Crops ›› 2017, Vol. 33 ›› Issue (6): 30-36.doi: 10.16035/j.issn.1001-7283.2017.06.006

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Genetic Difference Analysis and Construction of SSR Fingerprinting Database for F-Type Wheat Male Sterile Line and Restorer Lines

Liu Lihua1,Yuan Shaohua1,Feng Shuying2,Pang Binshuang1,Li Hongbo1,Liu Yangna1,Zhang Liping1,Zhao Changping1   

  1. 1Beijing Engineering and Technique Research Center for Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences,The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat,Beijing 100097,China
    2Blue and Red Hybrid Wheat Research Center,Yuncheng 044000,Shanxi,China
  • Received:2017-07-04 Revised:2017-09-22 Online:2017-12-15 Published:2018-08-26
  • Contact: Liping Zhang,Changping Zhao

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Abstract:

In order to protect wheat variety and provide theoretical reference for the selection of hybrid wheat combinations, twenty-one core SSRs covering the entire wheat genome were used to construct fingerprinting database and analyze genetic difference for F-type wheat male sterile line and 442 restorer lines, based on fluorescence capillary electrophoresis detection platforms and SSR fingerprint database management system. Two hundred and fourteen allelic variations were detected with an average allele number of 10.76. The average of polymorphism information content (PIC) and gene diversity values were determined to be 0.70 and 0.73, respectively. The resolution of twenty-one core SSR primers was 100%. The unique DNA fingerprinting of each material was obtained, which provided technical support for the protection of variety rights. The genetic distance between the restorer lines was 0.014-0.952 with an average allele number of 0.723, and the genetic distance greater than 0.500 reached 95%, which indicated that the genetic diversity among the restorer lines was more abundant. The genetic distance between sterile line and restorer lines was 0.300-0.952, with an average allele number of 0.681, and the restorer lines accounted for 93% with genetic distance greater than 0.500, which showed that there was a great genetic difference between sterile line and restorer lines and it was beneficial to select the restorer line and sterile line configuration with large genetic distance.

Key words: Wheat, F-type male sterile line, Restorer, SSR markers, Fingerprinting database, Genetic difference

Fig.1

Eight peak patterns detected on locus gwm155 using DNA analyzer Vertical axis indicates the intensity of fluorescent signals. 127, 130, 142, 144, 146, 148, 150, and 152 on abscissa axis indicate allele name"

Table 1

Polymorphism evaluation of 21 SSR core primers"

引物编号
Primer No.		 引物名称
Primer name		 等位变异数
Allele number		 PIC 基因多样性
Gene diversity
WP01 cwm65 9 0.68 0.73
WP02 barc80 6 0.61 0.66
WP03 cfd72 10 0.65 0.70
WP04 gwm294 12 0.72 0.74
WP05 gwm429 12 0.69 0.73
WP06 gwm261 10 0.68 0.71
WP07 gwm155 8 0.75 0.78
WP08 gwm285 14 0.83 0.85
WP09 gdm72 8 0.72 0.75
WP10 gwm610 6 0.56 0.61
WP11 ksum62 9 0.76 0.79
WP12 barc91 12 0.77 0.79
WP13 gwm304 11 0.74 0.77
WP14 gwm67 7 0.58 0.63
WP15 cfd29 15 0.83 0.84
WP16 gwm459 12 0.81 0.82
WP17 barc198 11 0.69 0.72
WP18 cfd76 13 0.79 0.81
WP19 cfa2028 8 0.51 0.56
WP20 gwm333 6 0.63 0.67
WP21 gwm437 16 0.63 0.65

Fig.2

Fingerprints of F-type wheat male sterile line"

Fig.3

UPGMA dendrogram of 442 wheat restorer lines Light color indicates cluster I, black color indicates cluster Ⅱ"

Fig.4

Genetic distance distribution between sterile line and restorer lines"

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