Crops ›› 2020, Vol. 36 ›› Issue (2): 176-181.doi: 10.16035/j.issn.1001-7283.2020.02.027

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Isolation and Identification of IAA - Producing Rhizobacteria and Its Effects on Seed Germination and Seedling Growth of Tobacco

Chen Yue,Li Hulin,Zhu Shimiao,Yan Han,Lang Bin,Ji Wenxiu   

  1. College of Agriculture, Yanbian University, Yanji 133002, Jilin, China
  • Received:2019-09-25 Revised:2019-12-28 Online:2020-04-15 Published:2020-04-13

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Abstract:

In order to obtain the high-quality bacteria resources and to explore their effects on the seed germination and seedling growth of tobacco, we isolated and screened IAA-producing strains from rhizosphere soil of tobacco in Yanbian area and carried out 16S rRNA sequencing identification, as well as investigated the effects of the single and mixed bacteria on the germination and seedling growth of tobacco. The results showed that both Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 produced more IAA than other strains, and the optimal culture time of m-53, m-60 and their mixture for growth were 24, 48 and 48h, and their corresponding IAA yields were 98, 41 and 141mg/L, respectively. Compared with the control, both single and mixed bacteria could promote seed germination and seedling growth of tobacco, especially the mixed bacteria had the best promotion effect. Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 can be applied separately or in combination as an alternative resource for microbial fertilizers of tobacco.

Key words: Tobacco, Plant growth-promoting rhizobacteria, Indole acetic acid, Promoting effect

Table 1

The ability of IAA production of rhizosphere bacteria from tobacco mg/L"

编号No. IAA含量IAA content 编号No. IAA含量IAA content 编号No. IAA含量IAA content
m-53 43.06±3.17a m-125 19.14±0.79cdefg m-113 15.47±0.88fghi
m-60 41.37±5.56a m-10 18.42±1.47cdefgh m-109 15.47±0.30fghi
m-75 27.95±2.79b m-7 18.28±1.54cdefgh m-8 15.37±1.48fghi
m-118 25.81±2.64bc m-74 17.89±0.28cdefgh m-97 14.70±1.07fghi
m-48 24.45±1.77bcd m-119 16.36±1.55defghi m-64 10.86±2.54ghi
m-72 24.36±1.68bcd m-117 16.09±2.35efghi m-129 10.34±0.76hi
m-89 22.28±2.59bcdef m-90 16.06±0.30efghi m-130 10.34±0.33i
m-65 20.23±2.09bcdef m-102 15.75±1.01efghi m-4 9.31±2.04i
m-66 19.73±0.89cdef m-122 15.59±1.99fghi m-24 8.31±0.17i

Table 2

Comparison of 16S rRNA sequence and GenBank-BLAST of bacteria"

编号
No.		 相似菌属
Similar genera		 相似度
Similarity (%)		 登录号
Login ID
m-53 鞘氨醇杆菌属Sphingobacterium 100 KC009697.1
m-60 咸海鲜球菌属Jeotgalicoccus 100 KT989860.1

Fig.1

Morphology of m-53 and m-60 strains"

Fig.2

The results of antagonistic reaction between m-53 and m-60 strains"

Fig.3

The effects of culture time on the growth of strain and its combination"

Fig.4

The effects of culture time on IAA production ability of strain and its combination"

Table 3

The effects of bacteria on germination indexes of tobacco seeds"

处理
Treatment		 发芽势
Germination
potential (%)		 发芽率
Germination
rate (%)		 发芽指数
Germination
index		 活力指数
Vitality
index
CK 56.67±8.74c 86.00±6.66a 0.90±0.02b 2.41±0.06c
m-53 66.00±2.65b 89.67±1.53a 0.95±0.02b 2.64±0.06b
m-60 66.00±8.19b 87.00±4.00a 0.95±0.06b 2.56±0.15bc
m-53 + m-60 72.67±2.08a 88.67±4.51a 1.12±0.02a 3.23±0.06a

Table 4

The effects of bacteria on agronomic traits of tobacco seedlings"

处理
Treatment		 株高(cm)
Plant height		 根长(cm)
Root length		 最大叶长(cm)
Maximum leaf length		 最大叶宽(cm)
Maximum leaf width		 茎围(mm)
Stem circumference		 叶片数
Number of leaves
CK 2.34±0.11c 2.06±0.38c 1.36±0.05c 1.24±0.05c 0.050±0.007c 3.02±0.04c
m-53 3.26±0.11b 2.54±0.09b 2.08±0.08b 1.66±0.05b 0.084±0.005b 3.16±0.11ab
m-60 2.28±0.13c 1.76±0.38d 1.36±0.05c 1.16±0.05c 0.050±0.006c 3.04±0.05c
m-53 + m-60 3.66±0.09a 4.42±0.43a 2.32±0.28a 1.82±0.04a 0.118±0.026a 3.20±0.07a

Fig.5

The effects of growth-promoting bacteria on the growth of tobacco seedlings"

Table 5

The effects of bacteria on biomass of tobacco seedlings"

处理
Treatment		 地上部Shoot 地下部Root 根冠比Root-shoot ratio
鲜重Fresh weight (mg) 干重Dry weight(mg) 鲜重Fresh weight (mg) 干重Dry weight (mg) 鲜重Fresh weight 干重Dry weight
CK 46.00±1.00c 2.67±0.41c 3.30±0.07c 0.28±0.10c 0.072±0.022b 0.109±0.045b
m-53 91.20±1.64b 5.99±0.17b 11.10±0.20b 0.92±0.05b 0.122±0.002a 0.154±0.005ab
m-60 41.40±0.55d 1.59±0.01d 3.00±0.22c 0.29±0.13c 0.072±0.029b 0.181±0.071a
m-53 + m-60 106.20±4.21a 7.02±0.36a 14.24±1.23a 1.28±0.19a 0.134±0.011a 0.184±0.032a
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