Crops ›› 2019, Vol. 35 ›› Issue (2): 78-83.doi: 10.16035/j.issn.1001-7283.2019.02.011

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Analysis of Genetic Diversity and Population Structure of Main Soybean Varieties in Heilongjiang Province

Chunyu Lin,Xiaoyu Liang,Huiyan Zhao,Yang Wang   

  1. Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, Heilongjiang, China
  • Received:2018-12-24 Revised:2019-02-11 Online:2019-04-15 Published:2019-04-12
  • Contact: Yang Wang

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Abstract:

In this study, 187 pairs of SSR markers were used to analyze the genetic diversity and population structure of 202 soybean cultivars planted in Heilongjiang Province in the past 25 years (1992-2017). A total of 808 polymorphic loci were produced from the genomic DNA of the varieties tested, and the average number of polymorphic loci was 4.42. Both satt703 and satt311 had the most polymorphic loci (10). Satt417 and satt575 had the most allelic variation frequency (99.5%). The genetic similarity coefficient of the varieties tested ranged from 0.283 to 0.930 with an average of 0.519. Some varieties bred by the same breeding unit had higher genetic similarity. 202 varieties were divided into three groups by population structure analysis, principal coordinate analysis (PCoA) and NJ clustering. The source of some of the materials in the group was not independent but interpenetrating.

Key words: Soybean, Heilongjiang, Genetic diversity, Population structure, SSR marker

Table 1

Breeding units and codes of test materials"

类型Type 育成单位Breeding unit 编号-材料Code-material
A

 东北农业大学大豆研究所

 1-东农24,2-东农36,3-东农38,4-东农42,5-东农43,6-东农47,7-东农48,8-东农49,9-东农50,10-东农51,11-东农52,12-东农53,13-东农54,14-东农55,15-东农56,16-东农57,17-东农5号,18-东农67
B 中国科学院东北地理与农业生态研究所 19-东升1,20-东升3号,21-东升9,22-东生7,23-东生8
C 黑龙江省农业科学院克山分院 24-丰收06,25-丰收11,26-丰收20,27-丰收24,28-丰收25
D








 黑龙江省农业科学院佳木斯分院








 29-合丰1,30-合丰14,31-合丰16,32-合丰23,33-合丰24,34-合丰25,35-合丰26,36-合丰28,37-合丰29,38-合丰32,39-合丰34,40-合丰36,41-合丰37,42-合丰38,43-合丰39,44-合丰41,45-合丰42,46-合丰43,47-合丰44,48-合丰46,49-合丰47,50-合丰48,51-合丰50,52-合丰51,53-合丰52,54-合丰53,55-合丰54,56-合丰55,57-合丰56,58-合农59,59-合农60,60-合农61,61-合农64,62合农65,63-合农66,64-合农68,65-合农75,66-合农76,67-合丰05,68-合丰06,69-合丰22,70-合丰30,71-合丰35,72-合丰40,73-合丰45,74-合丰57,75-合丰58,76-合丰60,77-合丰61,78-合丰62,79-合农62,80-合农63,81-合农67,82-合农69,83-合农70
E



 黑龙江省农业科学院黑河分院



 84-黑河35,85-金源55,86-黑河10,87-黑河11,88-黑河12,89-黑河14,90-黑河15,91-黑河16,92-黑河18,93-黑河19,94-黑河20,95-黑河21,96-黑河22,97-黑河23,98-黑河24,99-黑河25,100-黑河26,101-黑河33,102-黑河39,103-黑河43,104-黑河44,105-黑河46,106-黑河49,107-黑河50,108-黑河51,109-黑河52,110-黑河53,111-黑河56
F



 黑龙江省农业科学院大豆研究所



 112-黑农37,113-黑农40,114-黑农44,115-黑农48,116-黑农51,117-黑农52,118-黑农58,119-黑农60,120-黑农61,121-黑农62,122-黑农64,123-黑农65,124-黑农66,125-黑农68,126-黑农71,127-黑农83,128-龙豆1号,129-黑农69,130-黑农84,131-黑农85,132-哈05-5675,133-哈06-1939
G
 黑龙江省农业科学院绥化分院
 134-绥农22,135-绥农24,136-绥农26,137-绥农04,138-绥农25,139-绥农31,140-绥农35,141-绥农36,142-绥农38,143-绥农40,144-绥农41
H




 农垦地区育种单位




 145-北豆14,146-北豆5号,147-垦豆18,148-垦豆3,149-垦豆40,150-垦豆41,151-垦豆43,152-垦丰14,153-垦丰16,154-垦丰17,155-垦丰20,156-垦丰22,157-垦丰23,158-垦鉴27,159-垦鉴43,160-垦农23,161-垦农26,162-垦农28,163-北豆10,164-北豆19,165-北豆21,166-北豆22,167-北豆34,168-北豆41,169-北豆42,170-北豆47,171-北豆49,172-北丰11,173-北丰14,174-宝农1,175-垦豆25,176-垦豆38
I
 黑龙江省其他育种单位
 177-北疆2号,178-北疆九1,179-华疆2,180-华疆4,181-嫩奥1,182-嫩奥3,183-绥杨1号,184-北疆1,185-育丰115,186-育丰20,187-哈北46-1
J

 黑龙江省外育种单位

 188-登科1,189-登科8,190-登科5,191-赤豆1,192-赤豆3,193-俄豆-1,194-吉育95,195-长密30,196-长农18,197-长农24,198-长农26,199-长农27,200-长农28,201-长农29,202-长农31

Fig.1

LnP(D) and ΔK trend change based on K value"

Fig.2

Population structure of 202 soybean varieties"

Fig.3

Two-dimensional scatter plot of principal coordinate analysis of 202 soybean varieties based on SSR markers The material codes in the figure are the same as table 1, the same below"

