Crops ›› 2018, Vol. 34 ›› Issue (5): 85-90.doi: 10.16035/j.issn.1001-7283.2018.05.013

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QTL Mapping for Photosynthesis Related Traits in Upland Cotton

Tang Liyuan,Li Xinghe,Zhang Sujun,Wang Haitao,Liu Cunjing,Zhang Xiangyun,Zhang Jianhong   

  1. Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture/National Cotton Improvement Center Hebei Branch, P.R. China, Shijiazhuang 050051, Hebei, China
  • Received:2018-03-12 Revised:2018-05-07 Online:2018-10-15 Published:2018-10-12
  • Contact: Jianhong Zhang

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Abstract:

Photosynthesis provides a major material basis for the yield formation of cotton. A genetic linkage map was constructed for an F2 population of 196 cotton plants derived from a cross between two upland cotton parental lines of Jiyou 861 and Xinluzao 25 with SSR (Simple Sequence Repeat) markers. The map included 30 markers distributing in 4 linkage groups, full length 244.4cM. Based on the inclusive composite interval mapping method with QTL IciMapping 4.1, 10 QTLs (quantitative trait locus) were identified in the F2:3 population, including three QTLs for photosynthetic rates, two for stomatal conductance, one for intercellular CO2 concentration, one for transpiration rate and three for chlorophyll content, in which there were one photosynthetic rate QTL and one intercellular CO2 concentration QTL were located on chromosomes of D3 and D7, respectively. The research lays the foundation for fine mapping and cloning of QTL for photosynthesis related traits in cotton and provide a theoretical basis for combining molecular marker assisted breeding with high photosynthetic efficiency in cotton.

Key words: Cotton, Photosynthesis trait, Chlorophyll content, SSR marker, QTL mapping

Table 1

Photosynthesis related traits between parents and F2:3 populations of (Jiyou 861×Xinluzao 25)"

性状
Trait		 亲本Parent F2:3群体F2:3 population
冀优861
Jiyou 861		 新陆早25号
Xinluzao 25		 均值
Mean		 极小值
Minimum		 极大值
Maximum		 标准差
Standard deviation		 偏度
Skewness		 峰度
Kurtosis
Pn [μmol/(m2·s)] 24.71 13.52 14.74 8.32 25.28 3.24 0.47 0.22
Gs [μmol/(m2·s)] 0.24 0.20 0.18 0.08 0.32 0.04 0.34 0.17
Ci [μmol/mol] 206.29 246.36 246.48 171.53 324.32 25.69 0.10 0.28
Tr [μmol/(m2·s)] 4.95 3.33 3.43 1.66 5.26 0.88 0.05 -0.71
SPAD 53.05 43.50 49.53 39.98 58.52 2.75 -0.32 0.96

Fig.1

Normal distribution of photosynthesis related traits"

Table 2

Analysis of correlation among photosynthesis related traits in F2:3 population"

性状Trait Pn Gs Ci Tr
Gs 0.685**
Ci -0.582** 0.076
Tr 0.546** 0.814** -0.041
SPAD 0.127 0.004 -0.142* 0.000

Table 3

The analysis of QTL for photpsynthesis related traits in F2:3 population"

QTL 连锁群/染色体
Linkage group/Chromosome		 位置(cM)
Position		 左标记
Left marker		 右标记
Right marker		 LOD 加性效应
Additive effect		 显性效应
Dominant effect		 贡献率(%)
Contribution rate
qPn-1-1 LG1 44 STV031 SWU20241 2.06 2.74 1.50 8.17
qPn-1-2 LG1 98 SWU16417 BNL0830 2.12 2.81 -1.72 17.30
qPn-2-1 D3 22 SWU12703 SWU12406 3.20 -2.75 -0.09 22.87
qGs-1-1 LG1 45 STV031 SWU20241 2.23 0.04 0.00 20.55
qGs-1-2 LG1 140 SWU11499 SWU11689 2.06 -0.04 -0.04 17.11
qCi-4-1 D7 0 SWU10641 SWU10568 2.19 -15.39 -21.56 31.89
qTr-1-1 LG1 1 GL607 GL222 2.14 -0.11 1.30 3.31
qSPAD-1-1 LG1 1 GL607 GL222 3.16 0.73 -3.92 10.98
qSPAD-1-2 LG1 27 GL222 STV031 2.26 2.44 0.82 10.97
qSPAD-1-3 LG1 50 SWU20241 SWU11125 2.53 -0.38 2.98 5.90

Fig.2

The genetic linkage map and QTLs for photosynthesis related traits of (Jiyou 861×Xinluzao 25) "

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