Crops ›› 2021, Vol. 37 ›› Issue (2): 62-70.doi: 10.16035/j.issn.1001-7283.2021.02.009

;

Previous Articles     Next Articles

Study on High Temperature Stress Threshold of Maize Leaves

Zhang Xuepeng1(), Li Teng1, Wang Biao1, Liu Qing1, Liu Hanyu1, Tao Zhiqiang2(), Sui Peng1()   

  1. 1College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2020-11-11 Revised:2020-12-17 Online:2021-04-15 Published:2021-04-16
  • Contact: Tao Zhiqiang,Sui Peng E-mail:13001297977@163.com;taozhiqiang@caas.cn;suipeng@cau.edu.cn

RichHTML

25

PDF (PC)

1259

Abstract:

The present research aimed to find out the high temperature stress threshold of maize leaves. Using an artificial climate chamber with accurate temperature control, a pot experiment was designed. The daily maximum temperature of 32°C was used as the control, and three different daily maximum temperature treatments (34°C, 36°C, and 38°C) were set. The high temperature stress treatments were lasting ten days at the jointing stage. The response of photosynthetic photoreaction and dark reaction phases of leaves at different temperatures, and the ultrastructure of stomata and chloroplasts of leaves were analyzed. At the photoreaction phase, the maximum light efficiency of photosystem Ⅱ and the actual photochemical efficiency treatment were significantly reduced under the treatment of 38°C, and both indicators returned to normal levels after releasing of the high temperature stress. But the other three treatments showed no significant difference. At the dark reaction phase, the net photosynthetic rates at 36°C and 38°C were significantly lower than that of the control, and the intercellular CO2 concentration increased significantly, and the net photosynthetic rate at 38°C could not return to normal level after releasing of high temperature stress. The results of the transmission electron microscope showed that under the treatment of 36°C and 38°C, the chloroplast structure gradually disordered and degraded, the content of the lipid sphere increased, and the synthesis of starch decreased. Therefore, for the leaf of maize, the threshold of high daily maximum temperature stress was 36°C. In addition, high temperature stress near the threshold mainly restricts the dark reaction stage of photosynthesis.

Key words: Maize, High temperature stress, Threshold, Leaf, Photosynthesis, Chloroplast

Table 1

Temperature of climate room from 8∶00-18∶00 in experiment conducted ℃"

日最高温Daily maximum temperature 8∶00 9∶00 10∶00 11∶00 12∶00 13∶00 14∶00 15∶00 16∶00 17∶00 18∶00
32 27 28 29 30 31 32 32 32 31 30 28
34 29 30 31 32 33 34 34 34 33 32 30
36 31 32 33 34 35 36 36 36 35 34 32
38 33 34 35 36 37 38 38 38 37 36 34

Fig.1

Changes of temperature in climate room under different treatments"

Fig.2

Changes of chlorophyll fluorescence parameters of maize leaf under different temperature treatments P: pre-treatment; 1h: the maximum temperature lasted for 1h on day 1; 1d, 2d, 3d, 6d ,10d: the maximum temperature lasted for 2h on day 1, 3, 6, and 10d, respectively; 17d: 7d after treatment; the significance level of difference is P < 0.05. The same below"

Fig.3

Changes of photosynthetic parameters of maize leaves under different temperature treatments"

Fig.4

Effects of different temperatures on lower epidermal structure and stomata status of maize leaves"

Fig.5

Effects of different temperatures on chloroplast status in mesophyll cell of maize leaves Ch: chloroplast; M: mitochondria; Cn: cell nucleus; Sg: starch grains; P: plastoglobuli; L: lysosome; St: sponge tissue; Pt: palisade tissue; Bs: bundle sheath"

Fig.6

Effects of different temperatures on chloroplast status in mesophyll cell of maize leaves Cw: cell wall; Ch: chloroplast; M: mitochondria; Th: thylakoid lamella; Sg: starch grains; P: plastoglobuli"