Fig.4

Three-dimensional scatter plot of principal coordinate analysis of 202 soybean varieties based on SSR markers"

Fig.5

NJ clustering map of 202 soybean varieties"

[1] 夏正俊 . 大豆基因组解析与重要农艺性状基因克隆研究进展. 植物学报, 2017,52(2):148-158.
doi: 10.11983/CBB16087
[2] 栾晓燕, 杜维广, 满为群 , 等. 黑龙江省1986-2000年大豆育种研究成就与展望. 大豆科学, 2017(2):134-142.
doi: 10.3969/j.issn.1000-9841.2004.02.012
[3] 周静, 谷强平, 杜吉到 . 中国大豆进口依赖性及其对大豆进口安全的影响. 大豆科学, 2015,34(3):503-506,511.
doi: 10.11861/j.issn.1000-9841.2015.03.0503
[4] Liu Z X, Li J, Fan X H , et al. Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversity of soybean cultivars derived from Tokachi nagaha. The Crop Journal, 2017,5(4):326-336.
doi: 10.1016/j.cj.2016.11.001
[5] Jun T H, Michel A P, Mian M A . Development of soybean aphid genomic SSR markers using next generation sequencing. Genome, 2011,54(5):360-367.
doi: 10.1139/g11-002 pmid: 21529140
[6] 盖红梅, 李玉刚, 王瑞英 , 等. 鲁麦14对山东新选育小麦品种的遗传贡献. 作物学报, 2012,38(6):954-961.
doi: 10.3724/SP.J.1006.2012.00954
[7] 文自翔, 赵团结, 丁艳来 , 等. 中国栽培及野生大豆的遗传多样性、地理分化和演化关系研究. 科学通报, 2009,54(21):3301-3310.
[8] 秦君, 李英慧, 刘章雄 , 等. 黑龙江省大豆种质遗传结构及遗传多样性分析. 作物学报, 2009,35(2):228-238.
doi: 10.3724/SP.J.1006.2009.00228
[9] 马淑梅, 张宏纪, 孙岩 , 等. 俄罗斯远东及黑龙江省大豆种质资源遗传多样性和群体结构分析. 中国油料作物学报, 2017,39(1):23-29.
doi: 10.7505/j.issn.1007-9084.2017.01.004
[10] 何海燕, 沙伟, 张艳馥 . 黑龙江省大豆种质资源遗传多样性的ISSR分析. 大豆科学, 2011,30(1):37-40.
doi: 10.11861/j.issn.1000-9841.2011.01.0037
[11] 高运来, 姚丙晨, 刘春燕 , 等. 黑龙江省主栽大豆品种遗传多样性的SSR分析. 植物学报, 2009,44(5):556-561.
doi: 10.3969/j.issn.1674-3466.2009.05.005
[12] 陈斐 . 82份苜蓿种质资源的群体结构分析. 扬州:扬州大学, 2013.
[13] 王娟, 杜建材, 王照兰 , 等. 36份苜蓿种质材料遗传多样性的SSR分子标记. 中国草地学报, 2016,38(2):20-25,33.
doi: 10.16742/j.zgcdxb.2016-02-04
[14] 史晓红, 郭平仲 . 分子标记与小麦群体遗传结构分析. 首都师范大学学报(自然科学版), 2017(1):59-65.
doi: 10.3969/j.issn.1004-9398.2000.01.014
[15] 秦芳芳 . 大豆资源抗灰斑病1号生理小种优异等位变异的发掘. 哈尔滨:黑龙江大学, 2018.
[16] 陈庆山, 张忠臣, 刘春燕 , 等. 大豆主要农艺性状的QTL分析. 中国农业科学, 2017(1):41-47.
doi: 10.3969/j.issn.1004-8421.2017.01.023
[17] 时宽玉, 洪德林 . 6个水稻杂交组合与其亲本的SSR标记多态性及其应用. 南京农业大学学报, 2017(4):1-5.
doi: 10.3321/j.issn:1000-2030.2005.04.001
[18] 梁晓宇, 林春雨, 马淑梅 , 等. 水稻耐盐碱胁迫优异等位变异的发掘. 作物杂志, 2017(4):48-52.
[19] 金伟栋, 程保山, 洪德林 . 基于SSR标记的太湖流域粳稻地方品种遗传多样性研究. 中国农业科学, 2008,41(11):3822-3830.
doi: 10.3864/j.issn.0578-1752.2008.11.051
[20] 董永梅, 于航, 李有忠 , 等. 26份大豆品种(系)遗传多样性分析及指纹图谱构建. 分子植物育种, 2018,16(15):4964-4970.
[21] 何庆元, 向仕华, 杨华伟 , 等. 川渝地区地方和育成大豆品种SSR标记多样性分析. 中国油料作物学报, 2018,40(3):326-334.
doi: 10.7505/j.issn.1007-9084.2018.03.003
[22] 钟文娟, 袁灿, 周永航 , 等. 基于SSR标记的四川大豆与引进大豆资源遗传多样性和群体结构分析. 大豆科学, 2017,36(5):657-668.
doi: 10.11861/j.issn.1000-9841.2017.05.0657
[23] 陈维元, 吕德昌, 姜成喜 , 等. 绥农号大豆血缘关系分析. 黑龙江农业科学, 2017(4):9-12.
doi: 10.3969/j.issn.1002-2767.2004.04.003
[24] 胡喜平 . 合丰号大豆品种系谱分析. 大豆科学, 2017(2):131-137.
doi: 10.3969/j.issn.1000-9841.2002.02.012
[25] 盖钧镒, 汪越胜 . 中国大豆品种生态区域划分的研究. 中国农业科学, 2017(2):139-145.
doi: 10.3321/j.issn:0578-1752.2001.02.005
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