Crops ›› 2021, Vol. 37 ›› Issue (4): 99-104.doi: 10.16035/j.issn.1001-7283.2021.04.015

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The Effects of NaCl Stress on Growth and Photosynthesis of Sugarbeet at Different Growth Stages

Wang Gang1,2(), Yu Lihua2, Zhao Huijie1,2, Liu Yu1,2, Geng Gui2()   

  1. 1College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2Crop Research Institute of Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2020-07-10 Revised:2020-09-09 Online:2021-08-15 Published:2021-08-13
  • Contact: Geng Gui E-mail:cqsz@outlook.com;genggui01@163.com

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Abstract:

In this study, sugarbeet seedlings were treated with 3 (CK), 140 (LS), and 280mmol/L Na+ (HS) during cotyledon stage, one pair of true leaves stage, and three pairs of true leaves stage to understand the difference of NaCl stress on sugarbeet seedlings at different stages. After NaCl treatment, growth indexes and photosynthetic physiological indexes of sugarbeet seedlings at different growth stages were measured. The results showed that the dry weight of sugarbeet seedlings under NaCl stress was significantly lower than that of CK and the range of change at cotyledon stage was more obvious than at the true leaf stages. The stomatal conductance (Gs), the transpiration rate (Tr), intercellular CO2 concentration (Ci), and net photosynthetic rate (Pn) of seedling leaves were significantly decreased by NaCl stress and the decrease at the cotyledon stage was significantly higher than that at true leaf stages. With the increase of NaCl concentration, the chlorophyll content also decreased significantly and decreased more at cotyledon stage than at the true leaf stages. In addition, NaCl stress resulted in decreases of Hill response and RuBPCase activity of sugarbeet seedlings. At the same time, it was found that PEPC activity increased significantly after LS stress with the maximum increase after three pairs of true leaves, but decreased to a certain extent after HS treatment. Therefore, with the increase of NaCl concentration, the growth and photosynthesis of sugarbeet seedlings were inhibited. With the growth of sugarbeet seedlings, the tolerance of the seedlings to NaCl stress was also increased.

Key words: Sugarbeet, NaCl stress, Photosynthesis

Fig.1

Changes of dry weight per plant of sugarbeet seedlings under different NaCl concentrations The datum are mean±SD, n=3; Different lowercase letters indicate significant difference at P < 0.05, the same below"

Table 1

Changes of RWC of sugarbeet seedling leaves under different NaCl concentrations"

处理Treatment 生长时期Growth stage 相对含水量RWC (%)
CK 子叶期 79.293±2.464c
一对真叶期 82.398±0.846a
三对真叶期 85.363±1.945b
LS 子叶期 67.168±6.798f
一对真叶期 68.640±0.836e
三对真叶期 69.589±0.402d
HS 子叶期 59.536±2.158h
一对真叶期 55.909±6.840i
三对真叶期 61.162±0.424g

Fig.2

Changes of chlorophyll content, Gs under different NaCl concentrations"

Fig.3

Changes of Tr and Ci under different NaCl concentrations"

Fig.4

Changes of Pn and Hill under different NaCl concentrations"

Table 2

Effects of different NaCl concentrations on the activities of RuBPCase and PEPC in sugarbeet leaves"

处理
Treatment		 生长时期
Growth stage		 RuBPCase
[μmolCO2/(min·g)FW]		 PEPC
[μmol/(mL·min)]
CK 子叶期 0.506±0.024d 0.134±0.008e
一对真叶期 0.554±0.025c 0.145±0.004d
三对真叶期 0.772±0.024a 0.127±0.008f
LS 子叶期 0.362±0.024g 0.153±0.004c
一对真叶期 0.410±0.025f 0.176±0.014a
三对真叶期 0.603±0.024b 0.184±0.002b
HS 子叶期 0.201±0.014i 0.092±0.008i
一对真叶期 0.248±0.014h 0.112±0.013h
三对真叶期 0.450±0.037e 0.114±0.006g
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