[1] Nations FAO. FAO Statistical Pocketbook 2015:World Food and Agriculture:Rome, 2015.
[2] IPCC. Climate Change 2014. Cambridge and New York: Cambridge University Press, 2014.
[3] Schlenker W, Roberts M J. Nonlinear temperature effects indicate severe damages to US crop yields under climate change. Proceedings of the National Academy of Sciences of the United States of America, 2009,106(37):15594-15598.
[4] Lobell D B, Baenziger M, Magorokosho C, et al. Nonlinear heat effects on African maize as evidenced by historical yield trials. Nature Climate Change, 2011,1(1):42-45.
[5] Hawkins E, Fricker T E, Challinor A J, et al. Increasing influence of heat stress on French maize yields from the 1960s to the 2030s. Global Change Biology, 2013,19(3):937-947.
[6] 赵鸿, 王润元, 尚艳, 等. 粮食作物对高温干旱胁迫的响应及其阈值研究进展与展望. 干旱气象, 2016,34(1):1-12.
[7] 于振文. 作物栽培学各论. 中国农业出版社, 2003.
[8] Zhang J H, Huang W D, Liu Y P, et al. Effects of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L. cv. Jingxiu) under cross-temperature stresses. Journal of Integrative Plant Biology, 2005,47(8):959-970.
[9] Camejo D, Rodriguez P, Morales A, et al. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility. Journal of Plant Physiology, 2005,162(3):281-289.
[10] Faria T, GarciaPlazaola J I, Abadía A, et al. Diurnal changes in photoprotective mechanisms in leaves of cork oak (Quercus suber) during summer. Tree Physiology, 1996,16(1/2):115-123.
[11] Stroch M, Vrabl D, Podolinska J, et al. Acclimation of Norway spruce photosynthetic apparatus to the combined effect of high irradiance and temperature. Journal of Plant Physiology, 2010,167(8):597-605.
[12] Li M F, Ji L S, Yang X H, et al. The protective mechanisms of CaHSP26 in transgenic tobacco to alleviate photoinhibition of PSⅡ during chilling stress. Plant Cell Reports, 2012,31(11):1969-1979.
[13] Peng H T, Li Y X, Zhang C W, et al. Chloroplast elongation factor BcEF-Tu responds to turnip mosaic virus infection and heat stress in non-heading Chinese cabbage. Biologia Plantarum, 2014,58(3):561-566.
[14] Way D A, Sage R F. Thermal acclimation of photosynthesis in black spruce [Picea mariana (Mill.) BSP]. Plant Cell and Environment, 2008,31(9):1250-1262.
[15] 郝召君. 芍药对高温胁迫的响应及其化学调控机制研究. 扬州:扬州大学, 2017.
[16] Sharkey T D, Zhang R. High temperature effects on electron and proton circuits of photosynthesis. Journal of Integrative Plant Biology, 2010,52(8):712-722.
[17] 赵薇, 李贞, 王谦, 等. 冬小麦灌浆期旗叶光曲线及叶绿素荧光参数研究. 气象与环境科学, 2017,40(1):64-72.
[18] 董轲. 低温光胁迫对植物光系统的伤害及缓解机理研究. 济南:山东师范大学, 2017.
[19] 李新国. 低温弱光条件下喜温植物PSI和PSII的光抑制及其相互关系. 泰安:山东农业大学, 2003.
[20] 赵龙飞, 李潮海, 刘天学, 等. 花期前后高温对不同基因型玉米光合特性及产量和品质的影响. 中国农业科学, 2012,45(23):4947-4958.
[21] 曲明南. CO2升高和短期高温胁迫对玉米幼苗生理生化指标的影响. 沈阳:沈阳农业大学, 2013.
[22] 路涛. 亚高温强光胁迫下番茄幼苗光抑制及光保护机制研究. 沈阳:沈阳农业大学, 2016.
[23] 黄纯倩, 朱晓义, 张亮, 等. 干旱和高温对油菜叶片光合作用和叶绿素荧光特性的影响. 中国油料作物学报, 2017,39(3):342-350.
[24] 季浩. 不同生育期及高温对玉米和棉花叶片叶绿素荧光参数的影响研究. 南京:南京农业大学, 2017.
[25] 孙胜楠, 王强, 孙晨晨, 等. 黄瓜幼苗光合作用对高温胁迫的响应与适应. 应用生态学报, 2017,28(5):1603-1610.
[26] 高冠龙, 冯起, 张小由, 等. 植物叶片光合作用的气孔与非气孔限制研究综述. 干旱区研究, 2018,35(4):929-937.
[27] Kanechi M, Uchida N, Yasuda T, et al. Non-stomatal inhibition associated with inactivation of Rubisco in dehydrated coffee leaves under unshaded and shaded conditions. Plant and Cell Physiology, 1996,37(4):455-460.
[28] Wise R R, Olson A J, Schrader S M, et al. Electron transport is the functional limitation of photosynthesis in field-grown Pima cotton plants at high temperature. Plant Cell and Environment, 2004,27(6):717-724.
[29] 王德权. 持绿型高粱、玉米对干旱胁迫响应的生理机制比较研究. 沈阳:沈阳农业大学, 2012.
[30] 张华, 翁梦苓, 梁志敏, 等. 烟草腺毛发育过程中叶绿体形态学研究. 西北植物学报, 2008(8):1592-1595.
