Crops ›› 2022, Vol. 38 ›› Issue (5): 22-26.doi: 10.16035/j.issn.1001-7283.2022.05.003

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Convergence Improvement of Aroma Genes and Rice Blast Resistance Genes in Kongyu 131

Li Ruiqi1(), Bi Haoran2(), Jiang Gonghao2(), Duan Haiyan1()   

  1. 1College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2021-06-20 Revised:2021-11-09 Online:2022-10-15 Published:2022-10-19

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Abstract:

Despite having Pik and Pia rice blast resistance genes, Kongyu 131 nonetheless lost its blast resistance in production under the current rice farming practises in Heilongjiang province. There is also an urgent need for focused improvement because the existing elite variety lacks scent, one of its advantageous traits. Genetic enhancement is therefore necessary to strengthen blast resistance and introduce the fragrance gene. In order to obtain the outcomes of genetic improvement of aroma characteristics and rice blast resistance in Kongyu 131, we now reported the introduction of fragrance gene Osbadh2 (fgr) and blast resistance genes, Pi1, Pi2, and Pi9, into Kongyu 131. We have obtained three kinds of two-gene combinations (Pi1+fgr, Pi2+fgr and Pi9+fgr) showing strong resistance to rice blast, together with rice aroma quality providing foundation for Kongyu 131 further improvement.

Key words: Kongyu 131, Fragrance gene, Blast resistant gene, Pyramiding breeding

Table 1

Identification pyramided genes of F2 generation of double genes polymerization plants"

聚合基因
Pyramided
gene		 引物
Primer		 样品数量
Number
of samples		 单基因纯合单株数
Number of monogenic
homozygosity plants		 双基因纯合单株数
Number of double gene
homozygosity plants
Pi1+fgr BadF4-R4/RM27322 200 31(Pi1) 6
Pi2+fgr BadF4-R4/L11 200 27(fgr) 7
Pi9+fgr BadF4-R4/L7 200 27(Pi9) 8

Fig.1

The PCR identificationto of F2 generation of double-gene polymerization individuals (a) amplification results of Pi1 and fgr in KY(Pi1+fgr); (b) amplification results of Pi2 and fgr in KY(Pi2+fgr); (c) amplification results of Pi9 and fgr in KY(Pi9+fgr). D is Inari"

Table 2

Aroma quality appraisal of F3 generation of double genes polymerization plants"

基因组合
Gene
combination		 改良系空育131所占的比例
Proportion of improved
Kongyu 131 (%)		 香味程度
Degree
of scent
Pi1+fgr 0 无香
20 微弱的香味
40 较香
60 香
80 浓香
100 浓香
Pi2+fgr 0 无香
20 微弱的香味
40 较香
60 香
80 浓香
100 浓香
Pi9+fgr 0 无香
20 微弱的香味
40 较香
60 香
80 浓香
100 浓香

Table 3

Rice blast resistance identification of F3 generation of double genes polymerization plants"

抗性等级
Resistance grade		 基因组合Gene combination
Pi1+fgr Pi2+fgr Pi9+fgr CK（KY131）
稻瘟病Rice blast 3 3 4 6

Fig.2

Analysis results of gene chip in double gene polymerization individuals Red area, blue area and transparent area are purely differential loci, heterozygous differential loci, and loci with the same genetic background in KY(Pi1+fgr), KY(Pi2+fgr), KY(Pi9+fgr) and KY131, respectively"

Table 4

Investigation of agronomic traits of F3 generation in double-gene polymerization plants"

基因组合
Gene combination		 分蘖数
Number
of tillers		 穗长
Ear length
(cm)		 单穗粒数
Number of grains
per panicle		 单株粒重
Grain weight
per plant (g)		 千粒重
1000-grain
weight (g)		 结实率
Seed-setting
rate (%)
KY131（CK） 28.50±6.26 14.78±1.12 87.30±10.42 45.07±8.36 25.55±1.56 90.90±1.31
KY（Pi1+fgr） 31.70±3.37 14.97±0.41 91.40±9.40 45.71±6.66 24.65±0.71 90.12±1.52
KY（Pi1+fgr） 30.10±3.63 14.98±0.39 93.10±10.37 42.04±6.11 24.60±0.84 90.29±1.49
KY（Pi2+fgr） 28.80±2.97 15.17±0.41 90.20±10.12 42.78±4.74 24.72±1.00 90.62±2.22
KY（Pi2+fgr） 28.70±2.91 15.11±0.47 88.80±10.41 42.31±4.76 25.03±0.97 91.04±2.42
KY（Pi9+fgr） 31.10±4.70 15.19±0.62 90.10±11.69 46.52±8.65 24.41±0.90 90.65±2.35
KY（Pi9+fgr） 31.70±5.50 14.99±0.60 91.00±11.76 43.88±7.94 24.06±0.90 90.94±2.36

Fig.3

Agronomic traits of F3 generation of double genes polymerization plants"

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