Crops ›› 2021, Vol. 37 ›› Issue (3): 149-154.doi: 10.16035/j.issn.1001-7283.2021.03.022

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Isolation, Screening, Phosphorus Solubilization and Plant Growth-Promoting Characteristics of Rhizosphere Halotolerant Fungi

Tan Haixia1(), Li Liyan2,3(), Du Yinghui2,3, Sun Shanshan2,3, Wang Lianlong1, Dong Xue1, Wang Yuxi1, Zhang Ying1   

  1. 1Hebei University of Environmental Engineering, Qinhuangdao 066000, Hebei, China
    2Leading Biological and Agricultural Limited Company, Qinhuangdao 066000, Hebei, China
    3Hebei Agricultural Biotechnology Technology Innovation Center, Qinhuangdao 066000, Hebei, China
  • Received:2020-07-06 Revised:2020-10-20 Online:2021-06-15 Published:2021-06-22

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Abstract:

To obtain high-efficiency halotolerant phosphorus strains and provide high-quality bacteria fertilizer species resources for the development of saline soil, phosphate solubilizing fungus were isolated and screened from the rhizosphere soil of coastal halophytes.The results showed that five inorganic phosphorus strains and three organic phosphate solubilizing strains were isolated in rhizosphere soil of three halophytes from Beidaihe wetland and Caofeidian wetland, named C1-C7, G1, grown at 10% sodium chloride medium. The D/d values of the eight fungus ranged from 1.05 to 1.81. The effective phosphorus content was 26.61-4415.00mg/L in the inorganic phosphorus strains, which was 0.80-4371.10mg/L higher than CK; the effective phosphorus content was 1.96-5.79mg/L in organic phosphate solubilizing strains, which was -3.40-0.20mg/L higher than CK. There was a weak correlation between effective phosphorus content and D/d. The strain C1 could produce 4397.70mg/L available phosphate, meanwhile, C1 had the ability to promote plant growth with IAA secretion of 33.07mg/L. The strain C1 was identified as Penicillium oxalicum, and the effective phosphorus content between strains showed significant difference. Strain C1 was a highly effective phosphate-dissolving halotolerant fungi with growth-promoting activity. It could be considered as a key strain for the development of microbial fertilizer in saline-alkali land which had great potential to improve phosphorus utilization rate in moderate-to-severe saline soil by plant-microorganism.

Key words: Rhizosphere soil, Halotolerant fungi, Isolation, Phosphate solubilizing fungi, Growth-promoting

Table 1

Physical and chemical properties of the soil in the sampling sites"

采样地点
Sampling
site		 碱解氮
Alkaline nitrogen
(mg/kg)		 有效磷
Available phosphorus
(mg/kg)		 速效钾
Quick-acting
potassium (mg/kg)		 有机质
Organic matter
(g/kg)		 水溶性盐
Water soluble
salt (‰)		 pH
北戴河湿地Beidaihe wetland 33.21 14.35 271.00 5.95 4.50 8.56
曹妃甸湿地Caofeidian wetland 31.50 34.63 753.38 11.85 3.10 8.44

Table 2

Quantitative characteristics of halotolerant fungi in different plant root soils"

土壤来源
Soil source		 真菌
Fungus
(×103cfu/g)		 耐盐优势菌株数
Number of halotolerant fungi		 采样地点
Sampling
site
盐地碱蓬Suaeda salsa 4.52 2 北戴河湿地
盐角草
Salicornia europaea		 5.05 3 北戴河湿地
碱蓬Suaeda glauca 4.00 5 曹妃甸湿地
盐地碱蓬Suaeda salsa 61.25 6 曹妃甸湿地

Table 3

Phosphate-solubilizing capacity of inorganic phosphate solubilizing fungi"

菌株Fungus D (mm) d (mm) D/d 有效磷含量Available phosphorus (mg/L) 来源Source 采样地Sampling site
CK1 - - - 26.60±0.26e - -
C1 19.0±2.0 12.0±2.0 1.60±0.21 4397.70±17.60a 碱蓬 曹妃甸湿地
C2 18.3±1.5 16.3±2.1 1.13±0.08 27.40±0.79e 碱蓬 曹妃甸湿地
C3 18.0±2.0 15.3±3.2 1.19±0.12 1715.60±0.45c 盐地碱蓬 曹妃甸湿地
C4 15.0±2.7 10.3±0.6 1.46±0.30 1803.60±5.73b 盐地碱蓬 曹妃甸湿地
G1 5.3±1.5 3.3±0.5 1.58±0.22 1662.30±0.45d 盐角草 北戴河湿地

Table 4

Phosphate-solubilizing capacity of organic phosphate solubilizing fungi"

菌株Fungi D (mm) d (mm) D/d 有效磷含量Available phosphorus (mg/L) 来源Source 采样地Sampling site
CK2 - - - 5.50±0.08a - -
C5 36.7±1.2 32.0±2.0 1.15±0.02 5.70±0.09a 碱蓬 曹妃甸湿地
C6 45.3±2.5 34.0±4.4 1.34±0.10 2.10±0.14c 盐地碱蓬 曹妃甸湿地
C7 42.7±1.5 24.3±1.5 1.76±0.16 4.90±0.05b 盐地碱蓬 曹妃甸湿地

Fig.1

The correlation between D/d of inorganic phosphorus fungi (a) and organic phosphate fungi (b) and soluble phosphorus content"

Table 5

IAA secretion of halotolerant fungi"

菌株编号
Fungi number		 显色反应
Color rendering
reaction		 IAA分泌量
IAA secretion
amount (mg/L)
C1 +++ 33.07±0.55a
C2 ++ 20.87±0.56b
C3 ++ 17.44±1.09b
C4 + 6.64±0.22c
C5 ++ 15.67±0.90b
C6 - -
C7 + 7.23±0.16c
G1 - -

Fig.3

Phylogenetic tree of strain C1 based on ITS gene sequences similarity GenBank accession numbers of aligned sequences are shown in the brackets. The bootstrap values are shown at the node. The length of the scale is 0.0005 nucleotide replacement rate"

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