a 0.911±0.014a
S12 55.2±1.7c 1.91±0.01b 12.6±0.1b 0.857±0.006b

Table 3

Comparison of fresh grass yield and hay yield of alfalfa under different application rates of biological sulfur regulators kg/hm2"

处理Treatment 鲜草产量Fresh grass yield 干草产量Hay yield
CK 23 131.5±133.2d 5731.3±64.5d
S3 26 533.5±149.4c 6168.0±67.7c
S6 27 603.0±134.1b 6538.5±72.9b
S9 29 254.5±166.8a 7045.2±77.1a
S12 23 410.5±120.9d 5841.0±53.2d

Table 4

Comparison of alfalfa quality under different application rates of biological sulfur regulators"

处理
Treatment
干物质
Dry matter
(%)
粗蛋白
Crude protein
(%)
粗脂肪
Crude fat
(%)
酸性洗涤纤维
Acid detergent
fiber (%)
中性洗涤纤维
Neutral detergent
fiber (%)
相对饲喂价值
Relative
feeding value

Ca (%)

P (%)
CK 92.15±0.45a 19.88±1.44d 1.98±0.02c 26.37±2.12a 39.52±2.33a 160.90±6.34e 1.49±0.04b 0.35±0.01b
S3 92.09±0.31a 21.28±1.09c 2.02±0.03c 25.88±2.09b 38.59±2.01b 165.70±7.29d 1.52±0.04b 0.36±0.00b
S6 92.34±0.22a 22.39±1.22b 2.24±0.07a 25.12±1.91c 36.71±1.99d 175.69±1.55b 1.64±0.05a 0.41±0.01a
S9 92.17±0.25a 23.23±1.34a 2.26±0.06a 24.40±1.56d 36.24±2.43e 179.40±1.91a 1.67±0.03a 0.42±0.02a
S12 92.20±0.29a 22.11±1.87b 2.14±0.02b 25.45±1.68c 37.46±2.81c 171.53±6.31c 1.55±0.02b 0.36±0.00b

Table 5

Changes in soil pH, salt content, and organic matter content of different application rates of biological sulfur regulators"

处理
Treatment
pH 含盐量Salt content (%) 有机质含量Organic matter content (g/kg)
试验前
Before experiment
试验后
After experiment
试验前
Before experiment
试验后
After experiment
试验前
Before experiment
试验后
After experiment
CK 9.13 9.13±0.12a 0.314 0.321±0.009a 7.39 7.24±0.12d
S3 9.13 9.11±0.09a 0.314 0.311±0.011b 7.39 7.33±0.09c
S6 9.13 9.07±0.11b 0.314 0.304±0.007c 7.39 7.38±0.11b
S9 9.13 9.07±0.04b 0.314 0.289±0.014d 7.39 7.44±0.14a
S12 9.13 8.97±0.05c 0.314 0.285±0.012d 7.39 7.45±0.10a

Table 6

Changes in soil microbial carbon and nitrogen of different application rates of biological sulfur regulators mg/kg"

处理
Treatment
微生物量碳Microbial carbon 微生物量氮Microbial nitrogen
试验前Before experiment 试验后After experiment 试验前Before experiment 试验后After experiment
CK 60.12 58.39±1.51c 6.32 5.94±0.12d
S3 60.12 60.44±2.02b 6.32 6.12±0.20c
S6 60.12 60.57±1.45b 6.32 6.34±0.15b
S9 60.12 60.99±1.79a 6.32 6.55±0.14a
S12 60.12 61.02±1.62a 6.32 6.61±0.14a

Table 7

Comparison of membership function values for various trait indicators of alfalfa of different application rates of biological sulfur regulators"

处理
Treatment
株高
Plant height
茎粗
Stem diameter
分枝数
Number of branches
叶茎比
Leaf-stem ratio
鲜草产量
Fresh grass yield
干草产量
Hay yield
粗蛋白
Crude protein
粗脂肪
Crude fat

Ca

P
CK 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
S3 0.350 0.214 0.190 0.051 0.556 0.332 0.418 0.143 0.167 0.143
S6 0.850 0.786 0.857 0.847 0.730 0.614 0.749 0.929 0.833 0.857
S9 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
S12 0.150 0.000 0.238 0.085 0.046 0.083 0.018 0.571 0.333 0.143
CK 1.000 1.000 0.000 1.000 1.000 0.000 0.000 0.000 0.222 5
S3 0.751 0.716 0.259 0.875 0.722 0.429 0.779 0.269 0.409 3
S6 0.365 0.143 0.799 0.625 0.528 0.667 0.829 0.597 0.700 2
S9 0.000 0.000 1.000 0.625 0.111 0.952 0.989 0.910 0.810 1
S12 0.533 0.372 0.005 0.000 0.000 1.000 1.000 1.000 0.310 4
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