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作物杂志,2018, 第6期: 116–123 doi: 10.16035/j.issn.1001-7283.2018.06.018

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

外源过氧化氢对烟草花芽分化的影响初探

王宁,张静,黄进勇,史团省,杜建,岳彩鹏   

  1. 郑州大学生命科学学院,450001,河南郑州
  • 收稿日期:2018-09-30 出版日期:2018-12-15 发布日期:2018-12-06
  • 作者简介:王宁,硕士,主要从事植物生理研究;张静为共同第一作者,硕士,主要从事植物生理研究
  • 基金资助:
    河南省基础与前沿技术研究计划项目(162300410176)

Effects of Exogenous Hydrogen Peroxide on Floral Bud Differentiation in Tobacco

Wang Ning,Zhang Jing,Huang Jinyong,Shi Tuansheng,Du Jian,Yue Caipeng   

  1. School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China
  • Received:2018-09-30 Online:2018-12-15 Published:2018-12-06

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摘要：

通过盆栽试验,对外源H2O2处理的烟草活性氧代谢及烟草花芽分化情况进行了初步研究。结果表明：（1）外源H2O2处理显著影响了烟草体内活性氧代谢,H2O2和O2 -含量迅速增加;保护酶POD和CAT活性迅速被激活,SOD的激活滞后于POD和CAT。喷施H2O2处理的中后期H2O2的含量下降。（2）外源H2O2胁迫改变了开花基因的表达、现蕾时烟草叶片数以及花芽的发育进程。5和15d的外源H2O2处理明显抑制了烟草FLC基因的表达,促进了LFY基因的表达,促使烟草花发育提前。试验结果表明烟草体内活性氧平衡状态的变化影响烟草的花发育进程。

关键词: 烟草, 外源过氧化氢, 活性氧, 花芽分化

Abstract:

The active oxygen metabolism and flower bud differentiation in tobacco were studied by spraying exogenous H2O2 via pot experiments. Results showed that: (1) The active oxygen metabolism was significantly changed in tobacco by spraying H2O2. After spraying H2O2, the content of H2O2 and O2 _ was rapidly increased in tobacco, the activities of POD and CAT were rapidly activated followed by the activation of SOD. In the middle and latter period of spraying, the content of O2 _ was high, while the content of H2O2 was significantly decreased. (2) The expressions of the floral development genes were affected, the number of leaves in squaring stage was altered and the process of flower bud development was modified by spraying of H2O2. The expression of FLC gene was significantly inhibited while the expression of LFY gene was significantly promoted in tobaccos which were sprayed H2O2 at 5 and 15 consecutive days. Floral bud differentiation of tobaccos progressed earlier under 5 to 25 consecutive days spray of H2O2. The flowering transition was affected by the change of active oxygen equilibrium in tobacco.

Key words: Tobacco, Exogenous hydrogen peroxide, Reactive oxygen species, Floral bud differentiation

图1

外源H2O2处理烟草O2-含量的快速响应变化 *：0.05水平差异显著;**：0.01水平差异极显著;下同"

图2

长时间外源H2O2处理烟草O2-含量的变化"

图3

外源H2O2处理烟草H2O2含量的快速响应变化"

图4

长时间外源H2O2处理烟草H2O2含量的变化"

图5

外源H2O2处理POD活性的快速响应变化"

图6

长时间外源H2O2处理烟草POD活性的变化"

图7

外源H2O2处理烟草CAT活性的快速响应变化"

图8

长时间外源H2O2处理烟草体内CAT活性的变化"

图9

外源H2O2处理烟草SOD活性的快速响应变化"

图10

长时间外源H2O2处理烟草体内SOD活性的变化"

图11

外源H2O2对基因FLC表达量的影响"

图12

外源H2O2对基因LFY表达量的影响"

图13

外源H2O2条件下花芽分化情况(10×5）"

图14

外源H2O2处理烟草现蕾时叶片数"

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