Table 1

The random primers used in amplification and their sequences (5′-3′)"

Fig.1

DNA extraction in industrial hemp M, DNA marker is 1kb plus DNA ladder; 1-2, The female of HM1; 3-4, The male of HM1; 5, The female of SH2; 6, The male of SH2; 7, The female of WD2; 8, The male of WD2; 9, The female of LJ; 10, The male of LJ; 11, The female of BQ; 12, The male of BQ; 13, The female of LB; 14, The male of LB; 15-16, The leaves of QM1 at seedling stage; 17-18, The leaves of JM1 at seedling stage; 19, Monoecism industrial hemp USO-14"

Fig.2

PCR amplification with the DNA pools of female and male plants in industrial hemp HM1 using primer OPV-08 Arrow indicate that there is a male specific fragment at>750bp; DNA marker is D2000 DNA ladder"

Fig.3

Gel recovery of male specific DNA fragments"

Fig.4

The nucleotide sequences of male specific DNA fragments"

Fig.5

SCAR molecular markers of HM1 male DNA marker is D2000 DNA ladder"

Fig.6

Detection of male and female plants in six dioecious industrial hemp by SCAR molecular markers 1-6 are HM1, SH2, WD2, LJ, BQ, LB, respectively; DNA marker is D2000 DNA ladder"

Fig.7

Sex detection of dioecious industrial hemp at seedling stage by SCAR molecular markers DNA marker is D2000 DNA ladder"

Fig.8

Analysis of SCAR molecular markers in monoecism industrial hemp DNA marker is D2000 DNA ladder"