Crops ›› 2021, Vol. 37 ›› Issue (2): 173-177.doi: 10.16035/j.issn.1001-7283.2021.02.025

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Effects of Humic Acid on Ascorbate-Glutathione Cycle in the Leaves of Foxtail Millet Seedlings under Drought Stress

Shen Jie1(), Wang Yuguo2, Guo Pingyi2(), Yuan Xiangyang2   

  1. 1Faculty of Biology Science and Technology, Changzhi University, Changzhi 046011, Shanxi, China
    2College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
  • Received:2020-11-06 Revised:2021-02-22 Online:2021-04-15 Published:2021-04-16
  • Contact: Guo Pingyi E-mail:shijie216516@163.com;pyguo126@126.com

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Abstract:

To explore the regulation mechanism of humic acid on the antioxidant system of foxtail millet under drought stress, using Jingu 21 and Zhangza 10 as materials, the seed soaking method was used to study different concentrations of humic acid (0, 50, 100, 200, 300mg/L) on the ascorbate-glutathione (AsA-GSH) cycle of millet seedling leaves under drought stress. The results showed that 100 and 200mg/L humic acid treatment significantly increased the ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) activities of millet seedling leaves under drought stress, promoted the regeneration of AsA and GSH, increased the ratios of AsA/DHA and GSH/GSSH, and effectively alleviated the accumulation of reactive oxygen of millet seedlings. Therefore, humic acid could alleviate the oxidative damage of drought stress to the seedlings of foxtail millet by increasing the activity of metabolizing enzyme and the content of antioxidants in the AsA-GSH cycle, thus improved the drought resistance of foxtail millet.

Key words: Humic acid, Foxtail millet, Drought stress, AsA-GSH

Fig.1

Effects of HA on APX, GR, MDHAR and DHAR activities in foxtail millet seedling leaves Different lowercase letters indicate significant differences (P<0.05), the same below"

Table 1

Effects of HA on ascorbic acid contents in the seedlings of foxtail millet under drought stress nmol/μg FW"

品种Cultivar 处理Treatment 还原型抗坏血酸AsA 氧化型抗坏血酸DHA 总抗坏血酸Total AsA 还原型/氧化型抗坏血酸AsA/DHA
晋谷21号 CK 0.8961±0.0232d 1.7470±0.0179a 2.6431±0.0069d 0.5133±0.0183d
Jingu 21 T0 1.8989±0.0176c 1.3982±0.0404c 3.2971±0.0256c 1.3610±0.0508b
T1 1.9366±0.0192bc 1.5511±0.0308b 3.4877±0.0119b 1.2500±0.0371b
T2 1.9942±0.0296b 1.8407±0.0501a 3.8349±0.0205a 1.0858±0.0455c
T3 2.1807±0.0216a 1.2403±0.0403d 3.4210±0.0374b 1.7624±0.0683a
T4 1.9133±0.0317c 1.4234±0.0259c 3.3366±0.0235c 1.3457±0.0437b
张杂10号 CK 0.8615±0.0058d 1.3134±0.0326cd 2.1749±0.0324d 0.6568±0.0173f
Zhangza 10 T0 1.7480±0.0055c 1.3980±0.0257b 3.1460±0.0216c 1.2513±0.0259d
T1 1.7769±0.0097c 1.3259±0.0238bcd 3.1028±0.0141c 1.3412±0.0316c
T2 2.3249±0.0292a 1.3644±0.0201bc 3.6893±0.0107a 1.7053±0.0456a
T3 1.8383±0.0107b 1.7706±0.0136a 3.6089±0.0088b 1.0385±0.0131e
T4 1.8798±0.0087b 1.2697±0.0149d 3.1495±0.0076c 1.4810±0.0236b

Table 2

Effects of HA on glutathione contents in the seedlings of foxtail millet under drought stress nmol/mg FW"

品种Cultivar 处理Treatment 还原型谷胱甘肽GSH 氧化型谷胱甘肽GSSG 总谷胱甘肽Total GSH 还原型/氧化型谷胱甘肽GSH/GSSG
晋谷21号 CK 5.2814±0.0086c 0.6270±0.0060b 5.9084±0.0110c 8.4242±0.0802a
Jingu 21 T0 5.4825±0.0153b 0.7391±0.0074a 6.2216±0.0093b 7.4191±0.0928b
T1 5.4853±0.0088b 0.7444±0.0032a 6.2297±0.0101b 7.3693±0.0314b
T2 5.5566±0.0120a 0.7361±0.0075a 6.2927±0.0110a 7.5510±0.0863b
T3 5.5100±0.0087b 0.7266±0.0116a 6.2366±0.0058b 7.5872±0.1336b
T4 5.4762±0.0081b 0.7483±0.0187a 6.2245±0.0106b 7.3274±0.1930b
张杂10号 CK 5.2847±0.0118c 0.6028±0.0051c 5.8875±0.0079d 8.7686±0.0924a
Zhangza 10 T0 5.4956±0.0156b 0.7460±0.0156a 6.2416±0.0072c 7.3743±0.1735c
T1 5.5137±0.0075b 0.7356±0.0147a 6.2493±0.0099c 7.5017±0.1575c
T2 5.5683±0.0101a 0.7381±0.0023a 6.3064±0.0087a 7.5445±0.0343c
T3 5.5716±0.0081a 0.7053±0.0054b 6.2769±0.0102b 7.9010±0.0611b
T4 5.4970±0.0078b 0.7585±0.0040a 6.2554±0.0060bc 7.2480±0.0450c
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