[31] 时向东, 方圆, 杨双剑, 等. 烤烟叶片发育过程中的组织学和细胞学研究. 烟草科技, 2009(7):48-52.
[32] 时向东, 焦枫, 范豪杰, 等. 烤烟叶片发育过程中栅栏细胞超微结构的变化. 烟草科技, 2010(5):46-47.
[33] 张彩霞. 高温影响水稻韧皮部同化物转运及代谢的作用机制及调控. 北京:中国农业科学院, 2018.
[34] Yamori W, Hikosaka K, Way D A. Temperature response of photosynthesis in C3,C4,and CAM plants:temperature acclimation and temperature adaptation. Photosynthesis Research, 2014,119(1/2):101-117.
[1] Wang Xiaolei, Zhang Yunhe, Mu Jinmeng, Gao Dapeng, Geng Yanqiu, Cao Yiwen, Lu Fen, Guan Zhengwen, Shao Xiwen, Guo Liying. Effects of Soda and Saline-Alkali Stress on Photosynthetic Characteristics and Yield of Rice [J]. Crops, 2024, 40(1): 193-203.
[2] Feng Yong, Hou Xuguang, Xue Chunlei, Zhang Laihou, Song Guodong, Su Minli, Fu Xiaohua, Sun Yuyan. Division of Suitable Ecological Regions of Maize Varieties in Inner Mongolia [J]. Crops, 2024, 40(1): 23-30.
[3] Wang Haitao, Ren Chunmei, Dong Yan, Li Shuo, Cheng Zhaobang, Ji Yinghua. Molecular Detection and Identification of Maize Yellow Mosaic Virus on Sorghum in Huai’an, Jiangsu [J]. Crops, 2024, 40(1): 233-238.
[4] Ma Juan, Huang Lu, Yu Ting, Guo Guojun, Zhu Weihong, Liu Jingbao. Multi-Locus Genome-Wide Association Study and Genomic Prediction for General Combining Ability of Maize Ear Diameter [J]. Crops, 2024, 40(1): 31-39.
[5] Xie Keran, Gao Ti, Cui Kehui. Research Progress of Potassium Fertilizer Controlling Rice Yield under High Temperature [J]. Crops, 2024, 40(1): 8-15.
[6] Wu Ying, Hu Die, Li Ting, Duan Qianyuan, Wei Ningning, Zhang Xinghua, Xu Shutu, Xue Jiquan. Analysis of WRKY Transcription Factor IIc Subfamily in Maize and Its Expression Profile under Drought [J]. Crops, 2024, 40(1): 80-89.
[7] Jin Yu, Guo Xinyu, Zhang Ying, Li Dazhuang, Wang Jinglu. Stomatal Phenotypic Identification and Research Progress in Maize Leaves [J]. Crops, 2023, 39(6): 1-10.
[8] Wu Qi, Ming Bo, Gao Shang, Yang Hongye, Zhang Chuan, Chu Zhendong, Li Shaokun. Research on the Construction Strategy of Maize Grain Dehydration Model in Cold Northeast China [J]. Crops, 2023, 39(6): 108-113.
[9] Liang Zhongyu, Xue Jun, Zhang Guoqiang, Ming Bo, Shen Dongping, Fang Liang, Zhou Linli, Zhang Yuqin, Yang Hengshan, Wang Keru, Li Shaokun. Effects of Phosphorus Application Rate on Lodging Resistance of Maize under Integrated Water and Fertilizer [J]. Crops, 2023, 39(6): 190-194.
[10] Liu Hui, Long Xueyi, Jiao Yan, Wang Lihong. Effects of Combined Application of Biochar and Phosphate Fertilizer on Rice Growth and Yield [J]. Crops, 2023, 39(5): 238-248.
[11] Cao Qingjun, Li Gang, Yang Hao, Lou Yuyong, Yang Fentuan, Kong Fanli, Li Xinbei, Zhao Xinkai, Jiang Xiaoli. The Effects of Different Tillage Practices on Seedbed Quality and Its Relationships with Seedling Population Construction and Grain Yield of Spring Maize [J]. Crops, 2023, 39(5): 249-254.
[12] Yu Le, Li Lin, Huang Hongjuan, Huang Zhaofeng, Zhu Wenda, Wei Shouhui. Weed Species Composition and Community Characterization in Maize Fields in Hubei Province [J]. Crops, 2023, 39(5): 272-279.
[13] Yang Zongying, Xiao Gui, Zhang Hongwei. Whole-Genome Predictive Analysis of Fresh Weight per Plant Using the Maize F1 Population [J]. Crops, 2023, 39(5): 43-48.
[14] Qu Haitao, Li Zhongnan, Wang Yueren, Ma Yiwen, Xiang Yang, Wu Shenghui, Tan Zhuo, Wang Chun, Wei Qiang, Luo Yao, Li Guangfa. Study on Genetic and Breeding Effects of 100-Grain Weight in Maize [J]. Crops, 2023, 39(5): 66-70.
[15] Yang Mi, Wang Meijuan, Xu Haitao. Study on the Dynamic Development Difference of Husk of Maize Inbred Lines in Different Ecological Regions [J]. Crops, 2023, 39(5): 81-90.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!